Τόμος 5 (1991) – Τεύχος 1 – Άρθρο 2 – Επιθεώρηση Κλινικής Φαρμακολογίας και Φαρμακοκινητικής-Διεθνής Έκδοση – Volume 5 (1991) – Issue 1 – Article 2 – Epitheorese Klinikes Farmakologias και Farmakokinetikes-International Edition

Laboratory for Enzyme Research, Institute of Biology, National Center for Scientific Research “Demokritos”, Athens 15310, Greece

Accepted for publication: January 15, 1991

pharmakonpress[at]pharmakonpress[.]gr

2.       Ham, R.G.: Survival and growth requirements of non-transformed cells. In: Tissue Growth Factors (R. Baserga, ed.), p. 13, Springer-Verlag, Berlin, 1981

3.       Balk, S.D.: Calcium as regulator of the proliferation of normal but not of transformed, chicken fibroblasts in plasma-containing medium. Proc. Natl. Acad. Sci. USA 68: 271 (1971)

4.       Balk, S.D., Whittfield, J.F., Youdale, T., Braun, A.C.: Role of calcium, serum, plasma and folic acid in the control of proliferation of normal and Rous sarcoma virus infected chicken fibroblasts. Proc. Natl. Acad. Sci. USA. 70: 675 (1973)

5.       Ross, R„ Glomset, J., Kariya, B., Harker, L: A platelet-dependent serum factor that stimulates the proliferation of arterial smooth muscle cells in vitro. Proc. Natl. Acad Sci. USA. 71: 1207 (1974)

6.       Kohler, N., Lipton, A.: Platelets as a source of fibroblast growth-promoting activity. Exp. Cell Res. 87: 297 (1974)

7.       Scher, C.D., Stathakos, D., Antoniades, H.N.: Dissociation of cell division stimulating capacity for Balb/c 3T3 from insulin-like activity in human serum. Nature 247: 279 (1974)

8.       Antoniades, H.N., Stathakos, D., Scher, C.D.: Isolation of a cationic polypeptide from human serum that stimulates proliferation of 3T3 cells. Proc. Natl. Acad. Sci. USA 72: 2635 (1975)

9.       Antoniades, H.N., Scher, C.D.: Radioimmunoassay of a human serum growth factor for Balb/c 3T3 cells: derivation from platelets. Proc. Natl. Acad. Sci. USA 74: 1973 (1977)

10.     Waterfield, M.D., Scrace, G.T., Whittle, N., Stroobant, P., Johnsson, A., Wasteson, A., Westermark, B., Heldin, C.-H., Huang, J.S., Deuel, T.F.: Platelet-derived growth factor is structurally related to the putative transforming protein p28 of simian sarcoma virus. Nature 304: 35 (1983)

11.     Doolittle, R.F., Hunkapiller, M.W., Hood, LE., Devare, S.G., Robbins, K.C., Aaronson, S.A., Antoniades, H.N.: Simian sarcoma virus one gene, v-sis, is derived from the gene (or genes) encoding a platelet-derived growth factor. Science 221: 275 (1983)

12.     Heldin, C.-H., Westermark, B.: Growth factors as trans-forming proteins. Eur. J. Biochem. 184: 487 (1989)

13.     Sorrentino, V.: Growth factors, growth inhibitors and cell cycle control. Anticancer Res. 9: 1925 (1989)

14.     Sporn, M.B., Roberts, A.B.: Peptide growth factors are multifunctional. Nature 332: 217 (1988)

14a. Sporn, M.B., Roberts, A.B.: The transforming growth factor-betas: Past, present and future. Ann. N.Y. Acad. Sci. 593: 1 (1990)

15.     Ross, R.: The platelet-derived growth factor. In: Tissue Growth Factors (R. Baserga, ed.), p. 133, Springer-Verlag, Berlin, 1981

16.     Hawiger, J.: Platelet secretory pathways: An overview Meth. Enzymol. 7 69: 191 (1989)

17.     Sixma, J.J., Slot, J.-W., Genze, H.J.: Immunocytochemical localization of platelet granule proteins. Meth. Enzymol. 169: 301 (1989)

18.     Van Dam-Mieras, M.C.E., Muller, A.D.: Blood coagulation as a part of the haemostatic system, in: Blood Coagulation (Zwall R.F.A., Hemker, H.C., eds), p. 1, Elsevier Science Publishers B.V., 1986

19.     Zucker, M.B.: Platelet aggregation measured by the photometric method. Meth. Enzymol. 169: 117 (1989)

20.     Mustard, J.F., Kinlough-Rathbone, R.L, Packham, M.A.: Isolation of human platelets from plasma by centrifugation and washing. Meth. Enzymol. 169: 3 (1989)

21.     Ross, R., Nist, C., Kariya, B., Rivest, M., Raines, E., Callis, J.: Physiological quiescence in plasma-derived serum: Influence of platelet-derived growth factor on cell growth in culture. J. Cell Physiol. 97: 497 (1978)

22.     Holt, J.C., Harris, M.E., Holt, A.M., Lange, E., Henschen, A., Niewiarowski, S.: Characterization of human platelet basic protein, a precursor form of low-affinity platelet factor 4 and p-thromboglobulin. Biochemistry 25: 1988 (1986)

23.     Ross, R., Raines, E.W., Bowen-Pope, D.F.: The biology of platelet-derived growth factor. Cell 46: 155 (1986)

24.     Ross, R.: Peptide regulatory factors: PDGF. Lancet 1179 (1989)

25.     Westermark, B.: The molecular and cellular biology of platelet-derived growth factor. Acta Endocrinoiogica 123: 131 (1990)

26.     Antoniades, H.N., Scher, C.D., Stiles, C.D.: Purification of human platelet-derived growth factor. Proc. Natl. Acad. Sci. USA. 76: 1809 (1979)

27.     Raines, E.W., Ross, R.: Platelet-derived growth factor: I. High yield purification and evidence for multiple forms. J. Biol. Chem. 257: 5154 (1982)

28.     Heldin, C.-H., Westermark, B., Wasteson, A.: Platelet- derived growth factor isolation by a large scale procedure and analysis of subunit composition. Biochem. J. 193: 907 (1981)

29.     Antoniades, H.N., Pantazis, P.: Platelet-derived growth factor: Purification and characterization. Meth. Enzymol 169: 210 (1989)

30.     Heldin, C.-H., Johnsson, A., Ek, B., Wennergren, S., Rönnstrand, L., Hammacher, A., Faulders, B., Wasteson, A., Westermark, B.: Purification of human pla- telet-derived growth factor. Meth. Enzymoi 147: 3 (1987)

31.     Heldin, C.-H., Westermark, B., Wasteson, A.: Demon-stration of an antibody against platelet-derived growth factor. Exp. Cell Res. 136: 255 (1981)

32.     Di Corleto, P.E., Bowen Pope, D.F.: Cultured endothelial cells produce a platelet-derived growth factor-like protein. Proc. Natl. Acad. Sci. USA 80: 1919 (1983)

33.     Seifert, R.A., Schwartz, S.M., Bowen Pope, D.F.: Developmentally regulated production of platelet-derived growth factor-like molecules. Nature 311: 669 (1984)

34.     Shimokado, K., Raines, E.W., Madtes, D.K., Barett, T.B., Benditt, E.P., Ross, R.: A significant part of macrophage-derived growth factor consists of at least two forms of PDGF. Cell 43: 277 (1985)

35.     Goustin, A.S., Betscholz, C., Pfeifer-Ohlsson, S., Pers- son, H., Rydnert, J., Bywater, M., Holmgren, G., Heldin, C.-H., Westermark, B., Ohlsson, R.: Coexpression of the sis and myc protooncogenes in human placenta suggest autocrine control of trophoblast growth. Cell 41: 301 (1985)

36.     Paulsson, Y„ Hammacher, A., Heidin, C.-H., Westermark, B.: Possible positive autocrine feedback in the prereplicative phase of human fibroblasts. Nature 328: 715 (1987)

37.     Pantazis, P., Sariban, E., Kufe, D., Antoniades, H.N.: Induction of c-sis gene expression and synthesis of platelet-derived growth factor in human myeloid leukemia cells during monocytic differentiation. Proc. Natl. Acad. Sci USA 83: 6455 (1986)

38.     Pantazis, P., Pelicci, P.G., Dalla-Favera, R., Antoniades, H.N.: Synthesis and secretion of proteins resembling platelet-derived growth factor by human glioblastoma and fibrosarcoma cells in culture. Proc. Natl. Acad. Sci USA 82: 2404 (1985)

39.     Westermark, B., Johnsson, A., Paulsson, Y., Betsholtz, C., Heldin, C.-H., Herlyn, M., Rodeck, Κ., Koprowski, H.: Human melanoma cell lines of primary and metastatic origin express the genes encoding the chains of platelet-derived growth factor and produce a PDGF-like growth factor. Proc. Natl. Acad. Sci USA 83: 7197 (1986)

40.     Betsholtz, C„ Heldin, C.-H., Nister, M., Ek B., Waste- son, A., Westermark, B.: Synthesis of a PDGF-like growth factor in human glioma and sarcoma cells suggests the expression of the cellular homologue to the transforming protein of simian sarcoma virus. Biochem. Blophys. Res. Commun. 117: 176 (1983)

41.     Raines, E.W., Ross, R.: Purification of human PDGF. Meth. Enzymol. 109: 749 (1985)

42.     Stenman, G., Rorsman, F., Betsholtz, C.: Sublocalization of the human PDGF A-chain gene to chromosome-2 band q 11.23 by in situ hybridization. Exp. Cell Res. 178: 180 (1988)

43.     Johnsson, A., Heldin, C.-H., Westermark, B., Wasteson, A.: Platelet-derived growth factor: Identification of constituent polypeptide chains. Biochem. Biophys. Res. Commun. 104: 66 (1982)

44.     Johnsson, A., Heldin, C.-H., Wasteson, A., Westermark, B.,  Deuel, T.F., Huang, J.S., Seeburg, P.H., Gray, A., Ullrich, A., Scrace, G., Stroobant, P., Waterfield, M.D.: The c-sis gene encodes a precursor of the B-chain of platelet derived growth factor. EMBO J. 3: 921 (1984)

45.     Heldin, C.-H., Johnsson, A., Wennergren, S., Wernstedt, C., Betsholtz, C., Westermark, B.: A human osteosarcoma cell line secretes a growth factor related to a homodimer of PDGF-A-chains. Nature 319: 511 (1986)

46.     Hammacher, A., Nister, M., Westermark, B., Heldin, C.-H.: A human glioma cell line secretes three structurally and functionally different dimeric forms of platelet-derived growth factor. Eur. J. Biochem. 176: 179 (1988)

47.     Poggi, A., Rucinski, B., James, P., Holt, J.C., Niewtarowski, S.: Partial purification and characterization of porcine platelet-derived growth factor. Exp. Cell Res. 150: 436 (1984)

48.     Hammacher, A., Heilman, Κ., Johnsson, A. Ostman, A., Gunnarson, Κ., Westermark, B., Wasteson, A., Heldin, C.-H.: A major part of platelet-derived growth factor purified from human platelets is a heterodimer of one A and one B chain. J. Biol. Chem. 263: 16493 (1988)

49.     Hart, C.E., Bailey, M., Curtis, P.A., Osborn, S., Raines, E., Ross, R., Forstrom, J.W.: Purification of PDGF-AB and PDGF-BB from human platelet extract and identification of all three PDGF dimers in human platelets. Biochemistry 29: 166 (1990)

50.     Claesson-Welsh, L, Eriksson, A., Moren, A., Severins- son, L., Ek, B., Ostman, A., Betsholtz, C., Heldin, C.-H.: c-DNA cloning and expression of a human platelet- derived growth factor receptor specific for B-chain-containing PDGF molecules. Mol. Cell Biol. 8: 3476 (1988)

51.     Ostman, A., Rail, L., Hammacher, A., Wormstead, M.A., Coit, O., Valenzuela, P., Betsholtz, C., Westermark, B., Heldin, C.-H.: Synthesis and assembly of a functionally active recombinant platelet-derived growth factor heterodimer. J. Biol. Chem. 263: 16202 (1988)

52.     Thyberg, J., Ostman, A., Bäckström, Westermark, B., Heidin, C.-H.: Localization of platelet-derived growth factor (PDGF) in CHO cells transfected with PDGF A- or B-chain DNA: retention of PDGF-BB in the endoplasmic reticulum and Golgi complex. J. Cell Sci. 97: 219 (1990)

53.     Bowen-Pope, D.F., Malpass, T.W., Foster, D.M., Ross, R.: Platelet-derived growth factor in vivo: Levels, activity and rate of clearance. Blood 64: 458 (1984)

54.     Raines, E.W., Ross, R.: Identification and assay of platelet-derived growth factor-binding proteins. Meth. Enzymol. 147: 48 (1987)

55.     Sottrup-Jensen, L.: α-macroglobulins: structure, shape and mechanisms of proteinase complex formation. J. Biol. Chem. 264: 11539 (1989)

56.     Barret, A.J., Starkey, P.M.: The interaction of a2 macroglobulin with proteases: Characteristics and specificity of the reaction and a hypothesis concerning its molecular mechanism. Biochem. J. 133: 709 (1973)

57.     Huang, J.S., Haung, S.S., Deuel, T.F.: Human PDGF Radioimmunoassay and discovery of a specific plasma-binding protein. J. Cell Biol. 97: 383 (1983)

58.     Raines, E.W., Bowen-Pope, D.F., Ross, R.: Plasma binding proteins for platelet-derived growth factor that inhibits its binding to cell-surface receptors. Proc. Natl. Acad. Sci. USA 81: 3424 (1984)

59.     Huang, J.S., Huang, S.S., Deuel, T.F.: Specific covalent binding of platelet-derived growth factor to human plasma a2 macroglobulin. Proc. Natl. Acad. Sci. USA 81: 342 (1984)

60.     Bonner, J.C., Badgett, A., Osornio-Vargas, A.R., Hoff-man, M., Brody, A.R.: PDGF-stimulated fibroblast proliferation is enhanced synergistically by receptor-recognized a2 macroglobulin. J. Cell. Physiol. 145: 1 (1990)

61.     Dennis, P.A., Saksela, O., Harpel, P., Rifkin, D.B.: a2 macroglobulin is a binding protein for basic-fibroblast growth factor. J. Biol. Chem. 264: 7210 (1989)

62.     Roune, H., Anundi, H., Rask, L., Peterson, P.A.: Nerve growth factor binds to serum a2-macroglobulm. Biochem. Biophys. Res. Commun. 87: 330 (1976)

63.     0 ‘Connor-McCourt, M.D., Wakefield, L.M.: Latent transforming growth factor-ß in serum. A specific complex with a2-macroglobulin. J. Biol. Chem. 262: 14090 (1987)

64.     Hart, C.E., Forstrom, J.W., Kelly, J.D., Seifert, R.A., Smith, R.A., Ross, R., Murray, M.J., Bowen-Pope, D.F.: Two classes of PDGF receptor recognize different isoforms of PDGF. Science 240: 1529 (1988)

65.     Heldin, C.-H., Bäckström, G., Ostman, A., Hammacher, A., Rönnstrand, L, Rubin, Κ., Nister, M., Wester mark, B.: Binding of different dimeric forms cPDGF to human fibroblasts: evidence for two separate receptor types. EMBO J. 7: 1387 (1988)

66.     Westermark, B., Claesson-Welsh, L., Heldin, C.-H.: Structural and functional aspects of platelet-derived growth factor and its receptors, in Proto-oncogenes in cell development. In: Ciba Foundation Symposium 150, (Bock G., March, J., eds), p. 6, Wiley, New York, 1990

67.     Heldin, C.-H., Ernlund, A., Rorsman, C., Rônnstrand, L.: Dimerization of B-type platelet-derived growth factor receptors occurs after ligand binding and is closely associated with receptor kinase phosphorylation. J. Biol. Chem. 89: 8905 (1989)

68.     Bishayee, J., Majumbar, S., Khine, J., Das, M.: Ligand induced dimerization of the platelet-derived growth factor receptor Monomer-dimer interconversion occurs independent of receptor phosphorylation. J. Biol. Chem. 264: 11699 (1989)

69.     Seifert. R.A., Hart, G.E., Phillips, P.E., Forstrom, J.W., Ross, R., Murray, M.J., Bowen-Pope, D.F.: Two different subunits associate to create isoform specific platelet- derived growth factor receptors. J. Biol. Chem. 264: 8771 (1989)

70.     Mellstrôm, Κ., Heldin, C.-H., Westermark, B.: Induction of circular membrane ruffling on human fibroblasts by platelet-derived growth factor. Exp. Cell Res. 177: 347 (1988)

71.     Heldin, C.-H., Westermark, B.: Signal transduction by the receptors for platelet-derived growth factor. J. Cell Sci. 96: 193 (1990)

72.     Cooper, J.A., Bowen-Pope, D.F., Raines, E., Ross, R„ Hunter, T.: Similar effects of platelet-derived growth factor and epidermal growth factor on the phosphorylation of tyrosine in cellular proteins. Cell 31: 263 (1982)

73.     Rozengurt, E.: Early signals in the mitogenic response. Science 234: 161 (1986)

74.     Berridge, M., Heslop, J., Irvine, R.F., Brown, K.D.: Inositol triphosphate formation and calcium mobilization in Swiss 3T3 cells in response to platelet-derived growth factor. Biochem. J. 222: 195 (1984)

75.     Habenicht, A.J.R., Glomset, J.A., King, W.C., Nist, C., Mitchell, C.D., Ross, R.: Early changes in phosphatidylinositol and arachidonic acid metabolism in quiescent Swiss 3T3 cells stimulated to divide by platelet- derived growth factor. J. Biol. Chem. 256: 12229 (1981)

76.     Habenicht, A.J.R., Goerig, M., Grulich, J., Rothe, D., Gronwald, R., Loth, U., Schettler, G., Kommerell, B., Ross, R.: Human platelet-derived growth factor stimu-lates prostaglandin synthesis by activation and by rapid de novo synthesis of cyclooxygenase. J. Clin. Invest. 75: 1381 (1985)

77.     Nishizuka, Y.: The role of protein kinase C in cell surface signal transduction and tumour promotion. Nature 308:693 (1984)

78.     Berridge, M.J.: Inositol triphosphate and diacylglycerol: two interacting second messengers. Ann. Rev. Biochem. 56: 159 (1987)

79.     Armelin, H.A., Armelin, M.C.S., Kelly, K., Stewart, T., Leder, P., Cochran, B.H., Stiles, C.D.: Functional role for c-myc in mitogenic response to platelet-derived growth factor. Nature 310: 655 (1984)

80.     Siegfried, Z., Ziff, E.B.: Transcription activation by serum, PDGF and TPA through the c-fos DSE: Cell type specific requirements for induction. Oncogene 4: 3 (1989)

81.     Greenberg, M.E., Ziff, E.B.: Stimulation of 3T3 cells induces transcription of the c-fos proto-oncogene. Nature 311: 433 (1984)

82.     Seppä, H., Grottendorst, G., Schiffmann, E., Martin, G.R.: Platelet-derived growth factor is chemotactic for fibroblasts. J. Cell Biol. 92: 584 (1982)

83.     Grottendorst, G.R., Seppä, H.E.J., Kleinman, H.K., Martin, G.R.: Attachment of smooth muscle cells to collagen and their migration toward platelet-derived growth factor. Proc. Natl. Acad. Sci. USA 78: 3669 (1981)

84.     Deuel, T.F., Senior, R.M., Huang, J.S., Griffin, G.L.: Chemotaxis of monocytes and neutrophils to platelet-derived growth factor. J. Clin. Invest. 69: 1046 (1982)

85.     Tzeng, D.Y., Deuel, T.F., Huang, J.S., Baechner, R.L.: Platelet-derived growth factor promotes human peripheral monocyte activation. Blood 66: 179 (1985)

86.     Graves, D.I., Grottendorst, G.R., Antoniades, H.N., Schwartz, C.J., Valente, A.J.: Platelet-derived growth factor is not chemotactic for human peripheral blood monocytes. Exp. Cell Res. 180: 497 (1989)

87.     Clowes, A.W., Schwartz, S.M.: Significance of quiescent smooth muscle migration in the injured rat carotid artery. Circ. Res. 56: 139 (1985)

8B. Ross, R.: The pathogenesis of atherosclerosis: an up-date. N. Engl. J. Med. 314: 488 (1986)

89.     Pandolfi, A.: Increased plasma levels of PDGF activity in patients with progressive systemic sclerosis. Proc. Soc. Exp. Biol. Med. 191: 1 (1989)

90.     Huang, J.S., Huang, S.S., Deuel, T.F.: Transforming protein of simian sarcoma virus stimulates autocrine growth of SSV-transformed cells through PDGF cell-surface receptors. Cell 39: 79 (1984)

91.     Heldin, C.-H., Betsholtz, C., Claesson-Welsh, L., Westermark, B.: Subversion of growth regulatory pathways in malignant transformation. Biochim. Biophys. Acta 907: 219 (1987)

92.     Sporn, M.B., Roberts, A.B.: Autocrine growth factors and cancer. Nature 313: 745 (1985)

93.     Travali, S., Koniecki, J., Petralra, S., Baserga, R.: Oncogenes in growth and development. FASEB J., 4: 3209 (1990)

94.     Antoniades, H.N., Pantazis, P., Owen, A.J.: Human platelet-derived growth factor and the sis/PDGF-2 gene. In: Oncogenes, Genes and Growth Factors (G. Guroff, ed.), p. 1, J. Wiley, New York, 1987

95.     Cohen, S.: Isolation of a mouse submaxillary gland protein accelerating incisor eruption and eyelid opening in new-born animal. J. Biol. Chem. 237: 1555 (1962)

96.     Savage, C.R., Inagami, T., Cohen, S.: The primary structure of epidermal growth factor. J. Biol. Chem. 247: 7612 (1972)

97.     Carpenter, G., Cohen, S.: Epidermal growth factor. Annu. Rev. Biochem. 48: 193 (1979)

98.     Downward, J., Yarden, Y., Mayes, E., Scrace, G., Totty, N., Stockwell, P., Ullrich, A., Schlessinger, J., Waterfield, M.D.: Close similarity of epidermal growth factor receptor and verb-B oncogene protein sequences. Nature 307: 521 (1984)

99.     Gill, G.N., Weber, W.: Purification of functionally active epidermal growth factor receptor protein using a competitive antagonist monoclonal antibody and competitive elution with epidermal growth factor. Meth. Enzymol. 146: 82 (1987)

100.   Cohen, S., Carpenter, G.: Human epidermal growth factor: Isolation and chemical and biological properties. Proc. Natl. Acad Sci. USA, 72: 1317 (1975)

101.   Carpenter, G.: Epidermal growth factor. In: Tissue Growth Factors (R. Baserga, ed),p. 89, Springer-Verlag, New York, 1981

102.   Oka, Y., Orth, D.N.: Human plasma epidermal growth factor-β-urogastrone is associated with blood platelets. J. Clin, Invest. 72: 249 (1983)

103.   Hirata, Y., Orth, D.N.: Conversion of high molecular weight human epidermal growth factor (hEGFJ)/urogastrone (UG) to small molecular weight hEGF/UG by mouse EGF-associated arginine esterase. J. Clin. Encocrin. Metabol. 49: 481 (1979)

104.   Heldin, C.-H., Wasteson, A., Westermark, B.: Partial purification and characterization of platelet factors stimulating the multiplication of normal human glial cells. Exp. Cell Res. 109: 429 (1977)

105.   Froesch, E.R., Schmid, C., Schwander, J., Zapf, J.: Actions of insulin-like growth factors. Annu. Rev. Physiol. 47: 443 (1985)

106.   Rinderknecht, E., Humbel, R.E.: Polypeptides with non- suppressible insulin-like and cell-growth promoting activities in human serum: isolation, chemical characterization and some biological properties of forms I and II. Proc. Natl. Acad. Sci USA 73: 2365 (1976)

107.   Rinderknecht, E., Humbel, R.E.: Primary structure of human insulin-like growth factor II. FEBS Lett. 89: 283 (1978)

108.   Zapf, J., Froesch, E.R., Humbel, R.E.: The insulin-like growth factors (IGF) of human serum: chemical and biological characterization and aspects of their possible physiological role. Curr. Top. Cell Regul. 19: 257 (1981)

109.   Hey, A.W., Christopher, A.B., Simpson, R.J., Thorburn, G.D.: Simultaneous isolation of insulin-like growth factors I and II from adult sheep serum. Biochim. Biophys. Acta 997: 27 (1989)

110.   Clemmons, D.R., Van Wyk, J.J.: Somatomedin: Physiological control and effect on cell proliferation. In: Tissue Growth Factors (R. Baserga, ed.), p. 161, Springer-Verlag, Berlin, 1981

111. Massagug, J., Czech, M.P.: The subunit structures of two distinct receptors for insulin-like growth factors I and II and their relationship to the insulin receptor. J. Biol. Chem. 257: 5038 (1982)

112.   Casella, S.J., Han, U.K., D’ Ercole, A.J., Svoboda, M.E., Van Wyk, J.J.: Insulin-like growth factor II binding to the type I sometomedin receptor. Evidence for two high affinity binding sites. J. Biol. Chem. 261: 9268 (1986)

113.   Karey, K.P., Marquardt, H., Sirbasku, D.A.: Human platelet-derived mitogens. I. Identification of insulin like growth factors I and II by purification and N2-amino acid sequence analysis. Blood 74: 1084 (1989)

114.   Varma, K.G., Niewiarowski, S., Holt, J.C., Rucinski, B., Paul, D.: Human platelet basic protein. Its relation to low affinity platelet factor 4 and p-thromboglobulin. Biochim. Biophys. Acta 701: 7 (1982)

115.   Castor, C.W., Miller, J.W., Walz, D.A.: Structural and biological characteristics of connective tissue activating peptide (CTAP-III), a major human platelet-derived growth factor. Proc. Natl. Acad. Sci. USA 80: 765 (1983)

116.   Mullenbach, G.T., Tabrizi, A., Blacher, R.W., Steimer, K.S.: Chemical synthesis and expression in yeast of a gene encoding connective tissue activating peptide-III. A novel approach for the facile assembly of a gene encoding a human platelet-derived mitogen. J. Biol. Chem. 261: 719 (1986)

117.   Miyazono, Κ., Okabe, T., Urabe, A., Takaku, F., Heldin, C.-H.: Purification and properties of an endothelial cell growth factor from human platelets. J. Biol. Chem. 262: 4098 (1987)

118.   Miyazono, Κ., Heldin, C.-H.: High-yield purification of platelet derived endothelial cell growth factor: Structural characterization and establishment of a specific antiserum. Biochemistry 28: 1704 (1989)

119.   Miyazono, Κ., Takaku, F.: Platelet-derived growth factors. Blood Reviews, 3: 269 (1989)

120.   Ishikawa, F., Miyazono, Κ., Heilman, V., Drexler, H., Wernstedt, C., Hagiwara, Κ., Usuki, Κ., Takaku, F., Risau, W., Heldin, C.-H.: Identification of angiogenic activity and the cloning and expression of platelet-derived entothelial cell growth factor. Nature, 338: 557 (1989)

121.   Clemmons, D.R., Isley, W.L, Todd Brown, M.: Dialyzable factor in human serum of platelet origin stimulates endothelial cell replication and growth. Proc. Natl. Acad. Sci. USA 80: 1641 (1983)

122.   King, G.L, Buchwald, S.: Characterization and partial purification of an endothelial cell growth factor from human platelets. J. Clin. Invest. 73: 392 (1984)

123.   Sipes, N.J., Meyskens, F.L Jr., Bregman, M.D.: Biological characterization of an acid-sensitive growth factor from human platelets: Role in the proliferation of human melanoma and bovine endothelial cells. Biochem. Biophys. Res. Commun. 138: 795 (1986)

124.   Sipes. N.J., Bregman, M.D., Meyskens, F.L. Jr.: Stimulation of human metastatic melanoma colony- forming cells by an acid-sensitive factor in human platelet sonicate. Cancer Res. 45: 6268 (1985)

125.   Todd Brown, M„ Clemmons, D.R.: Platelets contain a peptide inhibitor of endothelial cell replication and growth. Proc. Natl. Acad. Sci. USA 83: 3321 (1986)

126.   Huang, W., Kimura, T., Mashima, K. Miyazaki, K., Masaki, H., Yamashita, J., Horio, T.: Purification and properties of epithelial growth inhibitor (EGI) from human platelets: Its separation from type ß transforming growth factor (TGF-ß). J. Biochem. 100: 687 (1986)

127.   Lyons, R.M., Moses, H.I.: Transforming growth factors and the regulation of cell proliferation. Eur. J. Biochem. 187: 467 (1990)

128.   Sporn, M.B., Roberts, A.B., Wakefield, L.M., de Crombrugghe, B.: Some recent advances in the chemistry and biology of transforming growth factor-ß. J. Cell Biol. 105: 1039 (1987)

129.   Roberts, A.B., Sporn, M.B.: Transforming growth factor ß. Adv. in Cane. Res. 51: 107 (1988)

130.   De Larco, J.E., Todaro, G.J.: Growth factors from murine sarcoma virus-transformed cells. Proc. Natl. Acad. Sci. USA. 76: 4001 (1978)

131.   Anzano, M.A., Roberts, A.B., Meyers, C.A., Komoriya, A., Lamb, L.C., Smith, J.M., Sporn, M.B.: Synergistic interaction of two classes of transforming growth factors from murine sarcoma cells. Cancer Res 42: 4776 (1982)

132.   Roberts, A.B., Frolik, C.A., Anzano, M.A., Sporn, M.B.: Transforming growth factors from neoplastic and non-neoplastic tissues. Fed. Prod. 42: 2621 (1983)

133.   Coffey, R.J. Jr., Derynck, R., Wilcox, J.N., Bring man, T.S., Goustin, A.S., Moses, H.L., Pittelkow, M.R.: Production and auto-induction of transforming growth factor-α in human keratinocytes. Nature 328: 817 (1987)

134.   Kondaich, P., Sands, M.J., Smith, J.M., Fields, A., Roberts, A., Sporn, M., Melton, D.: Identification of a novel transforming growth factor-ß (TGF-ß5) mRNA in Xenopus laevis. J. Biol. Chem. 265: 1089 (1990)

135.   Assoian, R.K., Komoriya, A., Meyers, C.A., Miller, D.M., Sporn, M.B.: Transforming growth factor-ß in human platelets. Identification of a major site, purification and characterization. J. Biol. Chem. 258: 7155 (1983)

136.   Van den Eijnden – van Raaij, A.J.M., Koornneef, I., van Zoekn, E.J.J.: A new method for high yield purification of type beta transforming growth factor from human platelets. Biochem. Biophys. Res. Comm. 157: 16 (1988)

137.   Cone, J.L., Brown, D.R., De Larco, J.E.: An improved method of purification of transforming growth factor type ß from platelets. Anal. Biochem. 168: 71 (1988)

138.   Derynck, R., Jarrett, J.A., Chen, E.V., Eaton, D.H., Bell, J.R., Assoian, R.K., Roberts, A.B., Sporn, M.B., Goeddei, D.V.: Human transforming growth factor-ß complementary DNA sequence and expression in normal and transformed cells. Nature 316: 701 (1985)

139.   Nilsen-Hamilton, M.: Transforming growth factor-ß and its action on cellular growth and differentiation. In: Current topics in Developmental Biology vol. 24, Growth Factors and Development, (M. Nilsen-Hamilton, ed.), p. 95, Academic Press, New York, 1990

140.   Madisen, L., Lioubin, M., Farrand, A.M., Brunner, A.M., Purchio, A.F.: Analysis of proteolytic cleavage of recombinant TGF-ß: Production of hybrid molecules with increased processing efficiency. Ann. N.Y. Acad. Sci. USA. 593: 7 (1990)

141.   Pircher, R., Jullien, P., Lawrence, D.A.: ß-Transforming growth factor is stored in human blood platelets as a latent high molecular weight complex. Biochem. Biophys. Res. Commun. 136: 30 (1986)

142.   Miyazono. K., Heilman, K., Wernstedt, C., Heldin, C.-H.: Latent high molecular weight complex of transforming growth factor ß. Purification from human platelets and structural characterization. J. Biol. Chem. 263: 6407 (1988)

143.   Wakefield, L.M., Smith, P.M., Flanders, K.C, Sporn, M.B.: Latent transforming growth factor-ß from human platelets. A high molecular weight complex containing precursor sequences. J. Biol. Chem. 263: 7646 (1988)

144.   Miyazono, K., Yuki, K., Takaku, F., Weinsted, C., Kanraki, T., Olofsson, A., Heilman, U., Heldin, C.-H.: Latent forms of TGF-ß: Structure and Biology. Ann. N.Y. Acad. Sei. N.Y.. 593: 51 (1990)

145.   Kanzakt, I., Olofsson, A. Moren, A., Wernstedt, C., Heilman, U., Miyazono, K., Claesson-Welsh, L., Heldin, C.-H.: Transforming growth factor-ß, binding protein: A component of the large latent complex of TGF-ß, with multiple repeat sequences. Cell 61: 1051 (1990)

146.   Gentry, L.E., Webb, N.R., Lim, G.J., Brunner, A.M., Ranchalis, J.E., Twardzik, D.R., Lioubin, M.N., Marcquardt, H., Purchio, A.F.: Type 1 transforming growth factor-ß: amplified expression of mature and precursor polypeptides in Chinese hamster ovary cells. Mol. Cell Biol. 7: 3418 (1987)

147.   Miyazono, Κ., Heldin, C.-H.: Role for carbohydrate structure in TGF-ß, latency. Nature 338: 158 (1989)

148.   Lyons, R.M., Keski-Oja, J., Moses, H.L.: Proteolytic activation of latent transforming growth factor-ß from fibroblast-conditioned medium. J. Cell Biol. 106: 1659 (1988)

149.   Wakefield, L.M., Thompson, N.L., Flanders, K.C., O’Connor – McCourt, M.D., Sporn, M.B.: Transforming growth factor-ß: Multifunctional regulator of cell growth and phenotype. Ann. N.Y. Acad. Sci. N.Y., 551: 290 (1988)

150.   Cheifetz, S., Weatherbee, J. A., Tsang, M.L-S., Anderson, J.K., Mole, J.E., Lucas, R., Massague, J.: The transforming growth factor-ß system, a complex pattern of cross-reactive ligands and receptors. Cell 48: 409 (1987)

151.   Cheifetz, S., Hernandez, H., Laiho, M., Ten Dijke, P., Iwata, K.K., Massague, J.: Distinct transforming growth factor-ß (TGF-ß) receptor subsets as determinants of cellular responsiveness to three TGF-ß isoforms. J. Biol. Chem. 265: 20533 (1990)

152.   Boyd, F.T., Massague, J.: Transforming growth factor inhibition of epithelial cell proliferation linked to the expression of a 53-kDa membrane receptor. J. BioI. Chem. 264: 2272 (1989)

153.   Seganini, P.R., Seyedin, S.M.: The high molecular weight receptor to transforming growth factor-β contains glycosaminoglycan chains. J. Biol. Chem. 263: 8366 (1988)

154^-Andres, J.L., Stanley, K., Cheifetz, S., Massagui, J.: Membrane-anchored and soluble froms of betaglycan, a polymorphic proteoglycan that binds transforming growth factor-p. J. Cell Biol. 109: 3137 (1990)

155.   Wakefield, L.M., Smith, D.M., Masui, T., Harris, C.C., Sporn, M.B.: Distribution and modulation of the cellular receptor for transforming growth factor-beta. J. Cell Biol. 105: 965 (1987)

156.   Cheifetz, S., Ling, N., GuiElemin, R., Massague, J.: A surface component on GH3 pituitary cells that recognizes transforming growth factor-P, activin and inhibin. J. Biol. Chem. 263: 17225 (1988)

157.   Laiho, M„ Weis, F.M.B., Massague. J.: Concomitant loss of transforming growth factor (TGF)-p receptor types I and II in TGF-P resistant cell mutants implicates both receptor types in signal transduction. J. Biol. Chem. 265: 18518 (1990)

158.   Howe, P.H., Leof, E.B.: Transforming growth factor-β,-treatment of AKR-2B cells is coupled through a pertussis-toxin-sensitive G-protein(s). Biochem. J. 261: 879 (1989)

159.   Roberts, A.B., Anzano, M.A.; Wakefield, L.M., Roche, M.S., Stern, D.F., Sporn, M.B.: Type β-transforming growth factor: A bifunctional regulator of cellular growth. Proc. Natl. Acad. Sci. USA 82: 119 (1985)

160.   Roberts, A.B., Roche, N.S., Sporn, M.B.: Selective inhibition of the anchorage independent growth of myctransfected fibroblasts by retinoic acid. Nature 315: 257 (1985)

161.   Hill, D.J., Strain, A.J., Elstow, S.F., Swenne, I., Milner, R.D.: Bi-functional action of transforming growth factor-β on DNA synthesis in early passage human fetal fibroblasts. J. Cell Physiol. 128: 322 (1986)

162.   Stathakos, D„ Kletsas, D.: Action of human serum growth factors on homologous embryonic lung fibroblasts. Rev. Roum. Biochim. 28: in press (1991)

163.   Centrella, M., McCarthy, T.L., Canalis, E.: Transforming growth factor p is a bifunctional regulator of replication and collagen synthesis in osteoblast enriched cell cultures from fetal rat bone. J. Biol. Chem. 262: 2869 (1987)

164.   Shipley, G.D., Tucker, R.F., Moses, H.L.: Type p-transforming growth factor/growth inhibitor stimulates entry of monolayer cultures of AKR-2B cells into S phase after a prolonged prereplicative interval. Proc. Natl. Acad. Sci. USA 82: 4147 (1985)

165.   Gronwald, R.G.K., Seifert, R.A., Bowen-Pope, D.F.: Differential regulation of expression of two platelet derived growth factor receptors by transforming growth factor-β J. Biol. Chem. 264: 8120 (1989)

166.   Robey, P.G., Young, M.F., Flanders, K.C., Roche, N.S., Kondaiah, P., Harri Reddi, A., Termine, J.D., Sporn, M.B., Roberts, A.B.: Osteoblasts synthesize and respond to transforming growth factor type ß (TGF-p) in vitro. J. Cell Biol. 105: 457 (1987)

167.   Myoken, Y., Kan, M., Sato, G.H., McKeehan, W., Sato, J.D.: Bifunctional effect of transforming growth factor-p (TGF-ß) on endothelial cell growth correlate with phenotypes of TGF-β binding sites. Exp. Cell Res. 191: 299 (1990)

168.   Massagué, J., Kelly, B., Mottola, .C: Stimulation by insulin-like growth factors is required for cellular transformation by type P transforming growth factor. J. Biol. Chem. 260: 4551 (1985)

169.   Masui, T., Wakefield, L.M., Lechner, J.F., La Veck, M.A., Sporn, M.B., Harris, C.C.: Type p transforming growth factor is the primary differentiation inducing serum factor for normal human bronchial epithelial cells. Proc. Natl. Acad. Sci. USA 83: 2438 (1986)

170.   Shipley, G.D., Attelkow, M.R., Wille, J.J., Scott, R.E., Moses, H.L.: Reversible inhibition of normal human prokeratinocyte proliferation by type p transforming growth factor-growth inhibitor in serum free medium. Cancer Res 46: 2068 (1986)

171.   Bryckaert, M.C., Lindroth, M., Lönn, A., Tobelem, G., Wasteson, A.: Transforming growth factor (TGF-p) decreases the proliferation of human bone marrow fibroblasts by inhibiting the platelet derived growth factor (PDGF) binding. Exp. Cell Res. 179: 311 (1988)

172.   Chambárd, J.C., Pouyssegur, J.: TGF-ß inhibits growth factor-induced DNA synthesis in hamster fibroblasts without affecting the early mitogenic events. J. Cell Physiol. 135: 101 (1988)

173.   Baird, A., Durkin, T.: Inhibition of endothelial cell proliferation by type ß-transforming growth factor: Interactions with acidic and basic fibroblast growth factors. Bioch. Biophys. Res. Commun. 138: 476 (1986)

174.   Frater-Schröder, M., Müller, G., Birchmeier, W., Böhlen, P.: Transforming growth factor-beta inhibits endothelial cells proliferation. Bioch. Biophys. Res. Commun. 137: 295 (1986)

175.   Coffey, R.J., Sipes, N.J., Bascom, C.G., Graves Deal R., Pennington, C.Y., Weissman, B.E., Moses, H.L.: Growth modulation of mouse keratinocytes by transforming growth factors. Cancer Res. 48: 1596 (1988)

176.   Ristow, H.J.: BSC-1 growth inhibitor/type β-transforming growth factor is a strong inhibitor of thymocyte proliferation. Proc. Natl. Acad. Sci. USA 83: 5531 (1986)

177.   Hayashi, T., Carr, B.I.: DNA synthesis in rat hepatocytes: inhibition by a platelet factor and stimulation by an endogenous factor. J. Cell Physiol. 125: 82 (1985)

178.   Ishibashi, T., Miller, S., Burstein, A.S.: Transforming growth factor-ß is a potent inhibitor of murine megakaryocytopoiesis in vitro. Blood 69: 1737 (1987)

179.   Anzano, M.A., Roberts. A.B., Sporn, M.B.: Anchorage independent growth of primary rat embryo cells is induced by platelet-derived growth factor and inhibited by type ß transforming growth factor. J. Cell Physiol. 126: 312 (1986)

180.   Fine, A., Goldstein, R.H.: The effect of transforming growth factor-β on cell proliferation and collagen formation by lung fibroblasts. J. Biol. Chem. 262: 3897 (1987)

181.   Kehrl, J.H., Roberts, A.B., Wakefield, L.M., Jakowlev, S., Sporn, M.B., Fouei, A.S.: Transforming growth factor-p is an important immunomodulatory protein for human B-lymphocytes. J. of Immunol. 137: 3855 (1986)

182.   Moses, H.L, Branum, E.L, Proper, J.A., Robinson, R.A.: Transforming growth factor production by chemically transformed cells. Cancer Res. 41: 2842 (1981)

183.   Roberts, A.B., Anzano, M.A., Lamb, L.C., Smith, J.M., Sporn, M.B.: New class of transforming growth factors potentiated by epidermal growth factor: Isolation from non-neoplastic tissues. Proc. Natl. Acad. Sci. USA 78: 5339 (1981)

184.   Raghow, R., Postlethwaite, A.E., Keski-Oja, J., Moses, H.L., Kang, A.H.: TGF-β increases steady state levels of type I procollagen and fibronectin messenger RNAs posttranscriptionally in cultured human dermal fibroblast. J. C/in. invest. 79: 1285 (1987)

185.   Ignotz, R.A., Endo, T., Massagu6, J.: Regulation of fibronectin and type I collagen mRNA levels by transforming growth factor-p. J. Biol. Chem. 262: 6443 (1987)

186.   Ignotz, R.A., Massagu6, J.: Cell adhesion protein receptors as targets for transforming growth factor-p action. Cell 51: 189 (1987)

187.   Edwards, D.R., Murphy, G., Reynolds, J.J., Whitham, S.E., Docherty, A.J., Anglel, P., Heath, J.K.: Transforming growth factor-P modulates the expression of collagenase and metalloproteinase inhibitor. EMBO J. 6: 1809 (1987)

188.   Matrisian, LM„ Leroy, P., Ruhlmann, C., Gesnel. M.C., Breathnach, R.: Isolation of the oncogene and epidermal growth factor-induced transin gene: Complex control in rat fibroblasts. Mot. Cell Biol. 6: 1679 (1986)

189.   Laiho, M., Saksela, Ο., Andreasen, P.A., Keski-Oja, J.: Enhanced production and extracellular deposition of the endothelial-type plasminogen activator inhibitor in cultured human lung fibroblasts by transforming growth factor-p. J. Cell Biol. 103: 2403 (1986)

190.   Nugent, M.A., Newman, M.J.: Inhibition of normal rat kidney cell growth by transforming growth factor-p is mediated by collagen. J. Biol. Chem. 264: 18060 (1989)

191.   Leof, E.B., Proper, J.A., Goustin, A.S., Shipley, G.D., Di Corleto, P.E., Moses, H.L.: Induction of c-sis mRNA and activity similar to platelet-derived growth factor by transforming growth factor p: A proposed model for indirect mitogenesis involving autocrine activity. Proc. Natl. Acad. Sci. USA 83: 2453 (1986)

192.   Ishikawa, O., Le Roy E.C., Trojanowska, M.: Mitogenic effect of transforming growth factor β1 on human fibroblasts involves the induction of platelet-derived growth factor a receptors. J. Cell Physiol. 145: 181 (1990)

193.   Kletsas, D., Stathakos, D.: Action of platelet-derived growth factor (PDGF) and transforming growth factor p (TGF-P) on human embryonic lung fibroblasts in homologous plasma. Hell. Biochem. Biophys. Soc. Newsletter 32: 12 (1991)

194.   Pardee, A.B.: G1 events and regulation of cell proliferation. Science 246: 603 (1989)

195.   Campisi, J., Morreo, G., Pardee, A.B.: Kinetics of Gt transit following brief starvation for serum factors. Exp. Cell Res. 152: 459 (1984)

196.   Larrson, O., Zetterberg, A., Engstrom, N.: Consequences of parental exposure to serum-free medium for progeny cell division. J. Cell Sci. 75: 259 (1985)

197.   Wharton, W., van Wyk, J.J., Ptedjer, W.J.: Inhibition of Balb/c-3T3 cells in late G,: Commitment to DNA synthesis controlled by somatomedin C. J. Cell Physiol. 107: 31 (1981)

198.   Scher, C.D., Shepard, R.C., Antoniades, H.N., Stiles, C.D.: Platelet-derived growth factor and the regulation of the mammalian fibroblasts cell cycle. Biochim. Biophys. Acta 560: 217 (1979)

199.   Yen, A., Pardee, A.B.: Exponential 3T3 cells escape in Mid-G1 from their high serum requirement. Exp. Cell Res. 116: 103 (1978)

200.   Temin, H.M.: Stimulation by serum of multiplication of stationary chicken cells. J. Cell Physiol. 78: 161 (1971)

201.   Stiles, C.D., Isberg, R.R., Pledger, W.J., Antoniades, H.N., Scher, C.D.: Control of Balb/C-3T3 cell cycle by nutrients and serum factors: Analysis using platelet- derived growth factor and platelet-poor plasma. J. Cell Physiol. 99: 395 (1979)

202.   Rossow, P.W., Riddle, V.G.H., Pardee, A.B.: Synthesis of labile, serum dependent protein in early G1 controls animal cell growth. Proc. Natl. Acad. Sci. USA 76: 4446 (1979)

203.   Harrington, M.A., Pledger, W.J.: Characterization of growth factor-modulated events regulating cell proliferation. Meth. Enzymoi. 147: 400 (1987)

204.   Goustin, A.S., Nuttall, G.A., Leof, E.B., Ranganathan, G., Moses, H.L.: Transforming growth factor-p can act as a potent competence factor on AKR-2B cells. Exp. Cell Res. 172: 293 (1987)

205.   Pledger, W.J., Stiles, C.D., Antoniades, H.N., Scher, C.D.: An ordered sequence of events is required before Balb/c-3T3 cells become committed to DNA synthesis. Proc. Natl. Acad. Sci USA 75: 2839 (1978)

206.   Pledger, W.J., Stiles, C.D., Antoniades, H.N., Scher, C.D.: Induction of DNA synthesis in Balb/C-3T3 cells by serum components: reevaluation of the commitment process. Proc. Natf. Acad. Sci. USA 74: 4481 (1977)

207.   Leof, E.B., Wharton, W., van Wyk, J.J., Pledger, W.J.: Epidermal growth factor and somatomedin C regulate G, expression in component Balb/c-3T3 cells. Exp. Cell Res. 141: 107 (1982)

208.   Todaro, G.J., Green, H.: Quantitative studies of the growth of mouse embryo cells in culture and their development into established lines. J. Cell Biol. 17: 299 (1963)

209.   Heldin, C.-H., Wasteson, A., Westermark, B.: Growth of normal human glial ceil in a defined medium containing platelet-derived growth factor. Proc. Natl. Acad Sci. USA 77: 6611 (1980)

210.   Phillips, P.D., Kazuhiko, K., Cristofalo, V.J.: Progressive loss of the proliferative response of senescing WI-38 cells to platelet-derived growth factor, epidermal growth factor, insulin, transferrin and dexamethasone. J. Gerontol. 39: 11 (1984)

211.   Millis, A.J.I., Hoyle, M., Field, B.: Human fibroblasts conditioned media contains growth-promoting activities for low density cells. J. Cell Physiol. 93: 17 (1977)

212.   Atkinson, P.R., Bala, R.M.: Partial characterization of a mitogenic factor with somatomedin-like activity produced by cultured WI-38 human fibroblasts. J. Cell Physiol. 107: 317 (1981)

213.   Clemmons, D.R., van Wyk, J.J.: Evidence for a functional role of endogenously produced somatomedinlike peptides in the regulation of DNA synthesis in cultured human fibroblasts and porcine smooth muscle cells. J. Clin. Invest. 75: 1914 (1985)

214.   Clemmons, D.R., Shaw, D.S.: Purification and biological properties of fibroblast somatomedin. J. Biol. Chem. 261: 10293 (1986)

215.   Clemmons, D.R.: Age dependent production of a com-petence factor by human fibroblasts. J. Cell. Physiol. 114: 61 (1983)

216.   Westermark, B., Heldin, C.-H., Ek, B„ Johnsson, A., Mellström, Κ., Nister, M., Wasteson, A.: Biochemistry and biology of platelet-derived growth factor. In: Growth and Maturation Factors (G. Guroff, ed,), vol. 1, p. 73, Wiley, New York, 1983

217.   Scher, C.D., Pledger, W.J., Martin, P., Antoniades, H.N., Stiles, C.D.: Transforming viruses directly reduce the cellular growth requirement for a platelet derived growth factor. J. Cell Physiol. 97: 371 (1978)

218.   Chen, Y., Rabinovitch, P.S.: Mitogen response and cell cycle kinetics of Swiss 3T3 cells in defined medium: Differences from human fibroblasts and effects of cell density. Exp. Cell Res. 190: 145 (1990)

219.   Böhmer, R.M., Forsberg, Κ., Westermark, B.: Growth inhibition of mitogen-stimulated fibroblasts induced by double-stranded RNA depends on cell density. Exp. Cell Res. 191: 115 (1990)

220.   Massaguö, J.: The transforming growth factor-ß family. Annu. Rev. Cell Biol. 6: 597 (1990)

221.   Palladino, M.A., Morris, R.E., Starnes, H.F., Levinson, A.D.: The transforming growth factor-betas. A new family of immunoregulatory molecules. Ann. N.Y. Acad Sci. 593: 181 (1990)

222.   Wahl, S.M., McCartney-Francis, N., Allen, J.B., Dougherty, E.B, Dougherty, S.F.: Macrophage production of TGF-ß and regulation by TGF-ß. Ann. N.Y. Acad. Sci. 593: 188 (1990)

223.   Umeno, Y., Okuda, A., Kimura, G.: Proliferative behaviour of fibroblasts in plasma-rich culture medium. J. Cell Sci. 94: 567 (1989)

224.   Morgan, C.J., Pledger, W.J.: Cell cycle dependent growth factor regulation of gene expression. J. Cell Physiol. 141: 535 (1982)

225.   Kawahara, R.S., Deuel, T.F.: Platelet-derived growth factor-inducible gene JE is a member of a family of small inducible genes related to platelet factor 4. J. Biol. Chem. 264: 679 (1989)

226.   Drucker, B.J., Manon, H.J., Roberts, T.M.: Oncogenes, growth factors and signal transduction. N. Engl. J. Med. 321: 1383 (1989)

227.   Dvorak, H.F.: Tumors: Wounds that do not heal. Similarities between tumor stroma generation and wound healing. N.    Engl. J. Med. 315: 1650 (1986)

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