Τόμος 9 (1995) – Τεύχος 1 – Άρθρο 4 – Επιθεώρηση Κλινικής Φαρμακολογίας και Φαρμακοκινητικής-Διεθνής Έκδοση – Volume 9 (1995) – Issue 1 – Article 4 – Epitheorese Klinikes Farmakologias και Farmakokinetikes-International Edition

By | | Άρθρο επισκόπησης - Review Article, Πλήρες Κείμενο στα Αγγλικά - Full Text in English, Πρόδρομος Β. Ισόπουλος - Prodromos B. Issopoulos

 

Title Medicinal plants in the district of Epirus (Greece) II: analytical investigations of ursolic acid using four different tetrazolium salts
Author Prodromos B. Issopoulos

Laboratory of Inorganic and Pharmaceutical Analytical Chemistry, Department of Chemistry, University of Ioannina, GR-45110 Ioannina, Greece
Citation Issopoulos, P.B.: Medicinal plants in the district of Epirus (Greece). II: analytical investigations of ursolic acid using four different tetrazolium salts, Epitheorese Klin. Farmakol. Farmakokinet. 9(1): 32-38 (1995)
Publication Date Accepted for publication: 1 April 1995
Full Text Language English
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Keywords Spectrophotometric microdetermination, ursolic acid, tetrazolium salts, medicinal plants.
Other Terms review article
Summary A new spectrophotometric method for the determination of micromolar concentrations of ursolic acid (URSA) in pure form is described. The proposed method is performed using one out of four different tetrazolium salts, such as tetrazolium blue chloride, TLBC (I), 2,3,5-triphenyitatrazolium chloride, TPTC (II), neotetrazolium chloride, NTZC (III), and tetrazolium violet chloride, TZVC (IV). The method is based on the reduction of the tetrazolium salt used by URSA, in an alkaline ethanolic medium. The formozans exhibit, just in case, an absorption maximum (λmax), an apparent molar absorptivity (logε) and a Sandeill’s sensitivity (in ng/cm), such as: 525 nm (4.29; 28.9 for I), 485 nm (3.43; 168.7 for II), 507 nm (4.18; 30.1 for III) and 512 nm (3.41; 173.5 for IV). Beer’s law is obeyed over the range of 1.0-50.0 μg/ml (for I and III) and 5.0-150.0 μg/ml (for II and IV), while the optimum concentration range is 2.5-35.0 μg/ml (for I and III) and 12.5- 125.0 μg/ml (for II and IV). The regression line equations, as well as the correlation coefficient (r) for n = 18 determinations were calculated as: A=3.48*10-2C – 5.17*10-3 (r – 0.9999) for I; A=5.89*10-3C + 2.02*10-3 (r = 1.0000) for II; A=3.33*10-2C – 2.61*10-3 (r = 1.0000) for III and A=5.83*10-3C – 3.85*10-3 (r = 0.9998) for IV. The accuracy and the precision of the new analytical method were experimentally checked and considered as very satisfactory.
References 1.         Issopoulos P.B.: Medicinal plants in the district of Epirus (Greece). I: Isolation of ursolic acid from leaves and flowers of Rosemary (Rosemarinus officinalis L.) and study of its physicochemical, analytical and medicinal properties. Epitheor. Klin. Farmakol. Farmakokinet. Int. Ed. 8: 62-72 (1994)

2.        Dong X., Tang Y., Yang M.: Colorimetric assay of ursolic acid tablets. Yaowu Fenxi Zazhi 5: 45-46 (1985); Chem. Abstr. 102: 172726g (1985)

3.        Popova O.I., Muraveva D.A.: Ursolic acid from leafy shoots of Viscum album, Khi. Prir. Soedin 3: 412-413 (1990); Chem. Abstr. 113: 208421x (1990)

4.        Jia Y., Yan X., Nie K.: Comparative analysis of ursolic acid in Crataegus pinnatifida Bge. var. major N.E.Br. before and after processing. Zhongguo Zhongyao Zazhi 14: 466-468 (1989); Chem. Abstr. 113: 25750p (1990)

5.        Akhmedkodzhaeva N.M., Svechnikova A.N., Igol’nihova O.P.: Evaluation of quality of dosage forms from Crataegus fruits. Farm. Zh. (Kiev) 2: 58- 62 (1990); Chem. Abstr. 113: 84930r (1990)

6.        Hao R.X., Yan C.P., Sun X.F., Wang H.: Analysis of ursolic acid in Crataegus pinnatifida Bge var. major N.E.Br, by HPLC. Yaowu Fenxi Zazhi 14: 30- 32 (1994); Anal. Abstr. 56: 10G155 (1994)

7.         Lu S.H., Wang Q., Xia G.C., Hou Y.L.: Determination of oleanolic acid and ursolic acid in Ligustrum lucidum Alt. by HPLC. Yaowu Fenxi Zazhi 13: 291- 294 (1993); Anal. Abstr 56: 5G178 (1994)

8.        Issopoulos P.B.: Sensitive colorimetric assay of carbidopa and methyldopa using tétrazolium blue chloride in pharmaceutical products. Pharm. Weekbl. Sci. Ed. 11: 213-217 (1989)

9.        Issopoulos P.B.: Analytical investigations of isonicotinic acid hydrazide (isoniazid). Ill: Colorimetric determination of microquantities of isoniazid using tetrazolium blue chloride. Acta Pharm. Hung. 61: 198- 204 (1991)

10.      Issopoulos P.B.: Analytical investigations of isonicotinic add (isoniazid). IV: Colorimetric determination of microquantities of isoniazid using 2,3,5-triphenyltetrazolium chloride. Acta Pharm. Jugosi. 41: 123-128 (1991)

11.       Issopoulos P.B., Economou P.T.: Analytical in-vestigations of isonicotinic acid hydrazide (isoniazid). VII: Spectrophotometric determination of microquantities of isoniazid in pharmaceutical formulations using two different tétrazolium salts. Analusis 20: 31-35 (1992)

12.      Issopoulos P.B., Economou P.T.: Spectrophotometric method for the determination of carbidopa using neotetrazolium chloride. J. Pharm. Pharmacol. 44: 1020-1022 (1992)

13.      Issopoulos P.B., Economou P.T.: Spectrophotometric determination of micromolar concentrations of a-methyldopa using tetrazolium violet chloride. Indian Drugs 29: 355-359 (1992)

14.      Issopoulos P.B., Economou P.T.: A new high- sensitivity spectrophotometric method for the determination of microconcentrations of amethyldopa. II Farmaco 48: 127-135 (1993)

15.      Görög S., Horvath P.: Analysis of steroids. Part XXXI. Mechanism of the tetrazolium reaction of corticosteroids. Analyst 103: 346-353 (1978)

16.      Graham R.E., Biehl E.R., Kenner C.T.: Effect of solvent on tetrazolium reaction. J. Pharm. Sci. 65: 1048-1053 (1976)

17.       Graham R.E., Biehl E.R., Kenner C.T.: Effect of laboratory light on tétrazolium reaction and on stability of formazans in various solvents. J. Pharm. Sci. 66: 965-970 (1977)
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