| Title | In vitro model for evaluation of essential oils materials phototoxic activity | |
| Authors | Vassiliki Kefala, Ioanna Kostara, Haris Karageorgiou and Dionysios D. Varonos
Department of Experimental Pharmacology, Medical School, University of Athens, 75 Mikras Asias St., Goudi, 115 27 Athens, Greece |
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| Citation | Kefala, V., Kostara, I., Karageorgiou, H., Varonos, D.D.: In vitro model for evaluation of essential oils materials phototoxic activity, Epitheorese Klin. Farmakol. Farmakokinet. 12(3): 53-57 (1998) | |
| Publication Date | Received for publication: 10 February 1998
Accepted for publication: 15 March 1998 |
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| Full Text Language | English | |
| Order – Buy | pharmakonpress[at]pharmakonpress[.]gr | |
| Keywords | Cytotoxicity, guinea pig, rats, phototoxicity, photoirritation, Saccharomyces cerevisiae. | |
| Other Terms | review article | |
| Summary | Phototoxicity or photoirritation has been evaluated in rats and guinea pigs by various methods as a safety test prior to initial exposure of human. These methods, besides the fact that they are expensive, require killing of lots of animals, which is unwanted’ We have modified an assay for evaluation of essential oils for phototoxic potential prior to human testing. Our assay uses Saccharomyces cerevisiae, seeded in an agar overlay on top of a plate count agar base. Activity is quantified by cytotoxicity measured as zone of inhibition. As for the essential oils that we test in vitro, we detect photobiological activity. As positive control we use 8-Methoxypsoralen 18-MOP) at a concentration of 10-2%. The creation of inhibition clear zones of the Saccharomyces cerevisiae development suggest the phototoxic results of the substance we are studying. | |
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