| Title | In vitro model for the evaluation of sunscreen agents phototoxic activity | |
| Authors | Vassiliki Kefala, Ioanna Kostara, Haris Karageorgiou and Dionysios D. Varonos
Department of Experimental Pharmacology, Medical School, University of Athens, 75 Mikras Asias St., Goudi, 115 27 Athens, Greece |
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| Citation | Kefala, V., Kostara, I., Karageorgiou, H. and Varonos, D.D.: In vitro model for the evaluation of sunscreen agents phototoxic activity, Epitheorese Klin. Farmakol. Farmakokinet. 12(3): 59-62 (1998) | |
| Publication Date | Received for publication: 10 February 1998
Accepted for publication: 15 March 1998 |
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| Full Text Language | English | |
| Order – Buy | pharmakonpress[at]pharmakonpress[.]gr | |
| Keywords | Sunscreen agents, phototoxicity, photoirritation, Saccharomyces cerevisiae. | |
| Other Terms | review article | |
| Summary | The phototoxicity and the photoirritation have been defined in the past through various methods of experimentation on animals, in order for some substances to be safely used by humans. The methods requiring the use of animals are disadvantageous, because, on the one hand, they demand the killing of lots of animals and on the other they have a high cost. In our method, we use ultraviolet radiation (UV-A) in order to radiate the substances we are studying during their diffusion from a paper disc placed upon agar freshly seeded with Saccharomyces cerevisiae (Fleischman’s yeast) (1). As positive control we use 8-Methoxypsoralen (8-MOP) at a concentration of 10-2%. The creation of clear zones of inhibition of Saccharomyces cerevisiae yeast development suggests the phototoxic effect of the substance we are studying. Our method is inexpensive, simple and gives results within a short period of time, which is in 61 hours. The correlation with positive human phototoxicity results is excellent, so that the protection of the consumers is successful. | |
| References | 1. Weinberg H., Springer S.T.: The evaluation in vitro of fragrance materials for phototoxic activity, J. Soc. Cosmet. Chem. 32: 303-315 (1981)
2. Urbach F.M.D.: Sources of radiant energy for medical uses. J. Ιnu. Derm. 32: 167-169 (1959) 3. Daniels F.Jr.: A simple microbiological method for demonstrating photo toxic compounds. J. Invest. Dermatol. 44: 259- 263 (1965) 4. Bjellerup M., Moller H.: Photoaugmentation in drug phototoxicity. J. Invest. Dermatol. 75: 228-229 (1980) 5. Harber L.C., Baer R.L.: Pathogenic mechanisms of drug-induced photosensitivity. J. Invest. Dermatol. 58: 327-342 (1972) 6. Maureen B.R, Fitzpatrick M.D.: The biologic actions of solar radiation on skin with a note on sunscreens. J. Inv. Surg. Oncol. 3: 199-204 (1977) 7. Tenenbaum S., DiNardo J., Morris W.E., Wolf B.A., Schnetzinger R.W.: A quantitative in vitro assay for the evaluation phototoxic potential of topically applied materials. Cell. Biol. Toxicol. 1: 1 (1984) 8. Klain K.: Encyclopedia of UV absorbency for sunscreen products. Cosm. Toilet. 107: 53-62 (1992) 9. DiNardo J., Wolf B.A.: A quantitative in vitro assay for the evaluation of phototoxic potential of topically applied materials. II Modification and assessment of low level activity. J. Soc. Cosmet. Chem. 36: 425-433 (1985) 10. Macleod T.M., Frain-Bell W.: The study of the efficacy of some agents used for protection of the skin from exposure to light. Br. J. Derm. 84: 266 (1971) 11. Zimmerman M.C.: Ultraviolet light therapy. Arch. Derm. 78: 646-652 (1958) |
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