| Title | In vitro effects of trypsin, chymotrypsin and papain on the histopathology of guinea pig skin | |
| Authors | Evangelia E. Protopapa¹, Alexandra Tsigonia-Primikiriou¹ and Nicolaos G. Stavrianeas²
1. Technological Educational Institution of Athens, Egaleo, Athens 2. Dermatological Clinic, Medical School of the University of Athens, Athens, Greece |
|
| Citation | Protopapa, E.E., Tsigonia-Primikiriou, A., Stavrianeas, N.G.: In vitro effects of trypsin, chymotrypsin and papain on the histopathology of guinea pig skin, Epitheorese Klin. Farmakol. Farmakokinet. 8(3): 134-136 (1994) | |
| Publication Date | Received for publication: 10 November 1994
Accepted for publication: 10 December 1994 |
|
| Full Text Language | English | |
| Order – Buy | Ηλεκτρονική Μορφή: pdf (10 €) – Digital Type: pdf (10 €)
pharmakonpress[at]pharmakonpress[.]gr |
|
| Keywords | Enzymes, papain, chymotrypsin, trypsin, skin, hair follicle, guinea pig, epilation, histology. | |
| Other Terms | review article | |
| Summary | We have evaluated, at the histological level, the in vitro effects of three proteolytic enzymes, trypsin, chymotrypsin and papain on guinea pig skin. After mechanical epilation with wax, pieces of guinea pig skin were soaked in 1mg/ml enzyme solutions at 37 °C for different time periods, subsequently embedded in paraffin, cut and the sections stained with hematoxylin-eosin. All three enzymes led to intense histological effects such as the presence of epithelial cells with hyperchromatic nuclei in the center of the shaft of the hair follicles, detachment of the keratin layer from the granular layer and acanthotytic changes of the skin. Trypsin, in addition, leads to pemphygous like defects at the borderline of the epithelium. Chymotrypsin shows the most intense effects among the three enzymes. The observed reported in vivo action of the three enzymes, support their application as biological epilatory agents. | |
| References | 1. Protopapa, E.E., Tsigonia-Primikiriou, A., and Stavrianeas, N.G.: Effects of proteolytic enzymes papain, chymotrypsin and trypsin on the guinea pig skin, with special reference to the hair follicle. Epith. Klin. Farmakol. Farmakokinet. Int.. Ed. 8: 84 (1994) | |
| Relative Papers |
Online ISSN 1011-6575
Άρθρα Δημοσιευμένα σε αυτό το Περιοδικό Καταχωρούνται στα:
- Chemical Abstracts
- Elsevier’s Bibliographic Databases: Scopus, EMBASE, EMBiology, Elsevier BIOBASE SCImago Journal and Country Rank Factor
Articles published in this Journal are Indexed or Abstracted in: • Chemical Abstracts • Elsevier’s Bibliographic Databases: Scopus, EMBASE, EMBiology, Elsevier BIOBASE SCImago Journal and Country Rank Factor
Συντακτικη Επιτροπή-Editorial Board
| ΕΤΗΣΙΑ ΣΥΝΔΡΟΜΗ 1994 – ANNUAL SUBSCRIPTION 1994 | |
| Γλώσσα Πλήρους Κειμένου – Full Text Language | Αγγλικά, Γαλλικά – English, French |
| Παραγγελία – Αγορά – Order – Buy | Ηλεκτρονική Μορφή: pdf (70 €) – Digital Type: pdf (70 €)
pharmakonpress[at]pharmakonpress[.]gr |
| Έντυπη Μορφή (70 € + έξοδα αποστολής) – Printed Type (70 € + shipping)
pharmakonpress[at]pharmakonpress[.]gr |
|