| Title | Yeasts produce secondary metabolites with hypolipemic activity | |
| Authors | A. Cimerman and N. Gunde-Cimerman
National Institute of Chemistry, 61115 Ljubljana, Hajdrihova 19, Slovenia |
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| Citation | Cimerman, A., Gunde-Cimerman, N.: Yeasts produce secondary metabolites with hypolipemic activity, Epitheorese Klin. Farmakol. Farmakokinet. 11(2-3): 88-90 (1997) | |
| Publication Date | 1997 | |
| Full Text Language | English | |
| Order – Buy | pharmakonpress[at]pharmakonpress[.]gr | |
| Keywords | Isolations, HKG CoA reductase, imhibitors. | |
| Other Terms | review article | |
| Summary | During the second phase of our research work we wanted to test different yeast strains of the Culture Collection of the Institute of Chemistry (MZKI) for their ability to produce HMG CoA reductase inhibitors. After growing those on beer wort and potato dextrose agar slants the yeasts were cultivated in a two-step fermentation procedure in a substrate sustaining the production of inhibitors (1). The vegetative phase of the fermentation lasted 2 days and the production 1-5 days. AH experiments were carried out on a rotary shaker at 30 °C (200 rpm). After fermentation, the fermentation broth was extracted with methanol, filtered, converted to a sodium salt and lactonized (2). The extracts were initially analysed by TZC (3), and followed by HPLC analyses (4). Lovastatin was used as a standard in a tablet formulation (Mevacor -Merck). The isolated compounds were further analysed by mass spectrometry and the spectra compared with the standard sample of lovastatin. The isolated com-pounds of the two strains of Candida cariosilignicola and Pichia labacensis were tested also by means of a biological test using HMG CoA reductase from CHO-K1 cells (5,6). | |
| References | 1. Gunde-Cimerman N., Plemenitas A., Cimerman A.: A hydroxymethylglutaryl-CoA reductase inhibitor synthesized by yeasts. FEMS Microbiology Letters, 132: 39-43 (1995)
2. Endo, A. (1981) HMG CoA reductase inhibitors from Peniciilium and Monascus sp. Meth. Enzymol. 72: 684-689 (1981) 3. Gunde-Cimerman N., Friedrich J., Cimerman A., Benicki N.: Screening fungi for the production of an inhibitor of HMG CoA reductase: Production of mevinolin by the fungi of the genus Pleurotus. FEMS Microbiol. Lett. 111: 203-206 (1993) 4. Monaghan R.L., Alberts A.W., Hoffman C.H., Albers- Schonberg G.: (1980) Hypochoiesteroiemic fermentation products and process of preparation. U.S. patent 4, 231, 938, 1980 5. Gunde-Cimerman N., Plemenitas A., Cimerman A.: Pleurotus fungi produce mevinolin, an inhibitor of HMG CoA reductase. FEMS Microbiol. Lett. 113: 333-338 (1993) 6. Plemenitas A., Havel C.M., Watson J.A.: Sterolmediated regulation of mevalonic acid synthesis. J. Biol. Chem. 265: 17012-17017 (1990) 7. Gunde-Cimerman N., Cimerman A.: Pleurotus fruiting bodies contain the inhibitor of HMG CoA reductase. Exp. Mycol. 19: 1-6 (1995) 8. Lee J.D., Komagata K.: Pichia cellobiosa, Candida cari- osilignicola and Candida succiphila, new species of methanol-assimilating yeasts. Int. J. Syst. Bacteriol. 30: 514-519 (1980) 9. Friedrich J., Cimerman A., Susa L.: Strokovno srecanje Biotechnologrja, Meeting Abstracts, Ljubljana, p. 129 (1983) 10. Cimerman A., Kockova-Kratochvilova A.: Pichia labacensis n.sp. Z. Allg. Mikrobiol. 24: 219-222 (1984) |
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Online ISSN 1011-6575
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