Τόμος 13 (1999) – Τεύχος 1 – Άρθρο 5 – Επιθεώρηση Κλινικής Φαρμακολογίας και Φαρμακοκινητικής-Διεθνής Έκδοση – Volume 13 (1999) – Issue 1 – Article 5 – Epitheorese Klinikes Farmakologias και Farmakokinetikes-International Edition

Title Nucleotidyl-tyrosine and nucleotidyl-peptides containing tyrosine hydrolysis by various enzymes, separation and characterization by HPLC
Authors M. Liakopoulou-Kyriakides1, C.A. Tsoleridis2, A.A. Pantazaki2, and A. Metaxas1

1. Department of Chemical Engineering, Section of Chemistry,

2. Department of Chemistry, Section of Organic Chemistry and Biochemistry, Aristotle University of Thessaloniki, Thessaloniki, 54006, GREECE

Citation Liakopoulou-Kyriakides, M., Tsoleridis, C.A., Pantazaki, A.A., Metaxas, A.: Nucleotidyl-tyrosine and nucleotidyl-peptides containing tyrosine hydrolysis by various enzymes, separation and characterization by HPLC, Epitheorese Klin. Farmakol. Farmakokinet. 13(1): 43-48 (1999)
Publication Date Accepted for publication: 17 June 1999
Full Text Language English
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Keywords Nucleotides-tyrosine, nucleotides-peptides, esterases, phosphatases, HPLC.
Other Terms review article
Summary A series of derivatives of tyrosine and peptides containing tyrosine with uridine-5′-monophosphate and thymidine-5′-monophosphate, through the functional hydroxyl group of tyrosine, were synthesized by the dicyclo-hexylcarbodiimide method in pyridine at 35-40 °C. The effect of various esterases on the stability of the phosphoester bond was investigated. The products were purified and characterized by HPLC and/or other spectroscopic techniques.
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10.            Robins M.J., Simon L.N., Stout M.E., Ivanovich G.A., Schweizer M.P., Rousseau R.S., Robins R.K.: Nucleoside peptides. I The synthesis of 5′-deoxy-5′-amino-5′-N-ami- noacyl peptide derivatives of guanosine, adenosine and 2′- deoxyadenosine and their effect on cell-free protein synthesis. J. Am. Chem. Soc. 93: 4387-4392 (1971)

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13.            Juodka B.A., Kirreliene V.A., Porilinis P.S.: Oligonu-cleotides and nucleotide-peptides. Synthesis and properties of nucleotidyl(P-O)amino acids (peptides) and their esters. Nucleosides and Nucleotides, 1(2): 112-125 (1982)

14.            Schattenkerk C., Wreesmaan C.T.J., de Graaf M.J., van der Marel G.A., van Boom J.H.: Synthesis of a naturally occuring nucleopeptide fragment via a phosphotriester approach. Tetrahedron Letters 45: 5197-5200 (1984)

15.            Kuyl-Yeheskiely E., Tromp C.M., Schaeffer A.H., van der Marel G.A., van Boom J.H.: A model study directed towards the preparation of nucleopeptides via H-phosphonate intermediates. Nucl. Acids Res. 15: 1807-1817 (1987)

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