Τόμος 14 (2000) – Τεύχος 1 – Άρθρο 6 – Επιθεώρηση Κλινικής Φαρμακολογίας και Φαρμακοκινητικής-Διεθνής Έκδοση – Volume 14 (2000) – Issue 1 – Article 6 – Epitheorese Klinikes Farmakologias και Farmakokinetikes-International Edition

Title Medicinal plants in the district of Epirus (Greece) V: hypericin, a pharmacologically active ingredient of hypericum perforatum L

Author

Prodromos B. Issopoulos

Laboratory of inorganic and Pharmaceutical Analytical Chemistry, Department of Chemistry, University of Ioannina, GR-451 10 Ioannina, Hellas (Greece)

Citation Issopoulos, P.B.: Medicinal plants in the district of Epirus (Greece) V: hypericin, a pharmacologically active ingredient of hypericum perforatum L, Epitheorese Klin. Farmakol. Farmakokinet. 14(1): 32-42 (2000)
Publication Date Received for publication: 20 October 1999

Accepted for publication: 10 November 1999

Full Text Language English
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Keywords Medicinal plants, Greece, Epirus, hypericin.
Other Terms review article
Summary The experimental part of the present paper begins with the successful application of the Soxhlet’s technique, an outdated but at the same time simple, rapid and efficient method for the extraction of Hypericin (HPCN) from the medicinal herb Hypericum perforatum L (Common St John’s wort, HPPF) and then a study of the physicochemical properties (spectrophotometric: λ1 max = 590 nm and λ2 max=550 nm; spectrofluorometric: λex = 470 nm and λem = 585 nm; TLC: Rf (HPCN): 0.60 and Rf (ψ-HPCN): 0.64) in order to determine exactly the identity, the purity and the concentration of the resulted ethanolic extracts was performed. Finally, the paper is concluded by a detailed quotation and discussion on the plurality and the versatility of the therapeutic applications of the examined herb (HPPF), and its pharmacologically active ingredient (HPCN), on a wide spectrum of diseases of various etiology and gravity.
References 1.    Anonymous: In: Hagers Handbuch der Pharmazeutischen Praxis. Pp. 214-219, Springer-Verlag, Fünfter Band, Berlin, 1976

2.    Brockmann H., Lindemann E., Ritter K-H., Depke F.: Uber Oxy-Deriyate des Helianthrens und meso-Naphthodianthrens; zur Konstitution des Hypericins. ΙΙΙ. Mitteilung: Chem. Ber. 61: 583-593 (1950)

3.    Brockmann H., Falkenhausen E.H.F., Dorlars A.: Die Konstitution des Hypericins. Naturwiss 37: 540 (1950)

4.    Brockmann H., Kluge F.: Zur Synthese des Hypericins. Naturwiss. 38: 141 (1951)

5.    Brockmann H., Falkenhausen E.H.F., Neeff R., Dorlars A., Budde G.: Die Konstitution des Hypericins. Chem. Ber. 64: 865-889 (1951)

6.    Brockmann H., Muxfeldt H.: Die Synthese des Hypericins. Naturwiss. 40: 411 (1953)

7.    Brockmann H., Kluge F., Muxfeldt H.: Totalsynthese des Hypericins. Chem. Ber. 90: 2302-2318(1957)

8.    Bianchιni F., Fantano A.C.: In: Guide to Plants and Flowers (Simon & Schuster, eds), p. 361, New York, 1974

9.    Liebes L., Mazur Y., Freeman D., Lavie D., Lavie G., Kudler N., Mentoza S., Levin B., Höchster H., Meruelo D.: A method for the quantitation of Hypericin, an antiviral agent, in biological fluids by HPLC. Anal. Biochem. 195: 77-85 (1991)

10.  Nafis M., Jardon P.: Propriétés spectroscopiques et photophysiques de complexes métalliques de l’hypericine en relation avec leur activité photodynamique. J. Chim. Phys. 91: 99-112 (1994)

11.  Micali G., Lanuzza F., Curro P.: HPLC-determination of the biologically active principle hypericin in phytotherapeutic vegetable extracts and alcoholic beverages. J. Chromatogr. 731: 336-339 (1996)

12.  Kartnig Th., Göbel L: Determination of Hypericin and Pseudohypericin by thin-layer chromatography-densitometry. J. Chromatogr. 609: 423-426 (1992)

13.  Φωκάς Γ.: Ειδική Φαρμακογνωσία. Σελ. 281,283, 284, Θεσσαλονίκη, 1975

14.  Λίντσκι Μ.Ι.: Φαρμακοθεραπεία των Εσωτερικών Παθήσεων. Εκδ. Γρ,Κ, Παρισιάνος, σελ. 324, 474, 483, Αθήνα, 1959

15.  Hoffmann D.: Η Νέα Ολιστική Βοτανοθεραπεία. Εκδ. Διόπτρα, σελ. 417, Αθήνα, 1997

16.  Launert E.: In: The Hamlyn Guide to Edible & Medicinal Plants of Britain and Northern Europe. The Hamlyn Publishing Group Ltd., pp. 40-41, London, 1989

17.  Tonero A.: Therapeutic product for the treatment of several diseases, such as rheumatism. Belg. Patent 654,916, 16.02.1965, Chem. Abstr. 92, 759-761, 1966)

18.  Hadjur C., Richard M.J., Parat M.O., Jardon P., Favier A.: Photodynamic effects of Hypericin on lipid peroxidation and antioxidant status in melanoma cells. Photochem. Photobio. 64: 375-381 (1996)

19.  Couldwell W.T..: Hypericin: a potential antiglioma therapy. Neurosurgery 35: 705-710 (1994)

20.  Moraleda G., Wu T.T., Jilbert A.R. et al.: Inhibition of duck Hepatitis В virus replication by Hypericin. Antiviral. Res. 20: 235-247 (1993)

21.  Degar S., Lavie G., Meruelo D.: Photodynamic inactivation of radiation Leucemia virus produced from Hypericin-treated cells. Virology 197: 796-800 (1993)

22.  Cohen P.A., Hudson J.B., Towers G.H.: Antiviral activities of Anthraquinones, Bianthrones and Hypericin derivatives from Lichens. Experientia 52: 180-183 (1996)

23.  Kraus G.A., Pratt D., Tossberg J., Carpenter S.: Antiretroviral activity of synthetic Hypericin and related analogs. Biochem. Biophys. Res. Commun. 172: 149-153 (1990)

24.  Takahashi O., Nakanishi S., Kobayashi E., Nakano H., Suzuki K., Tamaoki T.: Hypericin and Pseudohypericin specifically inhibit Protein kinase C possible relation to their antiretroviral activity. Biochem, Biophys. Res. Commun. 65: 1207-1212(1989)

25.  Lavie G., Valentine F., Levin B., et al.: Studies of the mechanisms of action of the antiretroviral agents Hypericin and Pseudohypericin. Proc. Natl. Acad. Sci. USA 86: 5963- 5967 (1989)

26.  Meruelo D., Lavie G., Lavie D.: Therapeutic agents with dramatic antiretroviral activity and little toxicity at effective doses: aromatic polycyclic diones Hypericin and Pseudohypericin. Proc. Natl. Acad. Sci. USA 85: 5230-5234 (1988)

27.  Nishiuchi T., Utsumi T., Kanno T., et al.: Inhibition of neutrophil superoxide generation by Hypericin, an antiretroviral agent. Arch .Biochem. Biophys. 323: 335-342 (1995)

28.  Utsumi T., Okuma M., Kanno T., et al.: Light-dependent inhibition of Protein kinase C and superoxide generation of neutrophils by Hypericin, an antiretroviral agent. Arch. Biochem. Biophys. 316: 493-497 (1995)

29.  Okpanyi S.N., Lidzba H., Scholl B.C., Miltenburger H.G.: Genotoxicity of a standardized Hypericum extract. Arzneimitteiforchung 40: 851-855 (1990)

30.  Neuman,H.: Skin food with new active substances. Selfen-Ole-Fette-Wachae 92: 759-761 (1966)

31.  Sharikova K.K., Garagulya A.D., Khazanovich R.L.: Antimicrobial properties of some species of St.John’s wort cultivated in Uzbekistan. Mikrobiol. Zh. 32: 494-497 (1970)

32.  Omel’chuk-Myakushko T.Ya., Fastovskaya A.Ya.: Dynamics of the accumulation of antimicrobic substances in Hypericum perforatum. Rast. Resur.: 346-349 (1968)

33.  Lui H., Anderson R.R.: Photodynamic therapy in Dermatology: recent developments. Dermatol. Clin. 11: 1- 13(1993)

34.  Agostinis P., Vandenbogaerde A., Donella-Deana A., et al.: Photosensitized inhibition of growth factor-regulated Protein kinases by Hypericin. Biochem. Pharmacol. 49: 1615-1622 (1995)

35.  Agostinis P., Donella-Deana A., Cuveele J., et al.: A comparative analysts of the photosensitized inhibition of growth-factor regulated Protein kinases by Hypericin-derivatives. Biochem. Biophys, Res. Commun. 220: 613- 617 (1996)

36.  Evstifeeva T.A., Sibîriak S.V.: The immunotropic properties of biologically active products obtained from Klamath weed (Hypericum perforatum L). Eksp. Klin. Farmakol. 59: 51-54(1996)

37.  Harris M.S., Sakamoto T., Kimura K, et al.: Hypericin inhibits cell growth and induces apoptosis in retinal pigment epithelial cells: possible involvement of Protein kinase C. Curr, Eye Res 15: 255-262 (1996)

38.  Miskovsky P., Sureau F., Chinsky L, Turpin P.Y.: Subcellular distribution of Hypericin in human cancer cells. Photochem. Photobiol. 62: 546-540(1995)

39.  Zhang W., Lawa R.E., Hintona D.R., Su Y., Couldweell W.T.: Growth inhibition and apoptosis in human neuroblastoma SK-N-SH-cells, induced by Hypericin, a potent inhibitor of Protein kinase C. Cancer Lett. 96: 31-35 (1995)

40.  Jarvis W.D., Turner A.J., Povirk L.F., Traylor R.S., Grant S.: induction of apoptotic DNA fragmentation and cell death in HL-60 human promyelocytic leucemia cells by pharmacological inhibitors of Protein kinase C. Cancer Res. 54: 1707-1714(1994)

41.  Gopalakrishna R., Chen Z.H., Gundimeda U.: Tobacco smoke tumor promoters, catechol and hydroquinone, induce oxidative regulation of Protein kinase C and influence invasion and metastasis of lung carcinoma cells. Proc. Natl. Acad. Sci. USA 91: 12233-12237 (1994)

42.  VanderWerf Q.M., Saxton R.E., Chang A., et al.: Hypericin: a new laser phototargeting agent for human cancer cells. Laryngoscope 106: 479-483 (1996)

43.  Vandenbogaerde A.L., Geboes K.R., Cuveele J.R, Agostinis P.M., Merievede W.J., de Witte P.A.: Antitumor activity of photosensitized Hypericin on A431 cell xenografts. Anticancer Res. 16: 1619-1625(1996)

43a. Vandenbogaerde A.L, Cuveele J.F., Proot P., Him- pens B.E., Merievede W.J., de Witte, P.A. J. Photochem. Photobiol. B. 38: 136-142 (1997)

43b. Vandenbogaerde A.L., Delaey E.M., Vantieghem A.M., Himpens B.E., Merievede W J., de Witte P.A.: Photochem. Photobiol. 67: 119-125 (1998)

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