| Title | Testosterone decreases rat liver and intestinal epithelium apoptosis | |
| Authors | H. Frangou and S. Massouridou
Department of General Biology, Medical School, Aristotle University, Thessaloniki, Greece |
|
| Citation | Frangou, H., Masouridou, S.: Testosterone decreases rat liver and intestinal epithelium apoptosis, Epitheorese Klin. Farmakol. Farmakokinet. 21(3): 257-260 (2007) | |
| Publication Date | Accepted for publication (Final version): September 10, 2007 | |
| Full Text Language | English | |
| Order – Buy | Ηλεκτρονική Μορφή: pdf (10 €) – Digital Type: pdf (10 €)
pharmakonpress[at]pharmakonpress[.]gr |
|
| Keywords | Testosterone, rat liver, intestinal epithelium, caspase-3, apoptosis. | |
| Other Terms | review article | |
| Summary | Increased levels of testosterone have been associated with programmed cell death in many tissues. The biological effects of testosterone depend upon the tissue on which it acts and upon the various stages of life. The aim of this study is to examine whether testosterone following administration at different doses and ages of animals influences apoptotic process in rat liver and intestinal epithelium and to elucidate the mechanisms of its action, including caspase-3. Male rats (Wistar) were divided into age groups A (3 months), and B the oldest (9 months). They were further divided into subgroups: A1 and B1 subgroups constituted the controls; A2, B2 subgroups were received at once intramuscular injection of testosterone (2.5 mg/kg b.w), and A3, B3 subgroups received testosterone injection (5 mg/kg b.w.). The animals were sacrificed 5 days later, livers removed and after appropriate manipulation they were homogenized with a Potter Elvehjem homogenator. Caspase-3 activity was determined, using a colorimetric assay (kit, Sigma), based on the hydrolysis of the peptide substrate acetyl-Asp-Glu-Val-Asp p-Nitroaniline (pNA) moiety, according to the method of Nicholson with minor modification. The caspase 3 activity was calculated in µmol pNA released per min per ml. Our findings showed decreased caspase-3 activity in intestinal epithelium as well as in liver after treatment with testosterone, which was independent of dosage and age. Enhanced caspase-3 activity was observed in liver when rats were treated with the highest dose only. We assume that testosterone can restrict apoptotic death in rat intestinal epithelium and hepatocytes. | |
| References | 1. Hsieh J.T., Lin S.H.: Andogen regulation of cell adhesion molecule gene expression in rat prostate during organ degeneration. C-CAM belongs to a class of androgen-repressed genes associated with enriched stem/ amplifying cell population after prolonged castration. J. Biol. Chem. 269: 3711-6 (1994)
2. Wilson J.W., Potten C.S.: Cell turnover: Intestine and other tissues. In: When cells die II (Lockshin R.A., Zakeri Z., eds). Pp 201-240. John Wiley & Sons, Inc., New Jersey, 2004 3. Wikman A., Karlsson J., Carlstedt I., Artursson P. Artursson. Pharm. Res. 10: 843-852 (1993) 4. Snyder P.J. Androgens. 1635-1648. In: Goodman & Gilman· s The pharmacological basis of therapeutics. Hardman JG., Limbird LE., Gilman AG., (eds). 10th ed. International edition, London, (2001) 5. Green M.D., Oturu E.M., Temhly T.R. Stable expression of a human liver UDP-glucuronosyltransferase (UGT2B15) with activity toward steroid and xenobioticsubstrates. Drug Metab Dispos 22: 7999-805 (1994) 6. Cheng Z., Rios G.R., King C.D., Coffman B.L., Green M.D., Moiarrabi B., Mackenzie P.I., Tephly T.R. Glucuronidation of catechol estrogens by expressed human UDP-glucuronosyltransferases (UGTs) 1A1, 1A3, and 2B7. Toxicol Sci 45(1):52-7 (1998) 7. Gall W.E., Zawada G., Mojarrabi B., Tephly T.R., Green M.D., Coffman B.L., Mackenzie P.I., Radominska-Pandya A. Differential glucuronidation of bile acids, androgens and estrogens by the humanrecombinant UDP- glucuronosyltransferases UGTIA3 and 2B7. J Steroid Biochem Mol Biol 70:101-108 (1999) 8. Zaitoun A.A.., Apelqvist G., Al-Mardini H., Gray T., Bengtsson F., Record C.O. Quantitative studies of liver atrophy after portacaval shunt in the rat. J Surg Res. 131(2):225-32 (2006) 9. Nickolson D.W., Ali A., Thorrberry N.A., Vaillancourt J.P., Ding C.K., Gallant M., Gareau Y., Griffin P.R., La- belle M., Lazebnik Y.A., Munday N.A., Raju S.M., Smulson M.E.,Yamin T-T, Yu V.L., Miller D.K. Identification and inhibition of the ICE/CED-3 protease necessary for mammalian apoptosis. Nature 376: 37-43 (1995) 10. Sakahira H, Enari M, Nagata S. Cleavage of CAD inhibitor in CAD activation and DNA degradation during apoptosis. Nature, 1998, 391: 96-99. 11. Kamada S., Kusano H., Fujita H., Ohtsu M., Koya R.C., Kuzamaki N., Tsujimoto Y. A cloning method for caspase substrates that uses the yeast two-hybrid system: cloning of the antiapoptotic gene gelsolin. Proc. Natl. Acad. Sci. USA, 95:8532-8537 (1998) 12. Cohen G.M. Caspases: the executioners of apoptosis. Biochem. J. 326: 1-16 1997) 13. Sohlenius-Sternbeck A.-K., Orzechowski A. Characterization of the rates of testosterone metabolism to various products and of glutathione transferase and sulfotransferase activities in rat intestine and comparison to the corresponding hepatic and renal drug-metabolizing enzymes. Chemico-Biological Interactions 148:49-56 (2004) 14. Zhang Q.Y., Wikoff J., Dunbar D., Kaminsky L. Characterization of rat small intestinal cytochrome P450 composition and inducibility. Drug Metab. Dispos. 24: 322-328 (1996) 15. Groener J.E., Macdonald I. Lipid response to female gonadal hormones of female rats fed a high glucose or a high fructose diet. Nutr Metab. 19(3-4): 212-22 (1975) 16. Matsumoto A.M. The testis. In: Endocrinology & Metabolism 4th ed., pp 635-705 Felig P., Frohman L.A. (eds), McGRAW-HILL, Inc., New York, 2001 17. Berzola D., Gandolfo M.T., Salvatore F., Vilaggio B., Gianiorio F., Traverso P., Deferrari G., Garibotto G. Testosterone promotes apoptotic damage in human renal tubular cells. Kidney Int. 65(4): 1252-61 (2004) 18. Wunderlich F., Dkhil M.A., Mehnert L.I., Braun J.V., El-Khadragy M., Borsch E., Hermsen D., Benten W.P., Pfeffer K., Mossmann H., Krucken J. Testosterone responsiveness of spleen and liver in female lymphotoxin beta receptor-deficient mice resistant to blood-stage malaria. Microbes Infect. 7(3):399-409 (2005) |
|
| Relative Papers |
Online ISSN 1011-6575
Άρθρα Δημοσιευμένα σε αυτό το Περιοδικό Καταχωρούνται στα:
- Chemical Abstracts
- Elsevier’s Bibliographic Databases: Scopus, EMBASE, EMBiology, Elsevier BIOBASE SCImago Journal and Country Rank Factor
Articles published in this Journal are Indexed or Abstracted in: • Chemical Abstracts • Elsevier’s Bibliographic Databases: Scopus, EMBASE, EMBiology, Elsevier BIOBASE SCImago Journal and Country Rank Factor
Συντακτικη Επιτροπή-Editorial Board
| ΕΤΗΣΙΑ ΣΥΝΔΡΟΜΗ 2007 – ANNUAL SUBSCRIPTION 2007 | |
| Γλώσσα Πλήρους Κειμένου – Full Text Language | Αγγλικά – English |
| Παραγγελία – Αγορά – Order – Buy | Ηλεκτρονική Μορφή: pdf (70 €) – Digital Type: pdf (70 €)
pharmakonpress[at]pharmakonpress[.]gr |
| Έντυπη Μορφή (70 € + έξοδα αποστολής) – Printed Type (70 € + shipping)
pharmakonpress[at]pharmakonpress[.]gr |
|