| Title | Lack of evidence for TNF-α gene expression in mechanically exposed canine pulp | |
| Authors | Andreas B. Kokkas1, Antonis Goulas2, Serafim Papadimitriou³, Basil Kokkas2 and Dimitrios Tziafas1
1. Department of Endodontology, School of Dentistry 2. Department of Pharmacology, School of Medicine 3. Companion Animal Clinic, School of Veterinary Medicine, Aristotle University of Thessaloniki, Thessaloniki, Hellas |
|
| Citation | Kokkas, A.B., Goulas, A., Papadimitriou, S., Kokkas, B., Tziafas, D.: Lack of evidence for TNF-gene expression in mechanically exposed canine pulp, Epitheorese Klin. Farmakol. Farmakokinet. 23(2): 89-91 (2009) | |
| Publication Date | Accepted for publication (Final Version): July 1, 2009 | |
| Full Text Language | English | |
| Order – Buy | Ηλεκτρονική Μορφή: pdf (10 €) – Digital Type: pdf (10 €)
pharmakonpress[at]pharmakonpress[.]gr |
|
| Keywords | Pulp exposure, dog, TNF-α, gene expression. | |
| Other Terms | review article | |
| Summary | Intentional exposure of pulp to the environment of the buccal cavity usually results in inflammation of variable degree. The objective of this study was to test whether the analysis of TNF-α gene expression with RT-PCR can assist in the monitoring of such changes, in canine teeth. Pulp tissue from 24 teeth, from a total of 5 animals, was used. Exposure was effected by preparation of cervical cavities by a round bur and lasted for 2 (n=8) or 7 (n=8) days. Pulp from unopened teeth (n=8) served as the control. TNF-α gene expression was practically undetected in either exposed or unexposed pulp, under the conditions used. We conclude that analysis of TNF-α gene expression with conventional RT-PCR cannot be used as a marker of short-to-medium term pulp tissue changes accompanying intentional pulp exposure in canine teeth. | |
| References | 1. Bergenholts G.: Evidence for bacterial causation of adverse pulpal responses in resin-based dental restorations. Crit. Rev. Oral. Biol. Med. 11: 467-480 (2000)
2. Ozcelik B., Kuraner T., Kendir B., Asan E.: Histopathological evaluation of the dental pulps in crownfractured teeth. J. End. 26: 271-273 (2000) 3. Cox C.F., Bergenholts G., Fitzgerald M., Heys D.R., Avery J.K., Baker J.A.: Capping of the dental pulp mechanically exposed to the oral microflora: a 5 week observation of wound healing in the monkey. J. Oral Pathol. 11: 327-339 (1982) 4. Panagakos F.S., O’Boskey F.J. Jr., Rodriguez E.: Regulation of pulp cell matrix metalloproteinase production by cytokines and lipopolysaccharides. J. Endod. 22: 358-361 (1996) 5. O’Boskey F.J. Jr., Panagakos F.S.: Cytokines stimulate matrix metalloproteinase production by human pulp cells during long-term culture. J. Endod. 24: 7-10 (1998) 6. Ueda L., Matsushima K.: Stimulation of plasminogen activator activity and matrix metalloproteinases of human dental pulp-derived cells by tumor necrosis factor-alpha. J. Endod. 27: 175-179 (2001) 7. Lin S.K., Wang C.C., Huang S., Lee J.J., Chiang C.P., Lan W.H., Hong C.Y.: Induction of dental pulp fibroblast metalloproteinase-1 and tissue inhibitor of metalloproteinase-1 gene by expression interleukin-1 alpha and tumor necrosis factor-alpha through prostaglandin-depended pathway. J. Endod. 27: 185-189 (2001) 8. Rauschenberger C.R., Bailey J.C., Cootauco C.J.: Detection of human IL-2 in normal and inflamed dental pulps. J. Endod. 23: 366-370 (1997) 9. Pezelj-Ribaric S., Anic I., Brekalo I., Miletic I., Hasan M., Simunovic-Soskic M.: Detection of tumor necrosis factor alpha in normal and inflamed human dental pulps. Arch. Med. Res. 33: 482-484 (2002) 10. Zehnder M., Delaleu N., Du Y., Bickel M.: Cytokines mRNA gene expression – part of host defence in pulpitis. Cytokine 22: 84-88 (2003) 11. Piattelli A., Rubini C., Fioroni M., Tripodi D., Strocchi R.: Transforming growth factor-beta 1 (TGF-beta 1) expression in normal healthy pulps and in those with irreversible pulpitis. Int. Endod. J. 37: 114-119 (2004) 12. Kokkas A., Goulas A., Varsamidis K., Mirtsou V., Tziafas D.: Irreversible but not reversible pulpitis is associated with up-regulation of TNF-α gene expression in human pulp. Int. End. J. 40: 198-203 (2007) 13. Isermann G., Kaminski E.J.: Pulpal response to bacteria in the dog. Oral Surg. Oral Med. Oral Pathol. 48: 353-357 (1979) 14. Koliniotou-Koumpia E., Tziafas D.: Pulpal responses following direct pulp capping of healthy dog teeth with dentine adhesive systems. J. Dent. 33: 639-647 (2005) 15. German A.J., Helps C.R., Hall E.J., Day M.J.: Cytokine mRNA expression in mucosal biopsies from German shepherd dogs with small intestinal enteropathies. Dig. Dis. Sci. 45: 7-17 (2000) 16. Tani K., Morimoto M., Hayashi T., Inokuma H., Ohnishi T., Hayashiya S., Nomura T., Une S., Nakaichi M., Taura Y.: Evaluation of cytokine messenger RNA expression in peripheral blood mononuclear cells from dogs with canine demodicosis. J. Vet. Med. Sci. 64: 513-518 (2002) 17. Engel E., Klein R., Baumgartner W., Hoffmann B.: Investigations on the expression of cytokines in the canine corpus luteum in relation to dioestrus. Anim. Reprod. Sci. 87: 163-167 (2005) 18. Harran-Ponce E., Holland R., Barreiro-Lois A., Lopez-Beceiro A.M., Pereira-Espinel J.L.: Consequences of crown fractures with pulpal exposure: histopathological evaluation in dogs. Dent. Traumatol. 18: 196-205 (2002) 19. Kawashima N., Nakano-Kawanishi H., Suzuki N., Tagaki M., Suda H.: Effect of NOS inhibitor on cytokine and COX2 expression in rat pulpitis. J. Dent. Res. 84: 762-767 (2005) |
|
| Relative Papers |
Online ISSN 1011-6575
Άρθρα Δημοσιευμένα σε αυτό το Περιοδικό Καταχωρούνται στα:
- Chemical Abstracts
- Elsevier’s Bibliographic Databases: Scopus, EMBASE, EMBiology, Elsevier BIOBASE SCImago Journal and Country Rank Factor
Articles published in this Journal are Indexed or Abstracted in: • Chemical Abstracts • Elsevier’s Bibliographic Databases: Scopus, EMBASE, EMBiology, Elsevier BIOBASE SCImago Journal and Country Rank Factor
Συντακτικη Επιτροπή-Editorial Board
| ΕΤΗΣΙΑ ΣΥΝΔΡΟΜΗ 2009 – ANNUAL SUBSCRIPTION 2009 | |
| Γλώσσα Πλήρους Κειμένου – Full Text Language | Αγγλικά – English |
| Παραγγελία – Αγορά – Order – Buy | Ηλεκτρονική Μορφή: pdf (70 €) – Digital Type: pdf (70 €)
pharmakonpress[at]pharmakonpress[.]gr |
| Έντυπη Μορφή (70 € + έξοδα αποστολής) – Printed Type (70 € + shipping)
pharmakonpress[at]pharmakonpress[.]gr |
|