| Title | A study on the prevalence of the most common genetic polymorphisms of the cytochromes P450 CYP206, CYP2C9 and CYP3A5 in the Greek population | |
| Authors | Kostas Arvanitidis, Georgia Ragia, Vasilis Tsamadias, Maria Iordanidou, Sofia Kyriaki, Anna Tavridou and Vangelis G. Manolopoulos
Laboratory of Pharmacology, Medical School, Democritus University of Thrace, Alexandroupolis, Greece |
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| Citation | Arvanitidis, K., Ragia, G., Tsamadias, V., Iordanidou, M., Kyriaki, S. et al: A study on the prevalence of the most common genetic polymorphisms of the cytochromes P450 CYP206, CYP2C9 and CYP3A5 in the Greek population, Epitheorese Klin. Farmakol. Farmakokinet. 20(2): 71-74 (2006) | |
| Publication Date | Accepted for publication: 19-20 May 2006 | |
| Full Text Language | English | |
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| Keywords | CYP2D6, CYP2C9, CYP3A5, pharmacogenetics, polymorphism. | |
| Other Terms | review article | |
| Summary | The objective of this study are to determine the prevalence of the most common polymorphisms of the cytochromes P450 (CYP) CYP206, CYP2C9 and CYP3A5 and to predict each genotype frequency in the Greek population. 0NA isolated from peripheral blood samples derived from 200 non-related subjects of Greek origin was used to determine the frequency of allelic variants CYP206*3, CYP206*4, CYP2C9*2, CYP2C9*3 and CYP3A5*3 by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method and CYP206*2 (gene duplications) by long PCR analysis. In the 200 volunteers genotyped for CYP206, CYP2C9, and CYP3A5, the allele frequencies of CYP206*3 and CYP206*4 were 2% and 18.75% respectively. CYP206*2 gene duplication was found in 7.5% of the subjects. For CYP2C9*2 and CYP2C9*3 alleles, the frequencies were 12% and 8.75% respectively. The CYP3A5*3 allele was abundantly present in the Greek population with an allelic frequency of 94.25%. In conclusions, while CYP2C9 and CYP3A5 allelic variants are in accordance, the prevalence of allelic variants and predicted genotypes of CYP206 in the Greek population is moderately increased compared to those reported in other Caucasian European populations. | |
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