| Title | STAT5B forms dynamic complexes with the δ-opioid receptor and selective G protein subunits | |
| Authors | Eirini-Maria Georganta, Adamantia Agalou and Zafiroula Georgoussi
Laboratory of Cellular Signaling and Molecular Pharmacology, Institute of Biology, National Centre for Scientific Research Demokritos, Athens, Greece |
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| Citation | Georganta, E.-M., Agalou, A., Georgoussi, Z.: STAT5B forms dynamic complexes with the δ-opioid receptor and selective G protein subunits, Epitheorese Klin. Farmakol. Farmakokinet. 24(2): 91-94 (2010) | |
| Publication Date | 2010 | |
| Full Text Language | English | |
| Order – Buy | Ηλεκτρονική Μορφή: pdf (10 €) – Digital Type: pdf (10 €)
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| Keywords | Opioid receptor, Signal Transducer and Activator of Transcription, G protein, signaling complex, tyrosine phosphorylation. | |
| Other Terms | Review article | |
| Summary | Recent observations from our laboratory have shown that the Signal Transducer and Activator of Transcription 5A (STAT5A) interacts with the μ-opioid receptor (μ-OR) and is phosphorylated upon μ-OR stimulation. In the present study we demonstrate that another member of the STAT family, STAT5B, interacts directly with the conserved juxtamembrane region of the C-terminal tail of the δ-opioid receptor (δ-CT). Agonist exposure of HEK293 cells, stably expressing the flag-δ-opioid receptor (δ-OR), leads to a G protein-dependent STAT5Β phosphorylation and transcriptional activation mediated by c-Src kinase. Co-immunoprecipitation studies demonstrate that δ-OR serves as a platform for the formation of a multi-component signaling complex signalosome, consisting of STAT5B, c-Src, Gβγ and selective Gα protein subunits. Collectively, our results uncover a novel signaling pathway through which δ-OR might be involved in the transcriptional regulation of STAT5B-dependent genes. | |
| References | 1. Mazarakou G., Georgoussi Z.: STAT5A interacts with and is phosphorylated upon activation of the μ-opioid receptor. J. Neurochem. 93: 918-931 (2005)
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Online ISSN 1011-6575
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Articles published in this Journal are Indexed or Abstracted in: • Chemical Abstracts • Elsevier’s Bibliographic Databases: Scopus, EMBASE, EMBiology, Elsevier BIOBASE SCImago Journal and Country Rank Factor
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