| Title | Application of the genotoxicity assay comet as a test for evaluating occupational exposure to chemotherapeutic agents | ||
| Authors | Ekaterini Kyriakopoulou1, Eleni Georgopoulou2, Christina Emmanouil1, Elizabeth Vagka2, Kyriaki Machera1, Theodoros C. Constantinidis3 and Ekaterini Chatzaki4
1. Laboratory of Pesticides Toxicology, Benaki Phytopathological Institute 2. Program of Postgraduate Studies Health and Safety in Workplaces, Democritus University of Thrace 3. Laboratory of Hygiene and Environmental Protection, Medical School, Democritus University of Thrace 4. Laboratory of Pharmacology, Medical School, Democritus University of Thrace |
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| Citation | Kyriakopoulou, E., Georgopoulou, E., Emmanouil, C., Vagka, E., Machera, K. et al.: Application of the genotoxicity assay comet as a test for evaluating occupational exposure to chemotherapeutic agents, Epitheorese Klin. Farmakol. Farmakokinet. 24(2): 162-164 (2010) | ||
| Publication Date | 2010 | ||
| Full Text Language | English | ||
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| Keywords | Chemotherapy, Greek hospitals, cancer, research. | ||
| Other Terms | Review article | ||
| Summary | The expansion of anti-cancer chemotherapy has led to a significant concern about the health and safety work conditions of the personnel handling these drugs that have been characterized by the International Agency for Research on Cancer as hazardous to human. However, no reliable monitoring test of occupational exposure is currently clinically applied for the medical surveillance of these employees. Based on a descriptive self-evaluation survey, we have previously shown inadequate health and safety design and practices related to chemotherapeutic drug handling in Greek hospitals. In the present study, we use a quantitative and objective genotoxicity method, the comet assay, to evaluate occupational exposure to chemotherapeutics. DNA single strand breaks as revealed by comet in peripheral mononuclear blood cells were significantly higher in a group of employees involved in the preparation, handling or administration of cancer chemotherapeutic agents that in a control group. Comet data were correlated to demographic/epidemiological health data of the subjects as well as to the self-evaluation subjective occupational exposure data. Our results demonstrate clearly genotoxic effects related to occupational exposure to chemotherapeutics, indicating insufficient safety design and practices. They also suggest that SSBs could be an appropriate biomarker for evaluating genotoxicity in these employees. The comet assay in peripheral blood could provide an easy tool of the Health and Safety Department in hospitals for the exposure monitoring. | ||
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Online ISSN 1011-6575
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Articles published in this Journal are Indexed or Abstracted in: • Chemical Abstracts • Elsevier’s Bibliographic Databases: Scopus, EMBASE, EMBiology, Elsevier BIOBASE SCImago Journal and Country Rank Factor
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