| Title | The C-termini of the μ– and δ– opioid receptors differentially bind to Signal Transducers and Activators of Transcription, STAT5A and STAT5B | ||
| Authors | E.-M. Georganta, G. Mazarakou, A. Agalou and Z. Georgoussi
Laboratory of Cellular Signaling and Molecular Pharmacology, Institute of Biology, National Center for Scientific Research Demokritos, Ag. Paraskevi Attikis 153 10 Athens, Greece |
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| Citation | Georganta, E.-M., Mazarakou, G., Agalou, A., Georgoussi, Z.: The C-termini of the μ– and δ– opioid receptors differentially bind to Signal Transducers and Activators of Transcription, STAT5A and STAT5B, Epitheorese Klin. Farmakol. Farmakokinet. 22(2): 152-155 (2008) | ||
| Publication Date | 23-25 May 2008 | ||
| Full Text Language | English | ||
| Order – Buy | Ηλεκτρονική Μορφή: pdf (10 €) – Digital Type: pdf (10 €)
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| Keywords | μ-Opioid receptor, δ-opioid receptor, STAT5A, STAT5Β, signaling complex. | ||
| Other Terms | Review article | ||
| Summary | Signal Transducer and Activator of Transcription (STAT) proteins are transcription factors known to be activated by G protein-coupled receptors including opioid receptors. Recent observations have shown that STAT5A interacts directly with the C-terminal region of the μ-opioid receptor (μ-OR) and is phosphorylated upon receptor stimulation. In this regard, we aimed to determine whether members of the STAT family interact also with the δ-opioid receptor (δ-OR) and whether STAT5 phosphorylation is universal among the opioid receptors. Our results indicate that STAT5A and STAT5B interact with μ-OR and δ-OR respectively, but the strength of association between these interacting partners differs depending on the activation status of the opioid receptors. Opioid receptor stimulation leads to STAT phosphorylation by Src kinase and subsequently phosphorylated STATs activate gene transcription. Comparative analysis of the Gα proteins implicated in the STAT5A/B activation by μ-OR and δ-OR suggests that although endogenous Giα/Goα proteins are involved in δ-OR-activated STAT5Β phosphorylation, STAT5A stimulation by μ-OR is mediated by alternative mechanisms. | ||
| References | 1. Reich N.C.: STAT dynamics. Cytokine Growth Factor Rev. 18: 511-518 (2007)
2. Mazarakou G., Georgoussi Z.: STAT5A interacts with and is phosphorylated upon activation of the mu-opioid receptor. J. Neurochem. 93: 918-931 (2005) 3. Georgoussi Z., Leontiadis L., Mazarakou G., Merkouris M., Hyde K., Hamm H.: Selective interactions between G protein subunits and RGS4 with the C-terminal domains of the μ– and δ-opioid receptors regulate opioid receptor signaling. Cell. Signal. 18: 771-782 (2006) 4. Georgoussi Z.: Novel Interacting partners regulating opioid receptor signaling. In: Molecular Aspects of G Protein-Coupled Receptors (Ciruela F., Lujan R., eds). Chapter 6, Nova Science Publishers Inc., 2007 5. McWhinney C.D., Dostal D., Baker K.: Angiotensin II activates Stat5 through Jak2 kinase in cardiac myocytes. J. Mol. Cell Cardiol. 30: 751-761 (1998) 6, Wu E.H., Lo R.K., Wong Y.H.: Regulation of STAT3 activity by G16-coupled receptors: Biochem. Biophys. Res. Commun. 303: 920-925 (2003) 7. Law P.Y., Wong Y.H., Loh H.H.: Molecular mechanisms and regulation of opioid receptor signaling. Annu. Rev. Pharmacol. Toxicol. 40: 389-430 (2000) 8. Lo R.K., Wong Y.H.: Signal transducer and activator of transcription 3 activation by the δ-opioid receptor via Gα14 involves multiple intermediates. Mol. Pharmacol. 65: 1427-1439 (2004) |
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| Relative Papers | |||
Online ISSN 1011-6575
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Articles published in this Journal are Indexed or Abstracted in: • Chemical Abstracts • Elsevier’s Bibliographic Databases: Scopus, EMBASE, EMBiology, Elsevier BIOBASE SCImago Journal and Country Rank Factor
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