| Title | Developmental heterogeneity in splicing of the postnatal rat retinal N-methyl-D-aspartate glutamate receptor 1 | ||
| Authors | G. Manta1, S. Taraviras2, E. Kouvelas1 and A. Mitsacos1
1. Department of Physiology, and 2. Department of Pharmacology, Faculty of Medicine, University of Patras, Patra, Greece |
||
| Citation | Manta, G., Taraviras, S., Kouvelas, E., Mitsacos, A.: Developmental heterogeneity in splicing of the postnatal rat retinal N-methyl-D-aspartate glutamate receptor 1, Epitheorese Klin. Farmakol. Farmakokinet. 22(2): 235-237 (2008) | ||
| Publication Date | 23-25 May 2008 | ||
| Full Text Language | English | ||
| Order – Buy | Ηλεκτρονική Μορφή: pdf (10 €) – Digital Type: pdf (10 €)
pharmakonpress[at]pharmakonpress[.]gr |
||
| Keywords | NMDA receptor, NR1, development, alternative splicing, visual system. | ||
| Other Terms | Review article | ||
| Summary | The N-methyl-D-aspartate (NMDA) type of glutamate ionotropic receptor is considered to have important roles in neuronal differentiation and synapse consolidation in developing CNS. NMDA receptors assemble as tetramers containing NR1 and NR2A-2D, and occasionally NR3A-3B subunits. The gene encoding the NR1 subunit contains three exons that are alternatively spliced to form eight distinct subunit variants. Knowing that alternative splicing of the NR1subunit offers molecular diversity to the NMDA receptor, we have addressed in the present study the question of whether the alternative splicing of NR1 subunit of the NMDA receptor is regulated during retinal development. We have examined the splice variants of the NR1 subunit (NR1-1, NR1-2, NR1-3 NR1-4, NR1a and NR1b isoforms) in the mammalian rat retina by semi-quantitative PCR during postnatal development. Our results showed that NR1-1 isoform increased gradually from P3 to P35 and then decreased to the initial levels at P60. NR1-2 isoform was expressed at low levels at P3 and P9, increased at P14 with no significant changes thereafter. NR1-3 isoform was expressed at low levels from P3 to P14, increased at P21, remained increased at P35 and decreased at P60. NR1-4 was expressed at low levels at P3 and gradually increased from P9 to P35 and decreased almost to the initial levels at P60. Finally, NR1a and NR1b following a parallel developmental profile showed a significant peak of expression at P35. These results suggest that the NR1 subunit isoforms are differentially regulated and that molecular changes in the NMDA receptor are taking place in rat retina during development. | ||
| References | 1. Watanabe M., Mishina M., Inoue Y.: Differential distributions of the NMDA receptor channel subunit mRNAs in the mouse retina. Brain Res. 634: 328-332 (1994)
2. Gründer T., Kohler K., Kaletta A., Guenther E.: The distribution and developmental regulation of NMDA receptor subunit proteins in the outer and inner retina of the rat. J. Neurobiol. 44: 333-342 (2000) 3. Laurie D.J., Seeburg P.H.: Regional and developmental heterogenity in splicing of the rat brain NMDAR1 mRNA. J. Neurosci. 14: 3180-3194 (1994) 4. Kreutz M.R., Böckers T.M., Bockmann J., Seidenbecher C.I., Kracht B., Vorwerk C.K., Weise J., Sabel B.A.: Axonal injury alters alternative splicing of the retinal NR1 receptor: the preferential expression of the NR1b isoform is crucial for retinal ganglion cell survival. J. Neurosci. 18: 8278-8291 (1998) 5. Logan S.M., Rivera F.E., Leonard J.P.: Protein kinase C modulation of recombinant NMDA receptor currents: roles for the C-terminal C1 exon and calcium ions. J. Neurosci. 19: 974-986 (1999) 6. Zhang Y., Auerbach A.: Kinetic properties of NMDA receptors in embryonic Xenopus spinal neurons. J. Neurophysiol. 74: 153-161 (1995) 7. Tingley W.G., Ehlers M.D., Kameyama K., Doherty C., Ptak J.B., Riley C.T., Huganir R.L.: Characterization of protein kinase A and protein kinase C phosphorylation of the N-Methyl–D-aspartate receptor NR1 subunit using phosphorylation site-specific antibodies. J. Biol. Chem. 272: 5157-5166 (1997) 8. Bisti S., Gargini C., Chalupa L.M.: Blokade of glutamate- mediated activity in the developing retina pertubs the functional segregation of ON and OFF pathways. J. Neurosci. 18: 5019-5025 (1998) 9. Wong W.T., Faulkner-Jones B.E., Sanes J.R., Wong R.O.L.: Rapid dendritic remodeling in the developing retina: dependence on neurotransmission and reciprocal regulation by Rac and Rho. J. Neurosci. 20: 5024-5036 (2000) |
||
| Relative Papers | |||
Online ISSN 1011-6575
Άρθρα Δημοσιευμένα σε αυτό το Περιοδικό Καταχωρούνται στα:
- Chemical Abstracts
- Elsevier’s Bibliographic Databases: Scopus, EMBASE, EMBiology, Elsevier BIOBASE SCImago Journal and Country Rank Factor
Articles published in this Journal are Indexed or Abstracted in: • Chemical Abstracts • Elsevier’s Bibliographic Databases: Scopus, EMBASE, EMBiology, Elsevier BIOBASE SCImago Journal and Country Rank Factor
Συντακτικη Επιτροπή-Editorial Board
| ΕΤΗΣΙΑ ΣΥΝΔΡΟΜΗ 2008 – ANNUAL SUBSCRIPTION 2008 | |
| Γλώσσα Πλήρους Κειμένου – Full Text Language | Αγγλικά – English |
| Παραγγελία – Αγορά – Order – Buy | Ηλεκτρονική Μορφή: pdf (70 €) – Digital Type: pdf (70 €)
pharmakonpress[at]pharmakonpress[.]gr |
| Έντυπη Μορφή (70 € + έξοδα αποστολής) – Printed Type (70 € + shipping)
pharmakonpress[at]pharmakonpress[.]gr |
|