| Title | Atrial natriuretic peptide suppresses left ventricular function and the renin-angiotensin-aldosterone system activity in normal and moderately atherosclerotic rabbits | |
| Authors | Konstantinos Kallaras¹, George Babas¹, Vassiliki Stergiou-Michailidou¹, Michael Karamouzis² and Thomas Zaraboukas³
Laboratories of 1. Experimental Physiology, 2. Biological Chemistry and 3. Pathology, Faculty of Medicine, Aristotle University, Thessaloniki, Greece |
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| Citation | Kallaras, K., Babas, G., Stergiou-Michailidou, V., Karamouzis, M., Zaraboukas, T.: Atrial natriuretic peptide suppresses left ventricular function and the renin-angiotensin-aldosterone system activity in normal and moderately atherosclerotic rabbits, Epitheorese Klin. Farmakol. Farmakokinet. 20(2): 181-184 (2006) | |
| Publication Date | Accepted for publication: 19-20 May 2006 | |
| Full Text Language | English | |
| Order – Buy | Ηλεκτρονική Μορφή: pdf (10 €) – Digital Type: pdf (10 €)
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| Keywords | ANP, atherosclerosis, left ventricle, angiotensin II, aldosterone. | |
| Other Terms | review article | |
| Summary | Atrial natriuretic peptide (ANP) has, in vivo, negative or no effect on left ventricular (LV) systole and relaxation. The aim of this study was to test the effect of ANP on LV function and on the renin-angiotensin-aldosterone system (RAAS) in normal and moderately atherosclerotic male anesthetized New Zealand White (NZW) rabbits. Each group’s individuals were divided into two subgroups of five animals each. Rabbits were rendered atherosclerotic by receiving for four weeks regular chow supplemented with 2% cholesterol and 6% corn oil. The LV and the left carotid artery were cannulated for left ventricular pressure (LVP) and blood pressure (BP) recordings, with concurrent ECG tracing. The left ventricular maximal pressure (LVPmax), the left ventricular end diastolic pressure (LVEDP), the maximal and the minimal change over time in LVP (LVmaxdp/dt and LVmindp/dt), the Δd (time interval between LVmax dpldt and LVmin dp/dt = systole duration) and the left ventricular ejection time (LVET) were estimated and the Double Product (heart rate X systolic blood pressure – HR x SBP) was calculated. Central venous pressure (CVP) was also recorded from the right jugular vein and blood samples were taken for lipids, ANP, plasma renin activity (PRA) and aldosterone (Ado) levels measurement. Recordings and blood samples for hormones measurement (by RIA) were taken at 0 min) and 20, 40, 60 min following an intravenous 20-min administration of either 0.2 µg/kg/min hANP in 5ml normal saline (NlS) or only 5ml NlS. The results were analyzed by t-test and repeated measures ANOVA. Mild to moderate atherosclerosis was found in rings of ascending aorta. ANP infusion resulted in decrease (p<0.05) of BP in 20min time only, but decrease (p<0.05) of LVPmax from 20min, decrease (p<0.05) of LVEDP and LVmaxdp/dt from 40 min, and less negative change (in- crease) (p<0.05) of LVmindp/dt from 40 min. DP was increased (p<0.05) from 20min only in atherosclerotic animals. PRA and aldo did not differ significantly at 0 min between atherosclerotic and normal; PRA in ANP receivers was smaller (p<0.01) whereas aldo tended to be smaller (p=0.06) than in N/S receivers. It is concluded that ANP administration, in doses to achieve levels approximately three fold the normal range, in normal and moderately atherosclerotic anesthetized NZW rabbits results in a negative effect on LV systolic function and relaxation and suppresses the RAAS activity independently of the atherosclerotic state. | |
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Online ISSN 1011-6575
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