| Title | Effects of pesticides on exposed populations from four European countries | ||
| Authors | S.M. Piperakis1, K. Kontogianni1, G. Karanastasi1, C. Siffel2, A. Cebulska-Wasilewska3, R. Markos4, Z. Iakovidou-Kritsi5 and M.M. Piperakis1
1. Biology Unit, Department of Pre-School Education, University of Thessaly, Volos, Greece 2. Department of Human Genetics and Teratology, B. Johan National Center for Epidemiology, Budapest, Hungary 3. Department of Radiation and Environmental Biology, Niewodniczanski Institute of Nuclear Physics, Krakow, Poland 4. Grup de Mutagenesi, Departament de Genetica i de Microbiologia, Facultat de Ciencies, Universitat Autonoma de Barcelona, 08193 Bellaterra, Spain 5. Laboratory of Biology and Genetics, Medical School, Aristotle University, Thessaloniki, Greece |
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| Citation | Piperakis, S.M., Kontogianni, K., Karanastasi, G., Siffel, C., Cebulska-Wasilewska. A. et al.: Effects of pesticides on exposed populations from four European countries, Epitheorese Klin. Farmakol. Farmakokinet. 22(2): 291-293 (2008) | ||
| Publication Date | 23-25 May 2008 | ||
| Full Text Language | English | ||
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| Keywords | Comet assay, DNA damage, DNA repair, greenhouses, pesticides. | ||
| Other Terms | Review article | ||
| Summary | The potential genetic hazard of pesticides to humans is of great concern, in particular in occupational and environmental settings because of their widespread use for domestic and industrial applications. In the present study the genotoxic potentials of commonly applied pesticides have been evaluated using the single cell gel electrophoresis (comet assay). This method was used to quantify the level of DNA damage in lymphocytes of farmers who were occupationally exposed to pesticides. Particularly, we measured the amount of DNA damage in isolated human peripheral lymphocytes from agricultural workers from Spain, Hungary, Poland and Greece in comparison to healthy men from the same areas who had no previous occupational exposure to pesticides. All results indicated no statistically significant differences in basal DNA damage between our study groups. In addition, exposure of peripheral blood lymphocytes to hydrogen peroxide or γ-irradiation led to a similar degree of additional DNA damage and subsequent repair for all our studied populations. In conclusion the greenhouse workers who participated in this study had no detectable increased DNA damage or alteration in their cellular response to DNA damage from our control populations. | ||
| References | 1. IARC: International Agency for Research on Cancer, Occupational Exposures in Intesticide Application, and Some Pesticides. Vol. 53: IARC Monographs, Lyon, France, 1991
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Online ISSN 1011-6575
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