| Title | Effect of Leguminosae family plant extracts and polyphenolic fractions on topoisomerase I-induced nicking of DNA | ||
| Authors | C. Spanou1, N. Aligiannis2, A.L. Skaltsounis2 and D. Kouretas1
1. Department of Biochemistry & Biotechnology, University of Thessaly, Larissa and 2. Division of Pharmacognosy and Chemistry of Natural Products, School of Pharmacy, University of Athens, Athens, Greece |
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| Citation | Spanou, C., Aligiannis, N., Skaltsounis, A.L., Kouretas, D.: Effect of Leguminosae family plant extracts and polyphenolic fractions on topoisomerase I-induced nicking of DNA, Epitheorese Klin. Farmakol. Farmakokinet. 22(2): 336-338 (2008) | ||
| Publication Date | 23-25 May 2008 | ||
| Full Text Language | English | ||
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| Keywords | Legumes, polyphenols, flavonoids, topoisomerase I. | ||
| Other Terms | Review article | ||
| Summary | Topoisomerase I constitutes one of the enzymes playing crucial role in replication, transcription, recombination, chromosome condensation and maintenance of genome stability. Because it is an essential enzyme for vital functions of DNA during normal cell growth, inhibitors of its activity are considered as promising anticancer agents. Eleven aqueous and two methanolic extracts derived from Leguminosae family plants cultivated in Greece, along with fractions rich in phenolic compounds isolated from the two methanolic extracts of Vicia faba and Lotus edulis were examined for their ability to affect topoisomerase I-induced nicking of DNA, using topoisomerase I relaxation assay. From the tested extracts, only the aqueous extracts of aerial plant parts of Lathyrus laxiflorus subsp. Laxiflorus and Vicia faba and both methanolic extracts of aerial plant parts of Vicia faba and Lotus edulis exhibited the ability to inhibit the nicking activity of topoisomerase I. Among them, the extract derived from Lathyrus laxiflorus subsp. Laxiflorus aerial plant parts was the most potent. As for the flavonoid fractions isolated from the methanolic extracts, the majority of them exhibited significant inhibitory activity which was more potent than that of the initial extracts, implying that the polyphenolic compounds present in the fractions may constitute some of the extract components responsible for the observed properties of the extracts. These results also indicate a mechanism by which these plant extracts and their active principles exert chemopreventive properties and can be considered as possible anticancer agents. | ||
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| Relative Papers | |||
Online ISSN 1011-6575
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Articles published in this Journal are Indexed or Abstracted in: • Chemical Abstracts • Elsevier’s Bibliographic Databases: Scopus, EMBASE, EMBiology, Elsevier BIOBASE SCImago Journal and Country Rank Factor
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