Review of Clinical Pharmacology and Pharmacokinetics – International Edition Volume 38 (2024) – Supplementary Issue 2

Open Access Research

Drug synthesis, separation, and identification of a di-stereoisomeric mixture of spiro-oxalidinonic derivatives of sorbinil by high-performance liquid chromatography

Esaim Fadhel Khalfa^1,*, Rawaa G. Abdullah², Qasim Jawad Al-Daami², Aymen Bash³, Saba Abdulmunem Habeeb Al-Qaysi¹, Ghassan Jasim Mohammed³, Widad Abd Al-Jabbar Mozzan⁴
¹Department of Pharmaceutical Chemistry, College of Pharmacy, University of Babylon, Hillah, Iraq
²Department of Clinical Laboratory Sciences, College of Pharmacy, University of Babylon, Hillah, Iraq
³Department of Pharmaceutics, College of Pharmacy, University of Babylon, Hillah, Iraq
⁴Department of Pharmacology and Toxicology, College of Pharmacy, Al-Mustaqbal University, Hillah, Iraq

*Corresponding author: Esaim Fadhel Khalfa, Department of Pharmaceutical Chemistry, College of Pharmacy, University of Babylon, Hillah, Iraq; Tel.: +964-(0)7809779069
E-mail: phar.esaim.fadhel@uobabylon.edu.iq

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Published: 5 May 2024; https://doi.org/10.61873/ORXR1322

Abstract
A straightforward and sensitive method for high-performance liquid chromatography (HPLC) was created in order to separate and identify the di-stereoisomeric mixture of a spiro-oxalidinonic derivative of sorbinil. HPLC was carried out using a C18 reversed-phase column. The mobile phase used in the isocratic elution process was a 70:30 v/v combination of methanol and acetonitrile flowed at a rate of 2 mL/min. The procedure delivered the best result among the various attempts made. Two peaks were identified on the chromatogram, attributable to the two di-stereoisomers of the mixture analysed. Analogues of sorbinil, a powerful inhibitor of aldose reductase, have been synthesized and further tested on aldose reductase as mixtures of di-stereoisomers, exhibiting an IC₅₀in the order of the micromolar. The structure of the compounds was checked with nuclear magnetic resonance spectroscopy. The chemical shifts were expressed in ppm scale δ.

Keywords: di-stereoisomeric mixture, HPLC, sorbinil, synthesis, drug

Please cite as:
Khalfa E. F., Abdullah R. G., Al-Daami Q. J., Bash A., Al-Qaysi S. A. H., Jasim Mohammed G., Al-Jabbar Mozzan W. A. Drug synthesis, separation, and identification of a di-stereoisomeric mixture of spiro-oxalidinonic derivatives of sorbinil by high-performance liquid chromatography. Rev. Clin. Pharmacol. Pharmacokinet. Int. Ed. 38 (Sup2): 133-136 (2024). https://doi.org/10.61873/ORXR1322