Τόμος 1 (1987) – Τεύχος 2 – Άρθρο 6 – Επιθεώρηση Κλινικής Φαρμακολογίας και Φαρμακοκινητικής-Διεθνής Έκδοση – Volume 1 (1987) – Issue 2 – Article 6 – Epitheorese Klinikes Farmakologias και Farmakokinetikes-International Edition

Title

La spectrometrie de masse: une excellente methode quantitative tres utile en pharmacologie
Authors Franz J. Buchholzer, Maria Koufaki et Jean M.J. Tronchet

Laboratoire CPOP, Sciences II, Universite de Geneve

Citation Buchholzer, F.J., Koufaki, M. and Tronchet, J.M.J.: La spectrometrie de masse: une excellente methode quantitative tres utile en pharmacologie, Epitheorese Klin. Farmakol. Farmakokinet. 1(2): 119-130 (1987)
Publication Date 1987-10
Full Text Language French
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Keywords Mass spectrometry, pharmacology, gas chromatrograph, LC-MS, GC-MS, ions, monitoring, quantitative.
Other Terms review article
Summary Although mass spectrometry is a relatively old chemical and instrumental method, its use in pharmacology began with the development of the combined GC-MS in 1960. This powerful analytical system combines the mass spectrometer’s unique identification potential and the gas chromatrograph’s separation capabilities. The first quantitative application of this technique has been reported in 1970 when the combined instrument was used to determine blood concentrations of drugs and drug metabolites. The combination LC-MS appears promising but will require significant improvements before being widely accepted in pharmacology research. The method which is most used to quantify compounds by GC-MS or LC-MS, is to monitor the intensity of a limited number of ions, characteristic of the compounds being determined, by switching the mass spectrometer analyser rapidly between these ions. This method is called “selected ion monitoring” (SIM) and will be developed here. In this mode the mass spectrometer is used as a mass filter and respond selectively to ions of predetermined mass. Quantitative mass spectrometry requires two fundamental processes for successful analysis: a) the m.s. response must be calibrated with standard quantities of analyte, b) a suitable internal standard should be chosen for optimal sensitivity and specificity. Several papers cover applications of this technique and some examples will be mentioned here.
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