| Title | Effects of proteolytic enzymes papain, chymotrypsin and trypsin on the guinea pig skin, with special reference to the hair follicle | |
| Authors | Evangelia E. Protopapa¹, Alexandra Tsigonia-Primikiridou¹ and Nicolaos G. Stavrianeas²
1. Technological Educational Institution of Athens, Egaleo, Athens, Greece 2. Dermatological Clinic of the Medical School of the University of Athens, Athens, Greece |
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| Citation | Protopapa, E.E., Tsigonia-Primikiridou, A., Stavrianeas, N.G.: Effects of proteolytic enzymes papain, chymotrypsin and trypsin on the guinea pig skin, with special reference to the hair follicle, Epitheorese Klin. Farmakol. Farmakokinet. 8(2): 84-88 (1994) | |
| Publication Date | Received for publication: 30 June 1994
Accepted for publication: 25 July 1994 |
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| Full Text Language | English | |
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| Keywords | Enzymes, papain, chymotrypsin, trypsin, skin, hair follicle, guinea pig, epilation, histology. | |
| Other Terms | review article | |
| Summary | In studies concerning the depilatory action of proteolytic enzymes, the effect of three such enzymes, chymotrypsin, papain and trypsin, applied by iontophoresis on guinea pig skin, was studied at the histological level. All enzymes, applied in concentrations of 0.1-1 mg/ml, produced moderate to very intense effects, such as the presence of epithelial cells in the lumen of cystic dilated hair follicles, detachment of inner root sheath and dystrophic follicles. The action of chymotrypsin, followed immediately by papain, produced strong degenerative acantholytic changes. The effects appeared after one minute iontophoresis of the enzymes and a further thirty minutes of interaction period and were maximal if the period of enzyme iontophoresis was increased to five minutes. | |
| References | 1. Yates, J.R.: Studies in depilation. VII: Structural changes in the wool follicle during depilation with acid an enzyme systems. Austr. J. Biol. Sci. 21: 1249-1261 (1968)
2. Bouclier, M.r Hensby, C.N., Cavey, D.: Animal models used in cutaneous pharmacology. In: C.L. Galli, C.N. Hensby, M. Mrinovich, eds, Recent Advances, NATO ASI Series A, Life Sciences, 181: 99- 120 (1990) 3. Ogura, R., Knox, J.M., Griffin, A.C.: Separation of epidermis for the study of epidermal sulphydryl. J. Invest Derm. 35: 239-243 (1960) 4. Medawar, P.B.: Sheets of pure epidermal epithelium from human skin. Nature 148. 783 (1941) 5. Fan, J.: Epidermal separation with purified X trypsin. J. invest. Derm. 30: 271-272 (1958) |
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| Relative Papers |
Online ISSN 1011-6575
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Articles published in this Journal are Indexed or Abstracted in: • Chemical Abstracts • Elsevier’s Bibliographic Databases: Scopus, EMBASE, EMBiology, Elsevier BIOBASE SCImago Journal and Country Rank Factor
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