| Title | B-cell epitopes of the La/SSB autoantigen: anti-La/SSB specificity in patients with autoimmune rheumatic diseases | |
| Authors | Efthalia Yiannaki1, Athanasios Tzioufas2, Maria Sakarellos-Daitsiotis3, Constantinos Sakarellos3 and Charalampos Moutsopoulos2
1. Department of Pathology, Medical School, University of Ioannina, 451 10 Ioannina, Greece 2. Department of Pathophysiology, Medical School, University of Athens, M. Asias 75, 11527 Athens, Greece 3. Department of Chemistry, Section of Organic Chemistry and Biochemistry, University of Ioannina, 45110 Ioannina, Greece |
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| Citation | Yiannaki, E., Tzioufas, A., Sakarellos-Daitsiotis, M., Sakarellos, C., Moutsopoulos, C.: B-cell epitopes of the La/SSB autoantigen: anti-La/SSB specificity in patients with autoimmune rheumatic diseases, Epitheorese Klin. Farmakol. Farmakokinet. 11(2-3): 94-96 (1997) | |
| Publication Date | 1997 | |
| Full Text Language | English | |
| Order – Buy | pharmakonpress[at]pharmakonpress[.]gr | |
| Keywords | La/SSB autoantigen, B-cell epitopes. | |
| Other Terms | review article | |
| Summary | La/SSB autoantigen is a ribonucleoprotein particle consisting of a protein component against which are often directed autoantibodies in sera of patients with primary Sjogren’s Syndrome (pSS) and Systemic Lupus Erythematosus (SLE). The purpose of the present study is to investigate the fine specificity of these autoantibodies by determining the B-cell epitopes of the La/SSB autoantigen. The epitope mapping of the La/SSB protein was performed using overlapping 20mer synthetic peptides covering the entire sequence (408 amino acids) of the protein. Purified IgG from sera of SLE and pSS patients were tested against these overlapping synthetic peptides. Peptide sequences spanning the regions 145-164, 289-308, 301-320 and 349-368 of the La/SSB protein were found highly reactive with the purified IgG. Biotinylated synthetic soluble peptides corresponding to the minimum required length of the epitopes were also prepared. The defined epitopes:
147HKAFKGSI154, 291NGNLQLRNKEVT302, 301VTWEVLEGEVEKEALKKI318, 349GSGKGKVQGKKTKF364 showed significant sensitivity and specificity for the anti-La/SSB antibodies of about 94 and 86% respectively. |
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2. Chan E.K.L., Tan E.M.: Mot. Cell Biol. 7: 2588 (1987) 3. Sturgess A.D., Peterson M.G., McNeiiage L.I., Whitting-ham S., Coppel R.L.: J. Immunol. 140: 3212 (1988) 4. St Clair E.W., Pisetsky D.S., Reich C.F., Keene J.D.: J. Immunol. 141: 4173 (1988) 5. Geysen H.M., Rodda S.J., Mason T.J., Schoofs P.G.: J. Immunol. Methods 202: 259 (1987) 6. Stewart J.M., Young J.D. (eds): Solid phase peptide synthesis. Pierce Chemical Company, Rockford, IL.. USA, 1984 |
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Online ISSN 1011-6575
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