| Title | In vitro model for the evaluation of preservatives phototoxic activity | |
| Authors | Vassiliki Kefala, Ioanna Kostara, Haris Karageorgiou and Dionysios D. Varonos
Department of Experimental Pharmacology Medical School, University of Athens, 75, Mikras Asias St., Goudi, Athens, Greece |
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| Citation | Kefala, V., Kostara, I., Karageorgiou, H., Varonos, D.D.: In vitro model for the evaluation of preservatives phototoxic activity, Epitheorese Klin. Farmakol. Farmakokinet. 14(2): 93-96 (2000) | |
| Publication Date | Received for publication: 25 October 1999
Accepted for publication: 30 November 1999 |
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| Full Text Language | English | |
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| Keywords | Preservatives, phototoxicity, photoirritation, Saccharomyces cerevisiae. | |
| Other Terms | review article | |
| Summary | The phototoxicity and the photoirritation have been defined in the past through various methods of experimentation on animals and volunteers in order for some substances to be safely used by humans. The methods requiring the use of animals are disadvantageous, because, on the one hand, they demand the killing of lots of animals and on the other they have a high cost and the application may be dangerous for the volunteers. In our method, we use ultraviolet radiation (UV-A) in order to radiate the substances we are studying during their diffusion from a paper disc placed upon agar freshly seeded with Saccharomyces cerevisiae (Fleisch-man’s yeast) (1). As positive control we use 8- Metgoxypsoralen (8-MOP) at a concentration of 10-2 %. The creation of dear zones of inhibition of Saccharomyces cerevisiae yeast development suggests the phototoxic effect of the substance we are studying. Our method is inexpensive, simple and gives results within a short period of time, which is in 61 hours. The correlation with positive human phototoxicity results is excellent, so that the protection of the consumers is successful. | |
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| Relative Papers |
Online ISSN 1011-6575
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Articles published in this Journal are Indexed or Abstracted in: • Chemical Abstracts • Elsevier’s Bibliographic Databases: Scopus, EMBASE, EMBiology, Elsevier BIOBASE SCImago Journal and Country Rank Factor
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