Title | OS-9 is a potent anti-apoptotic factor that also promotes cell viability | |
Authors | E. Vourvouhaki¹, S.M. Sullivan¹, Y. de Nantois¹, S.K. Smith¹,², C.G. Print² and D.S. Charnock-Jones¹,²
Reproductive Molecular Research Group. 1. Department of Obstetrics and Gynaecology, University of Cambridge, Cambridge, CB2 2SW, United Kingdom 2. Department of Pathology, University of Cambridge, Cambridge, CB2 1QP, United Kingdom |
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Citation | Vourvouhaki, E., Sullivan, S.M., de Nantois, Y., Smith, S.K., Print, C.G. et al.: OS-9 is a potent anti-apoptotic factor that also promotes cell viability, Epitheorese Klin. Farmakol. Farmakokinet. 21(3): 235-250 (2007) | |
Publication Date | Accepted for publication (Final version): September 10, 2007 | |
Full Text Language | English | |
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Keywords | Cell survival, programmed cell death, cell fate. | |
Other Terms | review article | |
Summary | An Affymetrix array analysis of serum starved endothelial cells (1) identified a number of known transcripts and ESTs of unknown function that appeared to be regulated as apoptosis was initiated. Here we describe the analysis of one of these ESTs, designated 36996 at on the Affymetrix chips, which is identical with the known gene osteosarcoma-9 (OS-9 GI: 580310). The cDNA for OS-9 was cloned and overexpressed in the murine FDC-P1 cell line in the presence and absence of IL3, staurosporine and TNF-a. Overexpression of OS-9 had no effect on the incidence of apoptosis in cells deprived of IL3, but conferred resistance to apoptosis induced by staurosporine or TNF-a. Furthermore, treating HUVECs with siRNA directed against OS-9 caused cells to enter the apoptotic program earlier than cells in which OS-9 functions normally. In addition, OS-9 promoted cell viability in both cell types under all conditions. We conclude that OS-9 has a role in cell fate, specifically in encouraging cell viability and conferring resistance to apoptosis. | |
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