| Title | Effect of recombinant platelet-activating factor acetylhydrolase on acetaminophen-induced experimental cell proliferation | |
| Authors | A.D. Grypioti, PhD¹,²,3, M. Mykoniatis, PhD¹, C.A. Demopoulos, PhD² and G. Kostopanagiotou, PhD¹
1. Department of Experimental Pharmacology, Medical School, National and Kapodistrian University of Athens, Greece 2. Department of Chemistry, National and Kapodistrian University of Athens, Greece 3. BioAnalytica, Biotechnology and Analytical Systems |
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| Citation | Grypioti, A.D., Mykoniatis, M., Demopoulos, C.A., Kostopanagiotou, G.: Effect of recombinant platelet-activating factor acetylhydrolase on acetaminophen-induced experimental cell proliferation, Epitheorese Klin. Farmakol. Farmakokinet. 20(2): 165-167 (2006) | |
| Publication Date | Accepted for publication: 19-20 May 2006 | |
| Full Text Language | English | |
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| Keywords | Paracetamol (acetaminophen), PAF, rPAF-AH, liver, injury, regeneration. | |
| Other Terms | review article | |
| Summary | Aim of this study was to investigate the effects of PAF inactivator, recombinant PAF-acetylhydrolase (rPAF-AH) on post acetaminophen-treatment functional outcome of the liver in the rat. Fifty male Wistar rats were divided into two groups: the control group received a toxic dose of acetaminophen (3.5 g/Kg BW) and the rPAF-AH-treated group received the same dose of acetaminophen followed by a dose of rPAF-AH (10 mg/Kg BW) intraperitoneally. The animals were sacrificed at time points of 56, 66, 72, 84 and 96 after acetaminophen treatment. The hepatic injury was evaluated by determination of AST, ALT and ALP activities. Liver regeneration was estimated by [3H]thymidine incorporation into hepatic DNA, liver thymidine kinase activity and hepatocyte mitotic index. The positive effects of rPAF-AH were expressed by high decrease of hepatic injury and diminution of regenerating activity. These results indicate that the use of PAF inactivator enhances liver’s recovery from acetaminophen intoxication and attenuates the severity of experimental liver injury providing important means of improving liver function following acetaminophen intoxication. | |
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Online ISSN 1011-6575
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