Title | Caspase-3 activity in pregnant rat spleen and lymph nodes after treatment with cycloheximide and the development of malignant breast tumor in experimental animals: case report | ||
Authors | H. Frangou1, E. Nikoloussis2, N. Massouridou1, T. Vavilis1 and E.-N. Emmanouil2
1. Deparment of General Biology and 2. Laboratory of Histology – Embryology & Anthropology, Medical School, Aristotle University of Thessaloniki, Greece |
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Citation | Frangou, H., Nikoloussis, E., Massouridou, N., Vavilis, T., Emmanouil, E.-N.: Caspase-3 activity in pregnant rat spleen and lymph nodes after treatment with cycloheximide and the development of malignant breast tumor in experimental animals: case report, Epitheorese Klin. Farmakol. Farmakokinet. 22(2): 141-144 (2008) | ||
Publication Date | 23-25 May 2008 | ||
Full Text Language | English | ||
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Keywords | Cycloheximide, caspase-3, spleen, lymph nodes, malignant breast tumor. | ||
Other Terms | Review article | ||
Summary | Cycloheximide (CHX) was found to induce teratogenesis and apoptosis in some cell types, tissues and organs. Although toxic, CHX is used as a pesticide/infecticide drug for plants and fruits. During an extended research protocole we have identified the development of a malignant breast tumor in a rat treated with CHX. Therefore we have tried to investigate the mechanism of action of CHX determining caspase-3 activity in subcellular fractions of spleen and lymph nodes related to the tumor of the rat which presented this tumor during and after gestational period. Caspase-3 activity was determined in crude, nuclear, and cytosolic plus ribosomal fraction of spleen and lymph nodes by a colorimetric assay (kit, Sigma). Our results indicate enhanced caspase-3 activity in subcellular fractions of the above tissues. From our findings we support the theory that CHX may induce apoptosis by enhancing caspase-3 activity in pregnant rat spleen and lymph nodes during malignant breast tumor development. | ||
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Online ISSN 1011-6575
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