| Title | In Vitro responses of the guinea pig gastrointestinal tract to homologous motilin | ||
| Authors | M. Koutsoviti-Papadopoulou1, L. Xu2, I. Depoortere2, L. Thielemans2, Th. Thijs2 and Th.L. Peeters2
1. Laboratory of Pharmacology, Veterinary Faculty, Aristotle University, Thessaloniki, Greece 2. Gut Peptide Laboratory, Faculty of Medicine, University of Leuven, Belgium |
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| Citation | Koutsoviti-Papadopoulou, M., Xu, L., Depoortere, I., Thielemans, L., Thijs, T. et al.: In Vitro responses of the guinea pig gastrointestinal tract to homologous motilin, Epitheorese Klin. Farmakol. Farmakokinet. 22(2): 207-211 (2008) | ||
| Publication Date | 23-25 May 2008 | ||
| Full Text Language | English | ||
| Order – Buy | Ηλεκτρονική Μορφή: pdf (10 €) – Digital Type: pdf (10 €)
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| Keywords | Guinea pig motilin, porcine motilin, intestine, contractility, electrical field stimulation. | ||
| Other Terms | Review article | ||
| Summary | Following the isolation of the guinea pig motilin (gp mo), we studied its potency, in comparison with the porcine one, in the rabbit duodenum, and its bio-activity in both unstimulated and stimulated guinea pig antrum and intestinal strips. In the rabbit duodenum the pED50 values for gp and porcine motilin were 8.35 and 8.79, respectively. In the guinea pig, the responses to gp mo were rather weak, since a high concentration was needed to obtain them. 70% of the unstimulated segments responded to 10-6 M gp mo, with jejunum and ileum to be the most sensitive and to give a mean response of 10% of the response to 10-4 M Ach, reaching in the ileum a maximum of 30%; the mean responses of the duodenum and the ascending colon were less than 5%, reaching a maximum close to 10%. This effect was antagonized by atropine and tetrodotoxin (TTX), and, as far as the small intestine is concerned, by the 5HT4-antagonist GR-113808. The reproducibility of the data was very small in the stimulated strips, since only 30% of them responded to gp mo. A shift in the base line in the longitudinal strips and a potentiation of responses to electrical field stimulation (EFS) in the circular strips of the duodenum and ileum were observed. In conclusion, guinea pig and porcine motilin exhibit comparable potencies in the rabbit. In the guinea pig, the weak responses obtained for gp mo seem to be both muscle- and neurally-mediated. | ||
| References | 1. Segawa T., Nakano M., Kai Y., Kawatani H., Yajima H.: Effect of synthetic motilin and related polypeptides on contraction of gastrointestinal smooth muscle. J. Pharm. Pharmacol. 28: 650-651(1976)
2. Minocha A., Galligan J.J.: Erythromycin inhibits contractions of nerve-muscle preparations of the guinea pig small intestine. J. Pharmacol.Exp. Ther. 257: 1248-1252 (1991) 3. Peeters T.L., Macielag M.J., Depoortere I., Konteatis Z.D.: Florance JR, Lessor RA, Galdes A. D-amino acid and alanin scans of the bioactive portion of porcine motilin. Peptides 13: 1103-1107 (1992) 4. Okwuasaba, 1978 (Ph.D Thesis). Mechanisms of Motilin Ecxitation, by Fox J.E.T., In: (Itoh Z, ed.) Motilin. Pp. 73-91, Academic Press, 1990 5. Furness J.B., Clark M.J., Wright T., Bertrand P.P., Bornsyein J.C., Verlinden M.: An action of erythromycin in the intestine that is not mediated via motilin receptors. Clin. Exp. Pharmacol. Physiol. 26: 100-1004 (1999) 6. Xu L, Depoortere I, Tang M, Peeters TL. Identification and expression of the motilin precursor in the guinea pig. FEBS Lett. 490: 7-10 (2001) 7. Harada N., Chijiiwa Y., Misawa T., Yoshinaga M., Nawata H.: Direct contractile effect of motilin on isolated smooth muscle cells of guinea pig small intestine. Life Sci. 51: 1381-1387 (1992) 8. Tack J. Georges Brohee Prize 1994: Motilin and the enteric nervous system in the control of interdigestive and postprandial gastric motility. Acta Gastroenterol. Belg. 58: 21-30 (1995) 9. Poitras P., Lahaie R.G., St-Pierre S., Trudel L.: Comparative stimulation of motilin duodenal receptor by porcine or canine motilin. Gastroenterology 92: 658-662 (1987) |
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Online ISSN 1011-6575
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Articles published in this Journal are Indexed or Abstracted in: • Chemical Abstracts • Elsevier’s Bibliographic Databases: Scopus, EMBASE, EMBiology, Elsevier BIOBASE SCImago Journal and Country Rank Factor
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