| Title | TGF-b expression in the neonatal rat spinal cord after sciatic nerve injury | |
| Authors | D. Kapoukranidou, B. Michailidou, A. Kritis and M. Albani
Department of Physiology and Pharmakology, Medical School, Aristotle University, Thessaloniki, Greece |
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| Citation | Kapoukranidou, D., Michailidou, B., Kritis, A., Albani, M.: TGF-b expression in the neonatal rat spinal cord after sciatic nerve injury, Epitheorese Klin. Farmakol. Farmakokinet. 20(2): 192-194 (2006) | |
| Publication Date | Accepted for publication: 19-20 May 2006 | |
| Full Text Language | English | |
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| Keywords | TGF-b, axotomy, motoneuron, immunochemistry. | |
| Other Terms | review article | |
| Summary | Sciatic nerve injury in the neonatal rat leads to motoneuron degeneration through mechanisms that are not fully understood yet. Various factors are implicated in this procedure among which, a multifunctional cytokine, the TGF-b. In this essay, we studied the possible changes in TGF-b expression in the neonatal rat spinal cord after unilateral sciatic nerve injury. A sciatic nerve injury was caused by a forceps on 7 days old wistar rats. The rats were sacrificed and their spinal cords were removed on 12, 24, 48, 72 hours, 1 week and 1 month, following nerve injury. Cryostat sections 0,4µ were taken and immunostaining was carried out using an anti-TGF-b1 antibody. TGF-b immunostaining was observed within spinal cord motoneurons of the 12 hour group and the 1 month group after axotomy, while in the rest of the groups there was no difference between controls and operated animals. No TGF-b immunostaining was observed in any of the controls. Our findings agree with the existing literature that TGF-b expression is increased as a result to injury, and thus support the theory of its neuroprotective action. Nevertheless, the relevant mechanisms of action still remain unclear. | |
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Online ISSN 1011-6575
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