| Title | Signal transduction differences between WT and polymorphic α2Β-adrenergic receptor in LLC-PK1 Cells | |
| Authors | Orthodoxia Mastrogianni¹, George Karkoulias¹, Herve Paris² and Christodoulos Flordellis¹
1. Department of Pharmacology, School of Medicine, University of Patras, 26110 Rio Patras, Greece 2. INSERM U388, Institut Louis Bugnard, CHU Rangueil, Toulouse, France |
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| Citation | Mastrogianni, Ο., Karkoulias, Γ., Paris, Η., Flordellis, Ch: Signal transduction differences between WT and polymorphic α2Β-adrenergic receptor in LLC-PK1 Cells, Epitheorese Klin. Farmakol. Farmakokinet. 20(2): 249-250 (2006) | |
| Publication Date | Accepted for publication: 19-20 May 2006 | |
| Full Text Language | English | |
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| Keywords | α2Β-adrenergic receptor, MAPK, Akt, NF-κB, IKKα/β. | |
| Other Terms | review article | |
| Summary | The aim of the present study was to characterise the signaling pathway triggered by activated human α2B-adrenergic receptor in trasfected LLC-PK1, a renal tubular cell line. Moreover to investigate differences between the wild-type (WT) and a polymorphic α2B-AR (MUT), which has a deletion of three glutamic acids (Del301-303) in its third intracellular loop. Upon agonist stimulation with UK 14304, both receptors (WT and MUT) induced ERK1/2 phosphorylation, whilst this phosphorylation was abolished by prior treatment of the cells with LY 240092, a pharmacological inhibitor of PI3K. Treatment of the cells with UK 14304 induced IKK phosphorylation and NF-KB-driven luciferase activity and this activation was abolished by prior treatment of the cells with LY 240092. The activation of IKK/NF-κB from the polymorphic adrenoceptor was absolutely dependent on prior ERK activation, in clear demarcation to WT-induced NF-κB activation, which is ERK independent. | |
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Online ISSN 1011-6575
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| ΕΤΗΣΙΑ ΣΥΝΔΡΟΜΗ 2006 – ANNUAL SUBSCRIPTION 2006 | |
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