| Title | Corticosteroids and ß2-adrenergic agonists differentially modulate the synthesis and secretion of glycosaminoglycans by human lung cells | |
| Author | E. Papakonstantinou1, M. Roth2, M. Tamm2 and G. Karakiulakis1
1Dept Pharmacology, School of Medicine, Aristotle University, 54124 Thessaloniki, Greece and 2Pulmonary Cell Research, Univ. Hospital Basel, Switzerland, 4031 Basel, Switzerland |
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| Citation | Papakonstantinou, E., Roth, M., Tamm, M. and Karakiulakis, G.: Corticosteroids and ß2-adrenergic agonists differentially modulate the synthesis and secretion of glycosaminoglycans by human lung cells, Epitheorese Klin. Farmakol. Farmakokinet. 18(1): 156-160 (2004) | |
| Publication Date | Accepted for publication: 2004 | |
| Full Text Language | English | |
| Order – Buy | Ηλεκτρονική Μορφή: pdf (10 €) – Digital Type: pdf (10 €) pharmakonpress[at]pharmakonpress[.]gr | |
| Keywords | β2-Adrenergic Agonists, Glycosaminoglycans, GAGs | |
| Other Terms | review article | |
| Summary | Asthma is characterized by airway remodelling, involving changes in deposition of extracellular matrix molecules. First-line therapy of persistent asthma involves the combination of inhaled corticosteroids and 02 adrenergic agonists. The aim of this study was to investigate the effect of corticosteroids, 02 agonists and their combination in the secretion and deposition of glycosaminoglycans (GAGs) by human lung cells. Human bronchial epithelial and smooth muscle cells and lung fibroblasts were incubated for 48 h in the presence of budesonide, ciclesonide, formoterol or salmeterol. GAGs were determined in the cell culture medium and in the cell-associated matrix by 3H-glucosamine incorporation. We found that budesonide and ciclesonide resulted in a dose-dependent decrease in both cell-associated and secreted GAGs to approximately 50% of control levels. This effect was inhibited by the corticosteroid antagonist mifepristone, indicating the involvement of corticosteroid receptors. Formoterol and salmeterol had no effect. However, the combination of β2 agonists with corticosteroids further enhanced the inhibitory effect of corticosteroids. This effect was mediated via adrenergic receptors since it was abolished by propranolol. These results demonstrate that the anti-inflammatory action of corticosteroids when used alone or in combination with 02 adrenergic agonists in the treatment of asthma may also be associated with a beneficiary decrease in the deposition of matrix molecules in the lung. | |
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Online ISSN 1011-6575
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Articles published in this Journal are Indexed or Abstracted in: • Chemical Abstracts • Elsevier’s Bibliographic Databases: Scopus, EMBASE, EMBiology, Elsevier BIOBASE SCImago Journal and Country Rank Factor
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