Τόμος 2 (1984) – Τεύχος 3 – Άρθρο 7 – Επιθεώρηση Κλινικής Φαρμακολογίας και Φαρμακοκινητικής-Ελληνική Έκδοση – Volume 2 (1984) – Issue 3 – Article 7 – Epitheorese Klinikes Farmakologias και Farmakokinetikes-Greek Edition

High-Pressure Liquid Chromatographic Determination of Verapamil and Nor-Verapamil in Serum

JULIA ATTA-POLITOU

^{Εργαστήριο Τοξικολογίας, Πανεπιστημιακό Νοσοκομείο Leiden (Ολλανδία)}

2: 247-254 (1984)

PHARMAKON-Press: Epitheorese Klin. Farmakol. Farmakokinet.

2: 247-254 (1984)

verapamil, nor-verapamil

A simple high-performance liquid chromatographic method for the simulta­neous measurement of serum verapamil and nor-verapamil has been developed. The sample (200 μl) is vortex-mixed for 30 sec with 4Ν sodium hydroxide solution (100 μl), internal standard solution (aqueous D-517 1 μg/ml) (100 μl) and methyl-tert-butyl ether (400 μl). After centrifugation at 3000 xg for 30 sec a portion (100 μl) of the resulting extract is ana­lyzed on a (5 μ) silica column (125 x 5 mm I.D.) using methanolic solution of ΝΗ₄CIO₄ (10 mM) as the mobile phase. The column effluent is monitored by fluorescence detection using an excitation wavelength of 203 nm. The limit of accurate measurement of the assay is 1,5 μg/L and no potential sources of interference have been identified. The method has advantages of speed, small sample requirement and satisfa­ctory resolution of the three major metaboli­tes of verapamil.

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