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Τίτλος – Title
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Η Επίδραση της Φαιντανύλης στην Απελευθέρωση Γλουταμικού από τον πρόσθιο Υποθάλαμο Επίμυων Effects of Fentanyl Administration on the Release of Glutamate in the Rat Posterior Hypothalamus |
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Συγγραφέας – Author
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Χ. Πουρζιτάκη1, Χ. Σαρδέλη1, Γ. Παπαζήσης1, Μ. Χουρδάκης 1,2, Α. Αμανίτη2, Δ. Κούβελας1 12ο Εργαστήριο Φαρμακολογίας, 2 Κλινική Αναισθησιολογίας και Εντατικής Θεραπείας, Ιατρική Σχολή, Αριστοτέλειο Πανεπιστήμιο Θεσσαλονίκης C. Pourzitaki1, C. Sardeli1, G. Papazisis1, M. Hourdakis1,2, A. Amaniti2, D. Kouvelas1 1Laboratory of Pharmacology, 2Clinic of Anesthesiology and Intensive Care, School of Medicine, Aristotle University, Thessaloniki, Hellas |
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Παραπομπή – Citation
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Πουρζιτάκη,Χ., Σαρδέλη,Χ., Παπαζήσης,Γ., Χουρδάκης,Μ., Αμανίτη,Α., Κούβελας,Δ. : Η Επίδραση της Φαιντανύλης στην Απελευθέρωση Γλουταμικού από τον πρόσθιο Υποθάλαμο Επίμυων, Επιθεώρηση Κλιν. Φαρμακολ. Φαρμακοκινητ. 27: 40-41 (2009)
Pourzitaki,C., Sardeli,C., Papazisis,G., Hourdakis,M., Amaniti,A., Kouvelas,D. : Effects of Fentanyl Administration on the Release of Glutamate in the Rat Posterior Hypothalamus, Epitheorese Klin. Farmakol. Farmakokinet. 27: 40-41 (2009)
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Ημερομηνία Δημοσιευσης – Publication Date
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8 Μαρτίου 2009 – 2009-03-08
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Γλώσσα Πλήρους Κειμένου –
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Ελληνικά – Greek |
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Λέξεις κλειδιά – Keywords
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Fentanyl, hypothalamus, rat, glutamate, push-pull superfusion, HPLC
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Λοιποί Όροι – Other Terms
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Κλινική Μελέτη Clinical Trial |
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Περίληψη – Summary
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– Fentanyl, an m-opioid receptor agonist, has long been studied for its neuropharmacological effects. However, its action on the release of neurotransmitters is not totally understood. The results from studies on the cardiovascular changes after fentanyl administration are conflicting. In the present study the action of fentanyl on the concentration of the excitatory and inhibitory aminoacids is investigated. We examined the effects of continuous intravenous (10 μg/kg) as well as intrahypothalamic (50 nmol/min) fentanyl administration on the release rates of glutamate in the superfusate of hypothalamus, under tail pinch manipulation. The release of the neurotransmitter was monitored by the push–pull superfusion technique. To investigate the role of fentayl we also administered an opioid antagonist, naloxone 0.01 mg/kg iv, or 0.1 nmol/min intrahypothalamicaly. Glutamate was determined in the above solution by High Performance Liquid Chromatography (HPLC) and fluorimetric detection after OPA derivatisation. After intravenous fentanyl administration a significant decrease of glutamate was observed. However during the pain manipulations the release rates of glutamate was increased. Intravenous and intrahypothalamic fentanyl administration caused fluctuations in blood pressure and heart rate. Naloxone iv failed to reverse the cardiovascular as well as neurological effects, while intrahypothalamic antagonist administration reversed the alterations neurotransmitter release rate. We conclude that fentanyl effects are related with glutaminergic system activation. There is a possibility that the changes of the amino acid concentration in hypothalamus are related with the parasympathetic inhibition after continuous fentanyl administration. |
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Αναφορές – References
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1. Desole M.S., Esposito G., Fresu L., Migheli R., Enrico P., Mura M.A., De Natale G., Miele E., Miele M.: Effects of morphine treatment and withdrawal on striatal and limbic monoaminergic activity and ascorbic acid oxidation in the rat, Brain Res. 723: 154-161 (1996)
2. Hakan R.L., Henriksen S.J.: Opioid influences on nucleus accumbens neuronal electrophysiology: dopamine and non-dopamine mechanisms. J. Neurosci. 9: 3538-3546 (1989) 3. Hao Y., Yang J.Y., Guo M., Wu C.F.: Morphine decreases extracellular levels of glutamate in the anterior cingulate cortex: an in vivo microdialysis study in freely moving rats. Brain Res. 1040: 191-196 (2005) 4. Holtman J.R., Sloan J.W., Wala E.P.: Morphine tolerance in male and female rats. Pharmacol. Biochem. Behav. 77: 517-523 (2004) 5. Serra P.A., Susini G., Rocchitta G., Migheli R., Dessanti G., Miele E., Desole M.S., Miele M.: Effects of sufentanil on the release and metabolism of dopamine and scorbic acid and glutamate release in the striatum of freely moving rats. Neurosci. Lett. 344: 9-12 (2003) 6. Singewald N.K.D., Chen F., Philippu A.: The release of inhibitory amino acids in the hypothalamus is tonically modified by impulses from aortic baroreceptors as a consequence of blood pressure fluctuations. Naunyn Schmiedebergs Arch. Pharmacol. 356: 348-355 (1997) 7. Singewald N., Chen F., Guo L.J., Philippu A.: Ionic and haemodynamic changes influence the release of the excitatory amino acid glutamate in the posterior hypothalamus. Naunyn Schmiedebergs Arch. Pharmacol. 352: 620-625 (1995) 8. Yoshida S.K., Hirose N., Takada K., Tomiyama K., Koshikawa N.: Cools aAR: Fentanyl increases dopamine release in rat nucleus accumbens: Involvement of mesolimbic mu- and delta-2-opioid receptors. Neuroscience 92: 1357-1365 (1999) 9. Yan-Gang Sun X-LG, Long-Chuan Yu: The neural pathway of galanin in the hypothalamic arcuate nucleus of rats: Activation of beta-endorphinergic neurons projecting to periaqueductal gray matter. J. Neurosci. Res. 85: 2400-2406 (2007) 10. Paxinos G.W.H.: The Rat Brain in Stereotaxic Coordinates. Fourth edition, Academic Press, Sidney, 1998 |
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Online ISSN 1011-6575
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