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Τίτλος – Title
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Σχέση του Πολυμορφισμού Glu298Asp του Γονιδίου της Ενδοθηλιακής Συνθάσης του Μονοξειδίου του Αζώτου e-NOS με το Έμφραγμα του Μυοκαρδίου σε Έλληνες Ασθενείς Relationship of Missence Glu298Asp Variant of the Endothelial Nitric Oxide Synthase Gene and Myocardial Infarction in the Greek Population |
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Συγγραφέας – Author
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Χάιδω Δαφνή1,2, Νικόλαος Δρακούλης1,2, Διονύσιος Κόκκινος3 1Τομέας Φαρμακευτικής Τεχνολογίας, Φαρμακευτικό Τμήμα, Πανεπιστήμιο Αθηνών, 2Eργαστήριο Φαρμακολογίας, Research Diagnostics, Τεχνοβλαστός Πανεπιστημίου Αθηνών, 3Ωνάσειο Καρδιοχειργουργικό Κέντρο, Αθήνα Chaido Dafni1,2, Nikolaos Drakoulis1,2, Dennis V. Cokkinos3 1Department of Pharmaceutical Technology, School of Pharmacy, University of Athens, 2Research Diagnostics, Spin-off Company of the University of Athens, 31st Cardiology Department, Onassis Cardiac Surgery Center, Athens, Hellas |
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Παραπομπή – Citation
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Δαφνή,Χ., Δρακούλης,Ν., Κόκκινος,Δ. : Σχέση του Πολυμορφισμού Glu298Asp του Γονιδίου της Ενδοθηλιακής Συνθάσης του Μονοξειδίου του Αζώτου e-NOS με το Έμφραγμα του Μυοκαρδίου σε Έλληνες Ασθενείς, Επιθεώρηση Κλιν. Φαρμακολ. Φαρμακοκινητ. 27: 23-26 (2009)
Dafni,Ch., Drakoulis,N., Cokkinos,D. : Relationship of Missence Glu298Asp Variant of the Endothelial Nitric Oxide Synthase Gene and Myocardial Infarction in the Greek Population, Epitheorese Klin. Farmakol. Farmakokinet. 27: 23-26 (2009)
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Ημερομηνία Δημοσιευσης – Publication Date
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8 Μαρτίου 2009 – 2009-03-08
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Γλώσσα Πλήρους Κειμένου –
Full Text Language |
Ελληνικά – Greek |
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Παραγγελία – Αγορά –
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Ηλεκτρονική Μορφή: pdf (15 €) –
Digital Type: pdf (15 €) pharmakonpress[at]pharmakonpress[.]gr |
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Λέξεις κλειδιά – Keywords
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Μονοξείδιο του αζώτου (ΝΟ), ενδοθηλιακή συνθάση του μονοξειδίου του αζώτου (e-NOS), πολυμορφισμός, έμφραγμα του μυοκαρδίου
Nitric oxide, e-NOS, polymorphism, myocardial infarction
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Λοιποί Όροι – Other Terms
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Άρθρο Article |
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Περίληψη – Summary
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– Nitric oxide (NO), produced by endothelial nitric oxide synthase (e-NOS), plays a key role in the regulation of vascular tone and exerts vasoprotective effects. The Glu298Asp polymorphic variant of e-NOS has been associated with myocardial infarction (MI), but data relating to this variant are lacking, especially in Greece. Accordingly, we examined a possible association between the Glu298Asp polymorphism of the e-NOS gene and MI in a subgroup of the Greek population. The study population consisted of 204 patients with a history of MI and 197 control subjects who are selected from the general population of the greater Athens area. Genotyping was performed with melting curve analysis (Lightcycler) of polymerase chain reaction products from acceptor (5΄end-labeled with LCRed 640) and donor probes (3΄end-labeled with fluorescein) specific for the polymorphism. The risk for MI in Glu298Asp TT was 2.159 (95% confidence interval [CI], 1.079 to 4.319), p<0.05 versus GG+GT and 2.352 (95% CI, 1.140 to 4.855), p<0.05 versus GG. The risk for the T allele was 1.556 (95%CI, 1.090 to 2.221), p<0.05 versus G allele. This risk was higher when the genetic factor was combined with other risk factors for MI, such as male gender, age < 60 years and cigarette smoking. This study indicates that Glu298Asp polymorphism of the e-NOS gene probably increases the risk for MI in the Greek population, especially when combined with other cardiovascular risk factors. |
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Αναφορές – References
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1. Moncada S., Higgs A.: The L-arginine-nitric oxide pathway. N. Engl. J. Med. 329: 2002-2012 (1993)
2. Janssens S.P., Shimouchi A., Quertermous T., Bloch D.B., Bloch K.D.: Cloning and expression of a cDNA encoding human endothelium derived relaxing factor/nitric oxide synthase. J. Biol. Chem. 267: 14519-14522 (1992) 3. Marsden P.A., Schappert K.T., Chen H.S., Flowers M., Sundell C.L., Wilcox J.N., Lamas S., Michel T.: Molecular cloning and characterization of human endothelial nitric oxide synthase. FEBS Lett. 307: 287-293 (1992) 4. Marsden P.A., Heng H.H., Scherer S.W., Stewart R.J., Hall A.V., Shi X.M., Tsui L.C., Schappert K.T.: Structure and chromosomal localization of the human constitutive endothelial nitric oxide synthase gene. J. Biol. Chem. 268: 17478-17488 (1993) 5. Hingorani A.D., Jia H., Stevens P.A., Monteith M.S., Brown M.J.: A common variant in exon 7 of the endothelial constitutive nitric oxide synthase gene. Clin. Sci. 88: 21 (1995) 6. Alpert J.S., Thygesen K., Antman E., Bassand J.P.: Myocardial infarction redefined-a consensus document of the joint European Society of Cardiology/American College of Cardiology Committee for the redefinition of myocardial infarction. J. Am. Coll. Cardiol. 36: 959-969 (2000) 7. Micromix 660 DNA extraction. Genomic DNA extraction from up to 660 microlitres of blood. Talent 8. LightMix® Kit human e-NOS E298D, Cat.-No. 40-0380-16, TIB MOLBIOL 2008 9. Antoniades C., Tousoulis D., Vasiliadou C., Pitsavos C., Chrysohoou C., Panagiotakos D., Tentolouris C., Marinou K., Koumallos N., Stefanidis C.: Genetic polymorphism on endothelial nitric oxide synthase affects endothelial activation and inflammatory response during the acute phase of myocardial infarction. J. Am. Coll. Cardiol. 46: 1101-1109 (2005) 10. Antoniades C., Tousoulis D., Vasiliadou C., Pitsavos C., Toutouza M., Tentolouris C., Marinou K., Stefanidis C.: Genetic polymorphisms G894T on the eNOS gene is associated with endothelial function and vWF levels in premature myocardial infarction survivors. Int. J. Cardiol. 107: 95-100 (2006) |
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Online ISSN 1011-6575
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Articles published in this Journal are Indexed or Abstracted in:
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