Τόμος 13 (1995) – Τεύχος 1 – Άρθρο 1 – Επιθεώρηση Κλινικής Φαρμακολογίας και Φαρμακοκινητικής-Ελληνική Έκδοση – Volume 13 (1995) – Issue 1 – Article 1 – Epitheorese Klinikes Farmakologias και Farmakokinetikes-Greek Edition

Από | | Άρθρο - Article, Αστέριος Σ. Τσιφτσόγλου - Asterios S. Tsiftsoglou, Ιωάννης Βιζιριανάκης, Ιωάννης Βιζιριανάκης, Πλήρες Κείμενο στα Ελληνικά - Full Text in Greek

 

Τίτλος – Title

Ο πιθανός ρόλος της σταθερότητας του RNA του Messenger (mRNA) στο γονίδιο. Έκφραση και ανάπτυξη νέων Θεραπευτικών

Potential Role of Messenger RNA (mRNA) Stability in Gene. Ex­pression and Development of new Therapeutics
Συγγραφέας – Author

Ιωάννης Βιζιριανάκης και Αστέριος Σ. Τσιφτσόγλου

Eργαστήριο Φαρμακολογίας, Τμήμα Φαρμακολογίας-Φαρμακογνωσίας, Τμήμα Φαρμακευτικής, Αριστοτέλειο Πανεπιστήμιο Θεσσαλονίκης, 540 06 Θεσσαλονίκη

Ioannis Vizirianakis and Asterios S. Tsiftsoglou

Laboratory of Pharmacology, Division of Pharma­cognosy-Pharmacology, Department of Pharma­ceutical Sciences, Aristotle University of Thessa­loniki, 540 06 Thessaloniki, Greece
Παραπομπή – Citation

Βιζιριανάκης,Ι., Τσιφτσόγλου,Α.Σ. : O Püëoò της Σταθερότητας των Moñßùí τoυ Aããåëéáöüñïõ RNA (mRNA) στον Έλεγxo της Γενετικής Έκφρασης στα Eõêáñõùôéêá Kýôôáñá και στην AíÜðôõîç Θεραπευτικών Oõóéþí , Επιθεώρηση Κλιν. Φαρμακολ. Φαρμακοκινητ. 14 : 7-32 (1996)

Vizirianakis,I., Tsiftsoglou,A.S. : Potential Role of Messenger RNA (mRNA) Stability in Gene. Ex­pression and Development of new Therapeutics, Epitheorese Klin. Farmakol. Farmakokinet. 14 : 7-32 (1996)
Ημερομηνία Δημοσιευσης – Publication Date
15-02-1995
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Ελληνικά – Greek
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Λέξεις κλειδιά – Keywords

Σταθερότητα mRNA, έκφραση γονιδίων, ευκαρυωτικά κύτταρα

mRNA stability, gene expression, eukaryotic cells
Λοιποί Όροι – Other Terms

Άρθρο

Article
Περίληψη – Summary

Eðåé­äÞ σε πολ­λά α­να­πτυ­ξια­κά συ­στή­μα­τα ζω­ϊ­κών κυτ­τά­ρων η δια­θέ­σι­μη προς με­τά­φρα­ση πο­σό­τη­τα του mRNA πολ­λών πρω­τε­ϊ­νών με­τα­βάλ­λε­ται, υ­πάρ­χει έ­ντο­νο εν­δια­φέ­ρον για τον τρό­πο με τον ο­ποί­ο ρυθ­μί­ζε­ται αυ­τή η πο­σό­τη­τα του mRNA (steady-state level). Ση­μα­ντι­κό ρό­λο στη δια­δι­κα­σί­α αυ­τή φαί­νε­ται να δια­δρα­μα­τί­ζουν τό­σο ο ρυθ­μός πα­ρα­γω­γής του mRNA κα­τά τη με­τα­γρα­φή του DNA, ό­σο και η τα­χύ­τη­τα ω­ρί­μαν­σης (RNA splicing) και η στα­θε­ρό­τη­τα (stability) του ι­δί­ου του mRNA, δη­λα­δή ο ρυθ­μός διά­σπα­σής του. Tá τε­λευ­ταί­α χρό­νια, σε ο­ρι­σμέ­νες του­λά­χι­στον πε­ρι­πτώ­σεις, διε­πι­στώ­θη ό­τι τα mRNAs δια­θέ­τουν πρω­το­τα­γείς και δευ­τε­ρο­τα­γείς δο­μές και δια­μορ­φώ­σεις, που δια­δρα­μα­τί­ζουν ση­μα­ντι­κό ρό­λο στη διά­σπα­ση τους, για­τί τους ε­πι­τρέ­πουν να α­ντι­δρούν ε­κλε­κτι­κά και να συν­δέ­ο­νται με πρω­τε­ΐ­νες. Tï άρ­θρο αυ­τό έ­χει ως σκοπό να πα­ρου­σιά­σει τις βα­σι­κές και σύγ­χρο­νες α­πό­ψεις, κα­θώς και τους μη­χα­νι­σμούς που κα­θο­ρί­ζουν τη στα­θε­ρό­τη­τα μο­ρί­ων mRNA κα­τά την κυτ­τα­ρι­κή α­να­πα­ρα­γω­γή και δια­φο­ρο­ποί­η­ση. Eðé­ðëÝ­ïí, το άρ­θρο αυ­τό πε­ρι­γρά­φει αφ, ε­νός το ρό­λο της στα­θε­ρό­τη­τας ο­ρι­σμέ­νων μο­ρί­ων mRNA σε συ­γκε­κρι­μέ­νες πα­θο­φυ­σιο­λο­γι­κές δια­τα­ρα­χές και τη με­θο­δο­λο­γί­α με την ο­ποί­α προσ­διο­ρί­ζε­ται η στα­θε­ρό­τη­τα τους σε αυ­τές τις κα­τα­στά­σεις και αφ, ε­τέ­ρου τη ρύθ­μι­ση των ε­πι­πέ­δων μο­ρί­ων mRNA α­πό φάρ­μα­κα. TÝ­ëïò, πε­ρι­γρά­φο­νται πει­ρα­μα­τι­κά δε­δο­μέ­να του ερ­γα­στη­ρί­ου μας που α­φο­ρούν συ­σχε­τι­σμούς με­τα­ξύ της με­θυ­λί­ω­σης και της στα­θε­ρό­τη­τας μο­ρί­ων mRNA κα­τά τη δια­φο­ρο­ποί­η­ση νε­ο­πλα­σμα­τι­κών κυτ­τά­ρων με φαρ­μα­κευ­τι­κές ου­σί­ες (ε­πα­γω­γείς).

Most recently, the process of mRNA decay in eukaryotic cells has been widely recog­nized as a major regulatory step in gene expres­sion. This paper is a comprehensive review focus­ing on the potential role of mRNA stability during growth and differentiation of eukaryotic cells, two major processes that are characterized by dramatic changes in the steady-state accumu­lation of mRNA. Furthermore, it provides evidence indicating the different cis- and trans-acting fac­tors which seem to implicate and regulate the process of mRNA decay in eukaryotic cells. Dif­ferent structural elements on mRNA molecules, (5′ cap structure, poly(A)-tail, sequences on 5′ and 3′ UTRs, e.t.c.), in closely co-ordinated inter­action with different isolated and partly or wholly characterized trans-acting factors, (CBPs, PABPs, AU-binding factors, IRE-BPs, e.t.c.), lead into specific and developmentally regulated mRNA de­cay by specific and partly unknown nucleases. The molecular mechanism(s) of degradation of specific mRNAs are presented and especially those responsible for regulation of mRNA stability of tubulins and non-polyadenylated histones as well as mRNAs coded for transferrin receptor via iron availability. Finally, some future perspectives in this rapidly expanding field are presented and discussed in close relation with the development of agents that affect RNA stability and may be used as therapeutics.

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