|
Τίτλος – Title
|
Φαρμακολογικό Preconditioning στην Απόκριση των Ευκαρυωτικών Κυττάρων στο Οξειδωτικό Stress: Βήμα προς Βήμα Αξιολόγηση της Μεθοδολογίας Pharmacological Preconditioning in the Oxidative Stress Response of Eukaryotic Cells: In Process Method Validation |
|
|
Συγγραφέας – Author
|
Ευγενία X. Γιαννακοπούλου1* και Αικατερίνη Τυλιγάδα1 1Εργαστήριο Πειραματικής Φαρμακολογίας, Ιατρική Σχολή, Πανεπιστήμιο Αθηνών, Μ. Ασίας 75, 11527 Αθήνα, Ελλάς, *Μεταδιδακτορική υπότροφος του Ιδρύματος Κρατικών Υποτροφιών Eugenia Ch. Yiannakopoulou1* and Ekaterini Tiligada1 1Department of Pharmacology, Medical School University of Athens, M. Asias 75, GR-11527 Athens, Greece; *Postdoctoral Research Scholar of the National Scholarship Foundation |
|
|
Παραπομπή – Citation
|
Γιαννακοπούλου,Ε.-Χ., Τυλιγάδα,Α, : Φαρμακολογικό Preconditioning στην Απόκριση των Ευκαρυωτικών Κυττάρων στο Οξειδωτικό Stress: Βήμα προς Βήμα Αξιολόγηση της Μεθοδολογίας, Επιθεώρηση Κλιν. Φαρμακολ. Φαρμακοκινητ. 25: 30-32 (2007)
Yiannakopoulou,E.-Ch., Tiligada,E. : Pharmacological Preconditioning in the Oxidative Stress Response of Eukaryotic Cells: In Process Method Validation, Epitheorese Klin. Farmakol. Farmakokinet. 25: 30-32 (2007)
|
|
|
Ημερομηνία Δημοσιευσης – Publication Date
|
2007 – 2007
|
|
|
Γλώσσα Πλήρους Κειμένου –
Full Text Language |
Ελληνικά – Greek |
|
|
Παραγγελία – Αγορά –
Order – Buy |
Ηλεκτρονική Μορφή: pdf (15 €) –
Digital Type: pdf (15 €) pharmakonpress[at]pharmakonpress[.]gr |
|
|
Λέξεις κλειδιά – Keywords
|
–
Oxidative stress, pharmacological preconditioning, N-acetyl-cysteine, glutathione, stress response, yeast
|
|
|
Λοιποί Όροι – Other Terms
|
Άρθρο Article |
|
|
Περίληψη – Summary
|
–
Cellular damage caused by free radicals is referred to as oxidative stress (OS). OS is implicated in a number of pathophysiological states, including cardiovascular diseases, diabetes, Parkinson’s and Alzheimer disease, inflammation and cancer, as well as in ageing processes; their progress being frequently modified by the pharmacological modulation of the OS response (pharmacological preconditioning). The aim of this study was the in process validation of methodological issues that arose during the investigation of the effect of pharmacological preconditioning on the OS response. Experiments were carried out using the unicellular eukaryote Saccharomyces cerevisiae ATCC 2366, which is an accepted experimental model for the characterisation of the highly conserved stress response. Various concentrations of the oxidizing agent H2O2 were administered for different time periods. The OS response was evaluated by determining cell viability via the ability of yeast cells to form colony forming units after the OS. However, prior to reaching firm conclusions on the consequences of the response in eukaryotic cells, the optimal experimental conditions needed to be determined “in process” for the elimination of false positive and false negative results. The optimal experimental conditions were established as follows: (a) for the lethal OS, incubation of post-logarithmic yeast cultures in the presence of 1.5mM H2O2 for 1h; (b) for the mild OS, incubation in the presence of 0.15mM H2O2 for 1h. The validity of these conditions was confirmed through the investigation of pharmacological preconditioning with the classical antioxidants N-acetyl-cysteine and glutathione.
|
|
|
Αναφορές – References
|
1. Klatt P., Lamas S.: Regulation of protein function by S-glutathiolation in response to oxidative and nitrosative stress. Eur. J. Biochem. 267: 4928-4944 (2000)
2. Costa V., Moradas Ferreira P.: Oxidative stress and signal transduction in Saccharomyces cerevisiae: insights into ageing, apoptosis and disease. Mol. Aspects Med. 22: 217-246 (2001) 3. Tiligada E., Miligkos V., Delitheos A.: Cross talk between cellular stress, cell cycle and anticancer agents: Mechanistic aspects. Curr. Med. Chem. Anti-cancer Agents 2: 553-566 (2002) 4. Tiligada E., Miligkos V., Ypsilantis E., et al.: Molybate induces thermotolerance in yeast. Lett. Appl. Microbiol. 29: 77-80 (1999) 5. Yiannakopoulou Ε.Ch., Delitheos A., Tiligada E.: Dose-dependent effect of non-steroidal anti-inflammatory agents on the cellular stress response Epitheor. Klin. Farmacol. Farmakokinet. 23: 39-41(2005) 6. Tiligada E., Giannakakou P., Karavokyros I., et al.: Effect of calcium channel blockers on the actions of various antitumor agents in the yeast Saccharomyces cerevisiae. J. Appl. Bacteriol. 81: 481-485 (1996) 7. Yiannakopoulou Ε.Ch., Tiligada E.: Acetaminophen modulates the oxidative stress response in eucaryotic cells Rev. Clin. Pharmacol. Pharmacokinet. Intern. Ed. 20: 125-127 (2006) 8. Thakare S.R.: Catalytic degradation of methylene blue by Fenton like system: model to the environmental reaction. J. Environ. Sci. 16: 285-7 (2004) 9. Jamieson DJ. Oxidative stress responses of the yeast Saccharomyces cerevisiae. Yeast 14: 1511-1527 (1998) |
|
Online ISSN 1011-6575
• Elsevier’s Bibliographic Databases: Scopus, EMBASE, EMBiology, Elsevier BIOBASE
SCImago Journal and Country Rank Factor
Articles published in this Journal are Indexed or Abstracted in:
• Chemical Abstracts
• Elsevier’s Bibliographic Databases: Scopus, EMBASE, EMBiology, Elsevier BIOBASE
SCImago Journal and Country Rank Factor
Τι είναι η Επιθεώρηση Κλινικής Φαρμακολογίας και Φαρμακοκινητικής-Ελληνική Έκδοση-Οδηγίες προς τους Συγγραφείς
What is Epitheorese Klinikes Farmakologias και Farmakokinetikes-Greek Edition-Instrunctions to Authors
Άρθρα Δημοσιευμένα στην Επιθεώρηση Κλινικής Φαρμακολογίας και Φαρμακοκινητικής-Ελληνική Έκδοση
Articles Published in Epitheorese Klinikes Farmakologias και Farmakokinetikes-Greek Edition
Συντακτικη Επιτροπή-Editorial Board
|
ΕΤΗΣΙΑ ΣΥΝΔΡΟΜΗ – ANNUAL SUBSCRIPTION
|
||
|
Γλώσσα Πλήρους Κειμένου –
Full Text Language |
Ελληνικά – Greek
|
|
|
Παραγγελία – Αγορά –
Order – Buy |
pharmakonpress[at]pharmakonpress[.]gr
|
|
|
pharmakonpress[at]pharmakonpress[.]gr
|
||