Τόμος 25 (2007) – Τεύχος 1 – Άρθρο 16 – Επιθεώρηση Κλινικής Φαρμακολογίας και Φαρμακοκινητικής-Ελληνική Έκδοση – Volume 25 (2007) – Issue 1– Article 16 – Epitheorese Klinikes Farmakologias και Farmakokinetikes-Greek Edition

Αναγέννηση του Μυός μετά από Επίδραση Τοπικού Αναισθητικού. Μορφομετρική Ανάλυση

Μuscle Regeneration following Local Anesthetic Myotoxic Insult. Mor­phometric Evaluation

Ελληνικά – Greek

Άρθρο

Article

Local anesthetic myotoxicity is a compli­cation following muscle infiltration. Although it is con­sidered reversible over a period of one month, cases of permanent damage have been reported. The aim of the present study was the morphometric evaluation of muscle regeneration after local anesthetic infiltration. The left tibialis anterior muscle of ten male Wistar rats was injected with either 100 μl of ropivacaine 0.75% (R075 group) or 100 μl of ropivacaine 0.375% (R0375 group), while the controlateral muscle was used as control. Four weeks later, the rats were sacrificed; the muscles were dissected, frozen, cut into 10 μm thick sections, stained using acid ATPase and examined using light microscope with a computer imaging sys­tem for morphometric analysis. Sections were evalu­ated morphometrically regarding fiber cross-section area and fiber perimeter for the three muscle fiber types (Type I, Type IIa, Type IIb). Differences among groups were evaluated using independent t-test. Con­sidering fiber perimeter, analysis showed greater val­ues for R075 vs control and R0375 vs control for all fiber types, while R075 vs R0375 comparisons did not reveal significant differences for any of the examined fiber types. In conclusion, four weeks after local anes­thetic application regenerated muscle fibers still showed morphological differences regarding mor­phometric parameters. These differences could be either an intermediate step of the regeneration process or a more permanent lesion. Furthermore, they could be associated with impaired function.