Τόμος 30 (2012) – Τεύχος 3 – Άρθρο 2 – Επιθεώρηση Κλινικής Φαρμακολογίας και Φαρμακοκινητικής-Ελληνική Έκδοση – Volume 30 (2012) – Issue 3 – Article 2 – Epitheorese Klinikes Farmakologias και Farmakokinetikes-Greek Edition


Τίτλος – Title

Ανδρολογικοί Δείκτες DNA και Εργαστηριακές Τεχνικές Μελέτης
Andrological DNA Indices and Techniques of Study

Συγγραφέας – Author

Μαρία Σ. Βενετίκου
MD, DipEndo, PhD Ενδοκρινολόγος, Καθηγήτρια Παθοφυσιολογίας-Νοσολογίας, Σχολή Επαγγελμάτων Υγείας και Πρόνοιας (ΣΕΥΠ), Τμήμα Βασικών Ιατρικών Μαθημάτων, Ανώτατο Τεχνολογικό Εκπαιδευτικό Ίδρυμα (ΑΤΕΙ), Αιγάλεω, Αθήνα, Ελλάς –
M. S. Venetikou
MD, DipEndo, PhD, Professor of Pathophysiology and Nosology (Medicine), School of Health and Caring Professions, Department of Basic Sci- (Lessons), Highest Technological Education of Athens (ATEI), Aigaleo, Athens, Hellas

Παραπομπή – Citation

Βενετίκου Μ. Σ.: Ανδρολογικοί Δείκτες DNA και Εργαστηριακές Τεχνικές Μελέτης, Επιθεώρηση Κλιν. Φαρμακολ. Φαρμακοκινητ. 30: 169-172 (2012)
Venetikou M. S.: Andrological DNA Indices and Techniques of Study, Epitheorese Klin. Farmakol. Farmakokinet. 30: 169-172 (2012)

Ημερομηνία Δημοσιευσης – Publication Date
2 Δεκεμβρίου 2012 – 2012-12-02
Γλώσσα Πλήρους Κειμένου –
Full Text Language

Ελληνικά – Greek

Παραγγελία – Αγορά –
Order – Buy
Λέξεις κλειδιά – Keywords
Χρωματίνη, σπέρμα, οξειδωτικό στρες , ανώμαλο packaging της χρωματίνης, απόπτωση
DNA, chromatin, sperm, oxidative stress, packaging, apoptosis
Λοιποί Όροι – Other Terms

άρθρο επισκόπησης, υπογόνιμοι άνδρες, κατεστραμμένο DNA, σπερματική λειτουργία, δείκτης τμηματοποίησης  (DFI) του σπέρματος, υποβοηθούμενη αναπαραγωγή, ελαττωματική παραγωγή αντιδραστικών οξυγόνων (ROS), έλλειψη πρωταμίνης, μέτρηση TUNEL, μονοκυτταρική ηλεκτροφόρηση (μέτρηση Comet), τεχνική του πορτοκαλί της ακριδίνης (AOT), μέτρηση της δομής της χρωματίνης του σπέρματος (SCSA), ηλεκτροφόρηση PAGE
review article, fragmentation index) (DFI), Reactive Oxygen Species (ROS), Acridine Orange Technique (AOT), Sperm Chromatin Structure Assay (SCSA), Polyacrylamide Gel Electrophoresis (PAGE)

Περίληψη – Summary

Το άρθρο πραγματεύεται τις διαταραχές του ανδρικού DNA (σπέρματος) και τις τεχνικές προσέγγισης στην μελέτη αυτών.

This article reviews the biological disturbances of the male DNA (sperm) and the techniques used in their study.

Αναφορές – References
1. Ahmadi A., Ng S.-C.: Developmental capacity of damaged spermatozoa. Hum. Reprod. 14: 2279-2285 (1999)
2. Saleh R.A., Agarwal A., Nelson D.R., Nada E.A., El-Tonsy M.H., Alvarez J.G., Sharma R.K.: Increased sperm nuclear DNA damage in normozoospermic infertile men: a prospective study.  Fertil. Steril. 78: 313-318 (2002)
3. Aitken R.J., De Iuliis G.N.: Origins and consequences of DNA damage in male germ cells. Reprod. Biomed. Online 14: 727-733 (2007)
4. Ahmadi A., Ng S.-C.: Fertilizing ability of DNA-damaged spermatozoa.  J. Exp. Zool. 284: 696-704 (1999)
5. Cho C., Jung-Ha H., Willis W.D., Goulding E.H., Stein P., Xu Z., Schultz R.M., Hecht N.B., Eddy E.M.: Protamine 2 deficiency leads to sperm DNA damage and embryo death in mice.  Biol. Reprod. 69: 211-217 (2003)
6. Evenson D.P., Jost L.K., Marshall D., Zinaman M.J., Clegg E., Purvis K., de Angelis P., Claussen O.P.: Utility of the sperm chromatin structure assay as a diagnostic and prognostic tool in the human fertility clinic. Hum. Reprod. 14: 1039-1049 (1999)
7. Agarwai A., Said T.M.: Role of sperm chromatin abnormalities and DNA damage in male infertility. Hum. Reprod. Update 9: 331-345 (2003)
8. Gandini L., Lombardo F., Paoli D., Caruso F., Eleuteri P., Leter G., Ciriminna R., Culasso F., Lenzi A., Spano M.: Full-term pregnancies achieved with ICSI despite high levels of sperm chromatin damage. Hum. Reprod. 19: 1409-1417 (2004)
9. Sergerie M., Laforest G., Bujan L., Bissonette F., Bleau G.: Sperm DNA fragmentation: threshold value in male fertility.  Hum. Reprod. 20: 3446-3451 (2005)
10. Sergerie M.: The clinical utility of sperm DNA integrity testing. Fertil. Steril. 86: S35-37 (2006)
11. Erenpreiss J., Spano M., Erenpreisa J., Bungum M., Giwercman A.: Sperm chromatin structure and male fertility: biological and clinical aspects. Asian J. Androl. 8: 11-29 (2006)
12. Zini A., Libman J.: Sperm DNA damage: importance in the era of assisted reproduction. Curr. Opin. Urol. 16: 428-434 (2006)
13. Evenson D.P., Wixon R.: Clinical aspects of sperm DNA fragmentation detection and male infertility. Theriogenology 65: 979-991(2006)
14. Corczyca W., Traganos F., Jesionowska H., Darzyn-kiewicz Z.: Presence of DNA strand breaks and increased sensitivity of DNA in situ to denaturation in abnormal human sperm cells: analogy to apoptosis of somatic cells.  Exp. Cell. Res. 207: 202-205 (1993)
15. Manicardi G.C., Bianchi P.G., Pantano S., Azzoni P., Bizzaro D., Bianchi U., Sakkas D.: Presence of endogenous nicks in DNA ejaculated human spermatozoa and its relationship to chromomycin A3 accessibility. Biol. Reprod. 52: 864-867 (1995)
16. Sakkas D., Mariethoz E., Manicardi G., Azzoni P., Bizzaro D., Bianchi P.G., Bianchi U.: Origin of DNA damage in ejaculated human spermatozoa. Rev. Reprod. 4: 31-37 (1999)
17. Sakkas D., Moffatt O., Manicardi G., Mariethoz E., Tarozzi N., Bizzaro D.: Nature of DNA damage in ejaculated human spermatozoa and the possible involvement of apoptosis. Biol. Reprod. 66: 1061-1067 (2002)
18. Lopes S., Jurisicova A., Sun J.G., Casper R.F.: Reactive oxygen species potential cause for DNA fragmentation in human spermatozoa.  Hum. Reprod. 13: 896-900 (1998)
19. Kodama H., Yamaguchi R., Fukuda J., Kasai H., Tanaka T.: Increased oxidative deoxyribonucleic acid damage in the spermatozoa of infertile male patients. Fertil. Steril. 68: 519-524 (1997)
20. Moustafa M.H., Sharma R.K., Thornton J., Mascha E., Abdel-Hafez M.A., Thomas A.J.,Jr, Agarwal A.: Relationship between ROS production, apoptosis and DNA denaturation in spermatozoa from patients examined for infertility. Hum. Reprod. 19: 129-138 (2004)
21. Oliva R., Dixon G.H.: Vertebrate protamine genes and the histone-to-protamine replacement reaction. Prog. Nucleic Acid Res. Mol. Biol. 40: 25-94 (1991)
22. Irvine D.S., Twigg J.P., Gordon E.L., Fulton N., Milne P.A., Aitken R.J.: DNA integrity in human spermatozoa: relationships with semen quality. J. Androl. 21: 33-44, (2000)
23. Barroso G., Morshedi M., Oehninger S.: Analysis of DNA fragmentation, plasma membrane translocation of phosphatidylserine and oxidative stress in human spermatozoa. Hum. Reprod. 15: 1338-1344 (2000)
24. Kerr J.F., Wyllie A.H., Currie A.R.: Apoptosis: a basic biological phenomenon with wide-ranging implications in tissue kinetics. Br. J. Cancer 26: 239-257 (1992)
25. Huckins C.: The morphology and kinetics of spermatogonial degeneration in normal adult rats: an analysis using a simplified classification of the germinal epithelium. Anat. Rec, 190: 905-926 (1978)
26. Regaud C.P.: Dégénérescence des cellules séminales chez les mammifères en absence de tout état pathologique. C. R. S. Soc. Biol. Fil. 52: 268-270 (1900)
27. Storh P.: I Efubuch der Histologie und microscopishen Anatomie des Menschen. Gustav Fisher, Jena, 1886
28. Gandini L., Lombardo F., Paoli D., Caponecchia L., Fa-miliari G., Verlengia C., Dondero F., Lenzi A.: Study of apoptotic DNA fragmentation in human spermatozoa. Hum. Reprod. 15: 830-839 (2000)
29. Angelopoulou R., Dadoune J.P.: Apoptose dans la spermatogénèse normale et pathologique. Contracept. Fertil. Sex. 27: 99-106 (1999)
30. Jurisicova A., Lopes S., Meriano J., Oppedisano L., Casper R.F., Varmuza S.: DNA damage in round spermatids of mice with a targeted disruption of the Pp1cgamma gene and in testicular biopsies of patients with non-obstructive azoospermia. Mol. Hum. Reprod. 5: 323-330 (1999)
31. Aitken R.J., Buckingham D., West K., Wu F.C., Zikopoulos K., Richardson D.W.: Differential contribution of leucocytes and spermatozoa to the generation of reactive oxygen species in the ejaculates of oligozoospermic patients and fertile donors. J. Reprod Fertil. 94: 451-462, (1992)
32. Erenpreiss J., Hlevicka S., Zalkalns J., Erenpreisa J.: Effect of leukocytospermia on sperm DNA integrity: a negative effect in abnormal semen samples. J. Androl. 23: 717-723 (2002)
33. Kuhnert B., Nieschlag E.: Reproductive functions of the ageing male. Hum. Reprod. Update 10: 327-339 (2004)
34. Singh N.P., Muller C.H., Berger R.E.: Effects of age on DNA double-strand breaks and apoptosis in human sperm. Fertil. Steril. 80: 1420-1430 (2003)
35. Angelopoulou R., Kyriazoglou M.: Sperm oxidative damage and the role of reactive oxygen species in male infertility. Arch. Hellenic Med. 22: 433-446 (2005)
36. De Iuliis G.N., Wingate J.K., Koppers A.J., McLaughlin E.A., Aitken R.J.: Definitive evidence for the nonmitochondrial production of superoxide anion by human spermatozoa. J. Clin. Endocrinol. Metab. 91: 1968-1975 (2006)
37. Morris ID.: Sperm DNA damage and cancer treatment. Int. J. Androl. 25: 255-261 (2002)
38. Spanò M., Kolstad A.H., Larsen S.B., Cordelli E., Leter G., Giwercman A., Bonde J.P.: The applicability of the flow cytometric sperm chromatin structure assay in epidemiological studies. Asclepios. Hum. Reprod. 13: 2495-2505 (1998)
39. Wyrobek A.J., Eskenazi B., Young S., Arnheim N., Ti-emann-Boege I., Jabs E.W., Glaser R.L., Pearson F.S., Evenson D.: Advancing age has differential effects on DNA damage, chromatin integrity, gene mutations, and aneup-loidies in sperm. Proc. Natl. Acad. Sci. USA 103: 9601-9606 (2006)
40. Aitken R.J., Krausz C.: Oxidative stress, DNA damage and the Y chromosome. Reproduction 122: 497-506 (2001)
41. Virro M.R., Larson-Cook K.L., Evenson D.P.: Sperm chromatin structure assay (SCSA) parameters are related to fertilization, blastocyst development, and ongoing pregnancy in in vitro fertilization and intracytoplasmic sperm injection cycles. Fertil. Steril. 81: 1289-1295 (2004)
42. Henkel R., Hajimohammad M., Stalf T., Hoogendijk C., Mehnert C., Menkveld R., Gips H., Schill W.B., Kruger T.F.: Influence of deoxyribonucleic acid damage on fertilization and pregnancy. Fertil. Steril. 81: 965-972 (2004)
43. Morris I.D., Ilott S., Dixon L., Brison D.R.: The spectrum of DNA damage in human sperm assessed by single cell gel electrophoresis (Comet assay) and its relationship to fertilization and embryo development. Hum. Reprod 17: 990-998 (2002)
44. Li Z., Wang L., Cai J., Huang H.: Correlation of sperm DNA damage with IVF and ICSI outcomes: a systematic review and meta-analysis. J. Assist. Reprod. Genet. 23: 367-376 (2006)
45. Benchaib M., Braun V., Lornage J., Hadj S., Salle B., Lejeune H., Guerin J.F.: Sperm DNA fragmentation decreases the pregnancy rate in an assisted reproductive technique. Hum. Reprod. 18: 1023-1028 (2003)
46. Benchaib M., Lornage J., Mazoyer C., Lejeune H., Salle B., Guerin F.J.: Sperm deoxyribonucleic acid fragmentation as a prognostic indicator of assisted reproductive technology outcome. Fertil Steril, 87: 93-100 (2007)
47. Sun J.G., Jurisicova A., Casper R.F.: Detection of deoxyribonucleic acid fragmentation in human sperm: correlation with fertilization in vitro. Biol. Reprod. 56: 602-607 (1997)
48. Sailer B.L., Jost L.K., Evenson D.P.: Mammalian sperm DNA susceptibility to in situ denaturation associated with the presence of DNA strand breaks as measured by the terminal deoxynucleotidyl transferase assay. J. Androl. 16: 80-87 (1995)
49. Anderson D., Dobrzynska M.M., Basaran N.: Effect of various genotoxins and reproductive toxins in human lymphocytes and sperm in the Comet assay. Teratog. Carcinog. Mutagen. 17: 29-43 (1997)
50. Aravindan G.R., Bjordahl J., Jost L.K., Evenson D.P.:  Susceptibility of human sperm to in situ DNA denaturation is strongly correlated with DNA strand breaks identified by single-cell electrophoresis. Exp. Cell. Res. 236: 231-237 (1997)
51. Hughes C.M., Lewis S.E., McKelvey-Martin V.J., Thompson W.: A comparison of baseline and induced DNA damage in human spermatozoa from fertile and infertile men, using a modified comet assay. Mol. Hum. Reprod. 2: 613-619 (1996)
52. Twigg J., Fulton N., Gomez E., Irvine D.S., Aitken R.J.: Analysis of the impact of intracellular reactive oxygen species generation on the structural and functional integrity of human spermatozoa: lipid peroxidation, DNA fragmentation and effectiveness of antioxidants. Hum. Reprod. 13: 1429-1436 (1998)
53. Teyada R.I., Mitchell J.C., Norman A., Marik J.J., Friedman S.: A test for the practical evaluation of male fertility by acridine orange (AO) fluorescence.  Fertil. Steril. 42: 87-91 (1984)
54. Evenson D.P., Larson K.L., Jost L.K.: Sperm chromatin structure assay: its clinical use for detecting sperm DNA fragmentation in male infertility and comparisons with other techniques. J. Androl. 23: 25-43 (2002)
55. Terquem A., Dadoune J.P.: Aniline blue staining of human spermatozoa chromatin. In: Evaluation of Nuclear Maturation in the Sperm Cell (Andre J., ed.)  Evaluation of Nuclear Maturation in the Sperm Cell. Pp. 249-252, Martinus Nijhoff, 1982
56. Hayasaka T., Inoue Y.: Chromomycin A3 studies in aqueous solutions. Spectrophotometric evidence for aggregation and interaction with herring sperm deoxyribonucleic acid. Biochemistry 8: 2342-2347 (1968)

Online ISSN 1011-6575

Άρθρα Δημοσιευμένα σε αυτό το Περιοδικό Καταχωρούνται στα:
Chemical Abstracts

Elsevier’s Bibliographic Databases: Scopus, EMBASE, EMBiology, Elsevier BIOBASE
SCImago Journal and Country Rank Factor

Articles published in this Journal are Indexed or Abstracted in:
• Chemical Abstracts
• Elsevier’s Bibliographic Databases: Scopus, EMBASE, EMBiology, Elsevier BIOBASE
SCImago Journal and Country Rank Factor

Άρθρα Δημοσιευμένα στην Επιθεώρηση Κλινικής Φαρμακολογίας και Φαρμακοκινητικής-Ελληνική Έκδοση
Articles Published in Epitheorese Klinikes Farmakologias και Farmakokinetikes-Greek Edition

Συντακτικη Επιτροπή-Editorial Board

ΕΤΗΣΙΑ ΣΥΝΔΡΟΜΗ – ANNUAL SUBSCRIPTION
Γλώσσα Πλήρους Κειμένου –
Full Text Language
Ελληνικά – Greek
Παραγγελία – Αγορά –
Order – Buy
pharmakonpress[at]pharmakonpress[.]gr
pharmakonpress[at]pharmakonpress[.]gr
Προσθέστε στους σελιδοδείκτες το μόνιμο σύνδεσμο.

Τα σχόλια είναι απενεργοποιημένα.