Open Access Research
Antiamoebic and phytochemical studies of two medicinal plants: traditional recipes used against amoebiasis
Ruaa Aziz Jassim1,*
, Asmaa Mahdi Hussein2, Dania F Alsaffar3
1Department of Pharmacognosy, College of Pharmacy, Al-Bayan University, Baghdad
2Alturath University, Department of Pharmacy (Pharmacognosy), Baghdad-Iraq
3Department of Basic Science, College of Dentistry, Ibn-Sina University of Medical and Pharmaceutical Sciences, Baghdad, Iraq
*Corresponding author
Ruaa Aziz Jassim, Department of Pharmacognosy, college of Pharmacy, Al-Bayan University, Baghdad-Iraq; Tel.:+9647815996067; email; ruaa.aziz@albayan.edu.iq
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Published: 27 December 2024; https://doi.org/10.61873/OEPQ3227
Abstract
Background: Amoebiasis, an intestinal infection caused by the parasitic amoeba Entamoeba histolytica, presents varying symptoms from none to severe. Traditional non-herbal treatments often carry adverse effects, prompting interest in herbal remedies. Aim: This study evaluates the effectiveness of Tamarindus indica (T. indica) and Cynodon dactylon (C. dactylon) extracts against E. histolytica. Using the Soxhlet extraction method, extracts were obtained in different solvents and analyzed for percentage yield, phytoconstituents (PCs), quality control parameters, fluorescence, high-performance thin layer chromatography (HPTLC), and Fourier transform infrared (FTIR) spectroscopy. The antiprotozoal study was conducted using the TY1-S33 inoculum of E. histolytica. Results: The methanolic extracts of T.indica and C.dactylon had higher yields (22.2% and 8.11%, respectively) compared to the chloroform extracts. Methanolic extracts revealed the presence of carbohydrates, flavonoids, tannins, and proteins. Quality control tests showed loss on drying (8.1% for T. indica and 6.38% for C. dactylon), total ash value (9.8% and 9.89%), and methanol soluble extractive (22.2% and 12.60%). Fluorescence analysis and HPTLC confirmed the presence of PCs. FTIR analysis identified O-H & N-H bonds at 3210.04, 2916.78, and 2830 cm-1 for T. indica, and N-H & C=O peaks at 3402.2 & 2215 cm-1 for C. dactylon. Conclusion: The antiprotozoal study demonstrated significant inhibition of trophozoites by T. indica (98.2%) and C. dactylon (97.7%). Thus, both plant extracts were found to be safe and effective for treating amoebiasis, suggesting improved patient compliance with herbal treatments.
Keywords: amoebiasis, Cynodon Dactylon, Tamarindus Indica, E. histolytica
Please cite as:
Jassim R. A., Hussein A. M., Alsaffar D. F.: Antiamoebic and phytochemical studies of two medicinal plants: traditional recipes used against amoebiasis. Epitheorese Klin. Farmakol. Farmakokinet. 42(Sup1): 61-73 (2024). DOI: 10.61873/OEPQ3227
pISSN 1011-6575 • eISSN 2945-1914